mpidb The Microbial Protein Interaction Database rippa-2010-3 The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli. ecolx Escherichia coli lambda two hybrid lambda repressor two hybrid BacterioMatch BacterioMatch lambda two hybrid predetermined predetermined participant predetermined Rippa V., Cirulli C., Di Palo B., Doti N., Amoresano A., Duilio A. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation rippa-2010-1 The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli. in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Rippa V., Cirulli C., Di Palo B., Doti N., Amoresano A., Duilio A. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation rippa-2010-2 The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli. ecolx Escherichia coli anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rippa V., Cirulli C., Di Palo B., Doti N., Amoresano A., Duilio A. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation rpoa_ecoli DNA-directed RNA polymerase subunit alpha rpoA pez phs sez b3295 Transcriptase subunit alpha RNA polymerase subunit alpha JW3257 protein protein ecoli Escherichia coli (strain K12) MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIHSEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE rl2_ecoli 50S ribosomal protein L2 rplB Large ribosomal subunit protein uL2 JW3279 b3317 protein protein ecoli Escherichia coli (strain K12) MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK rpoa-rpoa-1 1337049 not-defined-977027451 1337052 unspecified role unspecified role bait bait not-defined-2029964241 1337052 unspecified role unspecified role prey prey physical association physical association false false false Table 3 rpoa-rplb-1 1337049 not-defined-212855445 1337053 unspecified role unspecified role prey prey not-defined-902487462 1337052 unspecified role unspecified role bait bait physical association physical association false false false Table 3 rpoa-rplb-2 1337050 not-defined-212855445 1337053 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) not-defined-902487462 1337052 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli physical association physical association false false false Figs 1, 2 rpoa-rplb 1337051 not-defined-602677711 1337053 unspecified role unspecified role prey prey p60422-- P60422-- myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-924669413 1337052 unspecified role unspecified role bait bait p0a7z4-- P0A7Z4-- t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3