mpidb The Microbial Protein Interaction Database lorenzini-2010-1 Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation lorenzini-2010-2 Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation lorenzini-2010-3 Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). ecolx Escherichia coli 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation lorenzini-2010-4 Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog). ecolx Escherichia coli pull down pull down western blot western blot Immuno blot Immuno blot Lorenzini E., Singer A., Singh B., Lam R., Skarina T., Chirgadze N.Y., Savchenko A., Gupta R.S. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation o84677_chltr CT_670 protein protein chltr chltr MVRYPLEPVLSIKKDRVDRAEKVVKEKRRLLELEQEKLRERESERDKVKNHYMQKIRQLREQLDDGTTSDAILKMKAYIKVVAIQLSEEEEKVNKQKENVLAASKELERAEVELTKRRKEEEKTRLHKEEWMKEALKEEARQEEKEQDEMGQLLHQLHKQKQRESGEN y671_chltr Uncharacterized protein CT_671 CT_671 protein protein chltr chltr MELNKTSESLFSAKIDHNHPRTEAHEPRDQREVRVFSLEGRSSTRQEKADRMPGRTSSRQESSKGSEEGAVHESTAGVSSKEEEESKGDGFFTGGNPTSGMALVETPMAVVSEAMVETSTMTVSQVDLQWVEQLVTSTVESLLVADIDGKQLVEIVLDNSNTVPAAFCGANLTLVQTGEEISVSFSNFVDQAQLTEATQLVQQNPKQLVSLVESLKARQLNLTELVVGNVAVSLPTIEKIETPLHMIAATIRHHDQEGDQEGEGRQDQHQGQHQEKKVEEAHI o84677-o84677-2 1337269 not-defined-2009559749 1337273 unspecified role unspecified role neutral component neutral component n-terminal O84677-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-345146817 1337273 unspecified role unspecified role neutral component neutral component n-terminal O84677-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 2A o84677-o84677-3 1337270 not-defined-1850263791 1337273 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false not-defined-180533372 1337273 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Figure 3E and 3F o84677-y671 1337271 not-defined-1382420455 1337273 unspecified role unspecified role bait bait not-defined-1698442421 1337274 unspecified role unspecified role prey prey physical association physical association false false false Table 3 y671-o84677 1337272 not-defined-1752562812 1337274 unspecified role unspecified role bait bait n-terminal strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false not-defined-1155552374 1337273 unspecified role unspecified role prey prey n-terminal O84677-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 5