mpidb The Microbial Protein Interaction Database mignolet-2010-1 PdhS, an old-pole-localized histidine kinase, recruits the fumarase FumC in Brucella abortus. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Mignolet J., Van der Henst C., Nicolas C., Deghelt M., Dotreppe D., Letesson J.J., De Bolle X. Brusella melitensis ORFeome J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation mignolet-2010-2 PdhS, an old-pole-localized histidine kinase, recruits the fumarase FumC in Brucella abortus. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Mignolet J., Van der Henst C., Nicolas C., Deghelt M., Dotreppe D., Letesson J.J., De Bolle X. Brusella melitensis ORFeome J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation divk_brume Polar-differentiation response regulator DivK divK BMEII0659 protein protein brume Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) MTKSVMIVEDNELNMKLFRDLIEASGYETIRTRSGLEALDLAREHHPDLILMDIQLPEVSGLEVTKWLKDDEELRHIPVIAVTAFAMKGDEERIRQGGCEAYISKPISVPRFIETIKSYLGDA pdhs_brua2 Cell-division control histidine kinase PdhS pdhS BAB1_1621 protein protein brua2 brua2 MSGSYPFIDIAALDSVREGFARGDAQLVLAHDLSTVLWVNGPGAKLFGYNRVEDLIEGQLDLPVATRRQIAAFSSENTSAPSAVAVRLGGGLRSELTHLHVSNIKLPDGVAALLVATQMPDNSAEAAISGLGDDSTHIALVDAVGKVVAASPRFALLDISASTLEDLIVEAGDATDRIVKRRIRTGSHSVPGAIARLTDTPALHLLCIVGDAPAQFQTAAEAVPLPDNAEAVLEEILPEQGDAPAQQAQKTHAEQPRPKTFAFDHDAPPARFIWKVGPDGTFSEISPNLAAVVGPNSADIVGRRFSDVANVFGFDTDGSIAALLLERDTWSGKRLLWPVEGTRLRVPVELAALPVYSRDREFLGFRGFGIVRPAEAEADPEEIGLALAGGIPQNRKPRKEPAETARMVGEDDVLALSEEVANDDQPAAVLPKPPLDITPTPGRRDSDKVISLLNSCAQEKVAADQAKFLKEKERATRPEGGLTKTERNAFREIAERLRKQGLANTRAESETPVSETSSIEPVEPTPPVKTRSEPIQPDETALLANLPVPVIIHSGDAIHYVNQALLDITGYESLDDIRSAGGVDVLFNSESDDGETRQSMLLRHADGSEEPVDAHLNAIAWRGGRALMLSLMPVTAADLPAPAELPAANDEEKQALEAHVEELKTILDTATDGVVLIDPEGRIRSMNHSASALFGYERDEAEGKFFSMLFAIESQRAAMDYLHGLSGNGVLSVLNDGREVIGREAKGGFIPLFMTIGKLPHTRGFCAVLRDITQWKRTEEELTNARKEAERASNQKTEFLARISHEIRTPLNAIIGFSELMADEKFGPIGNDRYRDYLRDINRSGNHVLALVNDLLDISKIEAGALDMQFEAVSLNDAIGEAIALMQPQANRERVIIRSSFQSNLPDIVADSRSIKQVALNLLSNAVRFTAPGGQVIVSTSYELNGDVVMRVRDTGIGMSKSEVEQALKPFRQINALERRKAESAKDWRNEGTGLGLPLTKAMVEANRAQFAIDSNPGQGTVVEIVFPPTRVLAD fumc_brume Fumarate hydratase class II fumC Aerobic fumarase Iron-independent fumarase BMEII1051 protein protein brume Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) MAATRTETDTFGPIDVPADRYWGAQTQRSLQNFRIGGERMPLPLVHALGVVKRAAAETNIALGKLDPVLGQVIAVAASEVIEGKLDDHFPLVVWQTGSGTQSNMNANEVISNRAIELLGGEMGSKKPIHPNDHVNMSQSSNDSFPTAIHIATAVETVNRLYPALEHLTKALKVKEEAFKDIIKIGRTHTQDATPVTLGQEFSGYRAALEYARHRLEQSLADVFLLAQGGTAVGTGLNAPVGFDKGFAEAVSEITGLSFKTAPNKFEALASHGAVLNFHGSLNALAADLFKIANDIRFLGSGPRSGLGELSLPENEPGSSIMPGKVNPTQAEAMTMVATQVFGNQTAVTVAASQGHFELNVFKPVIAYNVLQSIRLLSDTMVSFADHCVEGIEPNTARIKELLERSLMLVTALAPAIGYDNAARIAKTAHKNGTTLREEALASGLVSEEDYDRLVRAERMIAPQ pdhs-divk-2 1337353 n/a 1337355 unspecified role unspecified role prey prey not-defined-132021330 1337356 unspecified role unspecified role bait bait physical association physical association false false false pdhs-fumc-2 1337353 n/a 1337357 unspecified role unspecified role prey prey not-defined-570990175 1337356 unspecified role unspecified role bait bait physical association physical association false false false divk-pdhs 1337354 n/a 1337355 unspecified role unspecified role bait bait not-defined-132021330 1337356 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1A fumc-pdhs 1337354 n/a 1337357 unspecified role unspecified role bait bait not-defined-570990175 1337356 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1A