mpidb The Microbial Protein Interaction Database fraiberg-2010-1 Discovery and characterization of cadherin domains in Saccharophagus degradans 2-40. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Fraiberg M., Borovok I., Weiner R.M., Lamed R. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation fraiberg-2010-2 Discovery and characterization of cadherin domains in Saccharophagus degradans 2-40. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Fraiberg M., Borovok I., Weiner R.M., Lamed R. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation q21ml9_sacd2 Sde_0798 protein protein strain 2-40 / atcc 4 Saccharophagus degradans MSSNVDSENKYFSKKYLTVKKVAQIALPVLAAFSGSASRGSNVSSVGRFSSVATKSYALNEVRLDPQSSAKDGAGLSEDSDRRSVFSAFTYRQNSTQRIEKKNEVNDAISYLEKSGLLTPADSVRSNLSGDVYGLVGRKSFAHESQFDHCHGSFGSTYNTNTTTVTEVSCTASWGGVWETASPNPVVSATSIQNKTATSFDVAVTMSKASTVYAVLLESSKPAPTNAQVEAGTDGSGSAAATTKSAAASNSVTLSFTGLDSNKNYLVYLIGKDGSNNVTTTPAQVNPAYEAGLISNAGTWKFPKTRVDSSGNIYLAHASSGQINFLEWNGSGFSAVTTITAADVAGRTNMGWGNEHRVDYELDSSGNIHVVFNASTSQWAVDNDPFYGVYDGVNWTFTNIGETGYQGGRFDLFLDENDKAHLSFTLTPNADQVKYATNASGSWVINDVITATNDGTDEIHDTYCVVDSSGDAHIFYRREDLQNSSQDNYYYTSSSDNFATHTKVIDGKADAKYYLMANVSIDSSDKIHYAYSNETDGDSFYVTNESGSWVSTEITHASHSILTVYDMEEEGGVYYFASASGSTYFFNSYNGTDWVDGFDFSLTGSLNDRVAVSDAADRIMVVSESSAGWEIHYHSATIASYITPPVSNEAPTITGLSATFSTNEDAAAYLDMSAVTLADTEDDTVTLTLAVNSGALAASDGDGTTAGVTIANSGSASMTLTGAVADLNTYLDTTTKIQFTPTANISGAVTFTATPSDVTSSGTAGTSSITVSAVNDDPTATGVPTDVAVTEDVQSNIDLSAITFADVDAASGNVVLSIAVTAGTLVASDSGGVTVGGSGGSTITLTGTVANIDTYLNTASNVQYTSAANANGNDAATLSLTANDGGNTGSGGGGDVSLGTINLDITSVDDTPSDITLNASSINESDTSAGADVGTLSSTDADSGSFTYSLVSVGAADTGTCTSDTNNSSFQINTVTLETANVLAAGNYDICVQTSDGNTSFEKSFSVTVVDNIAPIVTITSLSTADSTPALAGTVDDTAAAIQVTVNGANYVASNNANGTWSLADNTIAALTHGTWDVAVTATDGASNVGSDATSNELVVDLEAPASHSVSIDQANIDATNESAMSFTFAGAEVGASFSYSVSDGSTNVTGSGTIASSGQQVTNINVSGLNETTLTLSAILTDVLGNAATAVTDTVVKQYNSAPVITEGASTSVNMSEDANPTAFALTLNASDSDADTITWSIATNGSNGTATVTGTGMTKAISYSPNANYFGVDSFIVEVSDGVATDQITVNVSIASINDAPVFSSTAIVSVNEDSGYSYSITTTDVEADARTVVATTKPSWLTFTDNGNGTASLSGTPTNDEVGNHSVLLTLSDGNASATQSFIVTVANTNDAPVISGTPATTINEDSAYSFTPTVSDVDVGDSFVYSITNQPSWASFSTSTGLLSGTPGNDDVGTTTGIIIGVNDGTTTVNLASFDLTVVNTNDAPVISGTPPTSVDEDSAYSFTPTASDVDVGDVLTYSVANAPSWASFNTSNGELSGTPDNDDVGTSSNIIISVNDGTVSESLASFNLTVVNTNDAPVITGTPSTSINEDSAYTFTPAVSDVDAGDTLVYSITNQPSWASFSTSTGLLSGTPGNDDVGTTTGIIIGVNDGTTTVNLASFNLTVVNTNDAPVISGTPPTSVDEDSTYNFTPTVSDVDVGDVLTYSITNAPSWASFSTSTGQLIGTPENTDVGMDSNIIISVSDGAETVSLASFDIEVSNVNDDPVAANDTAATVEDAAVIVSVLTNDSDVDDALNPASVLIVSSPANGTTSLNAGSGAITYTPNANFNGSDSFTYTVEDATGATSNTATVTISISAENDAPVATSDTASTPEDTPFTIDVLVNDNDIDNGDSVDGTTLAIVSQPVNATASVVSGEIYFEPNEHFNGSTTFTYTVDDQNGATSNVATVLVNVTGVNDLPVALGDSASLDEDGSVEVDVLANDSDIDGTIDPSTVSVLSDASNGGTSVNTTTGVITYTPTANFNGSDTFTYVVQDNDGGSSAATTVSVTVASINDAPNGVADTAALMEDNPTTINVLGNDSDVDGSIVVTSVQIVTGPADGTVEVLANGSITYSPDTNYYGDDSFTYQVQDNEGAWSSETSVNVTVSSVNDAPLANNDSVSTDEDTAVSIDLIANDSDADGLLDSSSLVIVSAPANGALVDNLDGTVTYTPSADYFGSDSFTYQIDDDSGDSSNTAAVTITINPVNDAPQISGTPAASVNEDSVYSYTPISSDIEADDLSFSIENMPSWASFDTSNGALTGTPNNDAVGIYGAIVISVSDGVSTTSTSAFEIEVINTNDLPVLGGTPTPSVSQDQEFNFIPTAVDVDAGDTLTFSINSLPGWASFDPLTGQLSGSPNNAAVGFYTGIVISVSDGTSTVALPAFDITVDNVNDAPVAIADLYSVAEGGVLNGVNVLANDFDIDADGLTVTLISGPDSANSFSLSTSGAVSYMHDGSESIFDQFSYQISDGALLSAVVTVDIDIDAVNDAPVFVTAPNVLSLSQGQSYLYDIGVVDPDSVTNLQLVSGPSWLTLNGSELSGEVPLDESVGLTSVVLRASDSEFTVDQNFDLSVVERETSVVSLSASWIGLPSVVDELLTLKIALNHQQGPAIADGNLFVVLTGSNFDASMKNCTALASNEFDCPVTLNSSGNLSFNLKLTPHSEGDVVVNLDVSDFAGDLVTSKIIDVSPSRRTVNQGDNAFPLDNATAIASIEMKADGNREIAAGTGLGDTVKLLDYETGSDDATVLGEIENRGATKALGVADIDLDGSEDLLVVNTAGDATAVYYQRGELNFVEDSATVSLPYANQGVLQDLNSDNYPELILGGSGFNLYIYENILGVYSQSPYVFTSQKSIKKFAKLKQKPDQAPMSGTLVLASSDALVLIDFAIAAGKVTKVGVKESGEVQSRNKLQGYLRSELSIPGITSIRVADLDGDGLEELIVSTRHENNSSSKSGVTIIEVSGTGQMTEVRKLGNASAENVEIADFNGDGLPDLLIANENNTYQFYHGDGTVSGFSLKDTVIYNESDLVLPNDVNDDGLSDLLIYVAESDRVDLYISHSDGDLGARADLAMNSQVETVSSTSYDIRQLVSVTNSGNQTAQDVIVTIDIPLHFNVVSLPDACLFGDAQIICDLGDLAANQNETLTFVLNGDADVNDEVLQSRVTSAALEVAPENNKSTNSLNGLFESTSAGVKGGASGSGAINFGLLGLLLILGMQQKMKYWRQLTVVSLLLLMCGQSHGEPLLNSERVSVEMGIGSTSSNWSESSFLAELQAVTQDVEITRVKTTRTSWQLVGGYELNNWLSAEAGYQSWGDVDVELSLTTNDPDGVRDVLAKDYPVSGAGPYLGARIQYDVSEGITIYARAGVLAWQGEYDVRVGESVQSIKRDGVDLAASFGVNKSLTDNVAFGLGLNLVELAGETQTQVGLQVRYKFLGKKD q21ff1_sacd2 Sde_3323 protein protein strain 2-40 / atcc 4 Saccharophagus degradans MSHPKKPSLAKAGFKKALRATLPAIALFAGGAGGAGGAFASIRNASQSPVSSRRAISSVGPLSTAVHPLPSTLGNTITPLPQKPARSAFAVFGAGGSVGKQSANKNVASTAVKFLLEQQLLNQKVDGNATVKIASTSGDNDASRRRFAHTLGTATPFSALVCGEPGYDTFVFSGANSANTFCSVSGPGGLTNGTNLAQCQALHDACNAPGHGAANNAPTVSNAPTDVTVTEDTASNLDLSSLVFADSDGDSLTVTLAVSAGTMAASTAGGVTVGGSGTGTLTLAGTAANINTYLDTTSNIQYTSAANANGDNAATLTITPNDGTENGTAANINIDITAVNDDPSATGVPTDITVTEESASNFELSAISFSDVDSSGNVTATITASSGTFAASSGGSVTIGGSGTGTLTLTGTIANINTYLDTASNIQYTGASNVSGNDAATAGLTVNDGDGSGNVSVGTINIDITDVNDEPTLTATASNPTFTEGGAAASLFSGSSASTIESGQTLTGFTLTVTNVTDGSNERLNADGTTIVLTHGTSGTTASNSLSYSVMVIGTTATVSFSGGTVSTAATQTLIDALSYQNNSNSPSTSNRVVTLTSLTDSGSNAGANDNTASLAIASTVTVTETNDEPTLTATGSNPTFTEGGAAASLFSGASASTVESGQTLTGFALTVTNVSDGSSEILNADGTAITLTNANSGTTATNSLSYSVSVSGSTATVTFSGGTLSTAATQTLIDALSYQNNTADPNTSNRVITLTSLTDSGSNTGANDNVAALAVASTVTVTAVNDEPTLTATGSNPTFTEGGAAASLFSGAAASTIEAGQTLTGFTLTVTNVSDGSNERLNADGTTIVLTHGTSGTTASNSLSYSVVVIGTTATVSFSGGTVSTAATQTLVDALSYQNNSNDPDTTNRVVTLTSLTDSGLNTGANDNTASLALASTVTVTALNDEPTLTATGSNPTFTEGGAAASLFSGSAASTVESGQTLTGFTLTVTNLADGSSEILNADGTAIALTDTNSGTTATNSLSYSVSVSGTTATVTFSGGTVSSAATQTLIDALSYQNNSATPDVANRVITLTSVTDSGSNTGANDNVAALALASTVAVESIDDDGDLTAGSGVSEPVNLGYSADTVGEAVNVFDFTLSDGGASDSFDLNISEVVIHVAGTSSDTERAAIVWRLNGDDAANVTGTYSAVADTITFTGLSLVVSNGDSETYTVNAYYSDNTIVTHGNTIELSLDGDTDLTVDSGTQMGATSAVDNDTGLIIIDDIAPVTTSVNVPANATYAASSNLDFTVNFNDTVFVTTGSGTPRLELTVGATTRYANYLSGSGSSALLFRHVTQIGDEDTDGINVNGSLQLNAGTVTDSAGNTVDVTLNSVGSLAAVLVDGIAPTLAEVTAVTTPANDSTPSVTFSTTEAGTLAISGSCGSASEGAVASGNNTISLTQTDNSTALAAGTYSDCTATVTDAAGNTSSALTLTSFTIDLTAPTVAEVTAVTTPVNDSTPDVTISTTEAGTLAVGGSCGSASEGAITSGNTTITLTQADNSTAFTAGTYSDCTITVTDASGNANTPVTLTSFTVDLTAPTVAEVTAVTTPGNDTTPNVTISTDEAGTLAVGGSCGSASEGAITSGNSTIALTQADNSSPLTAGTYADCTITVTDASGNVNTALTLTSFEIDLTAPTLVEVAAVTTPTNDSTPSVTFSTTEAGTLAIGGSCGSASEGAVTSGNNTISLTQTDNSTVLADATYSNCTATVTDAAGNTSSALALTSFVVDTTAATVAEVTAVVTPANDTTPDVTISTTEAGTLVMGGSCGSASAGSMSSGNTTLTLTQADNTSPLAAGTYSDCTVAVTDSSGNTSNTITLTAFTIDVAAPTLAEVTAVATPGNNAMPTVTFSSTEAGTLSVGGSCGSGDEGAITSGNNTITLTQPDNSSALMSNSYSDCTLTVVDASSNTSNVLTISTFVVDVSAPTLDTNSSLSVSEGEADAVISATNLAATDNLSTAANVTYTVAAAPANGTLRLSGSALANSGTFTQDDIANNRLTYDHDGGETTSDSFTFSLADGLSNVNNNSSANFSFAISVAAQNDAPQTTDDAETTDEDNAVMVDVLANDADSDDAINAASVTIVSAPSNGSTSINTATGVITYTPAANFNGGDSFTYTVQDATGATSAAATVSITINSVNDAPVAASDVISTDINTAVSINVIANDTDVDTADAPDANTLVVVNNASNGSAVVSAGQIVYTPTNGFVGNDSFTYTVDDSNGATSNTATVTVTVIDPNVVPVASNDTATTDEDTAVEVNVLANDSDGDGSLVASSVAVATAPSNGSTSVNVATGAITYTPSVNFNGSDSFTYTVEDNLGAASAAATVTITVASVNDAPVAVDDTVSLLEDASLMINVLGNDSDVDGSLAASSVAVISGPASGFVAVDPANGSVTYTPLDDYAGNDSFTYQVNDNDGAASNIATVTLTIDPVNDAPLANNDSADVVIGVDKIINVLVNDDDIDGTLEPASIVVTQQPVQGTLVNNNDGTLTYSANQTVDVTSGDSFSYTIEDNSGAVSNAANVQIQFVPAAAPAVSGTPDADVEEDGSYSFTPDVTVGDSNFNLTYSVTNAPSWLSVNPVTGALTGEPGANDVGVFNNIKLNVTDGVNTVALPAFNLTVVARVDSDSDTITDYQEGKDGTDPMDGSDYLDVTPPQITAPSNIILDAVALFTNVTTSQLLGLAADADTAEIDAAMAALVSDNVDGQGCCDLYPKGAIVDSYKLAPGENNIVWQATDNKNNVAEVTQKVYIRPLVSLSKDTTNVEGGVAKIGIVLNGKAPFYPLQIPYIIDVSSTATSADHDLVAGIATFNSGATSVQLMVNITADGIAENDEQLIVRLDDRTTNAQDLTNGFEADIYDINAGARTVHTLTIVERNIAPQVALVAKQNGVATLLVSPNGGPVTVSSTVVDANVGDTHTYSWATRRGNLVDTDGDTSNGLFVFSPSGLANGAYKLSLTVTDSGGAVDTETINVVVVPAPPVLSAAVDTDNDGIDDETEGTADTDGDGISDYLDNIDAPNVLPETVNETDSFLIECDPGVRCRLGQFALAGRSGGARLDKQDIAQQEDLIEDTDFALQSDLFDFEVADLPSEGQSVSVVIPQQMAIPANGVYRKFNNGVWSTFVEDANNSLHSAAGELGYCPPPGDASWQAGLIQGSYCVQLTIEDGGPNDADGIVNGSVEDPGGIAVQNSQSVSTRGSGGGSLAPVLVVLLGGLAWANRKKRTASTRNKSKNNTHKKIAASVATAVALSLPSEKTQALDWQNIVQNSFGEVSISRASGSHNKSDFVTGMAADGLVVTPTKYDTASTAFHFMLGYNYNEHLAATVGYLDLGKIAVDFDVPSGNSSALPTALKNNYPHTGSGLTLGARYTAQVMQPLMGYAELGAFVWQGEVVSVEGIADTELEGSIDPYFALGASLPLNKDAQIGIKYQYHTLDGQGVSLTGVMVKINF q21ml9-q21ml9 1336914 not-defined-938360507 1336916 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cbm3a fusion fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-1495558572 1336916 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 1336924 1336920 direct interaction direct interaction false false false Fig. 3B q21ff1-q21ml9-2 1336914 not-defined-1919901546 1336917 unspecified role unspecified role bait bait cbm3a fusion Q21FF1-- fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region Q21FF1-2288-2496 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false q21ff1-- Q21FF1-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-1760104776 1336916 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1336933 1336929 direct interaction direct interaction false false false Fig. 3B q21ml9-q21ff1-2 1336914 not-defined-994046114 1336916 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cbm3a fusion fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-1295153429 1336917 unspecified role unspecified role prey prey q21ff1-- Q21FF1-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region Q21FF1-2288-2496 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1336941 1336936 direct interaction direct interaction false false false Fig. 3A q21ff1-q21ff1 1336914 not-defined-2040596957 1336917 unspecified role unspecified role bait bait region Q21FF1-2288-2496 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false cbm3a fusion Q21FF1-- fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false q21ff1-- Q21FF1-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-1997217988 1336917 unspecified role unspecified role prey prey region Q21FF1-2288-2496 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false q21ff1-- Q21FF1-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 1336948 1336944 direct interaction direct interaction false false false Fig. 3A q21ml9-q21ml9-1 1336915 not-defined-1393915210 1336916 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false cbm3a fusion fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-1889317365 1336916 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 1336956 1336953 direct interaction direct interaction false false false Fig. 4B q21ff1-q21ff1-1 1336915 not-defined-506543679 1336917 unspecified role unspecified role bait bait region Q21FF1-2288-2496 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-2122384556 1336917 unspecified role unspecified role prey prey region Q21FF1-2261-2500 his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region Q21FF1-2288-2496 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false cbm3a fusion fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 1336964 1336960 direct interaction direct interaction false false false Fig. 4A q21ml9-q21ff1-1 1336915 not-defined-1320066968 1336916 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-112978189 1336917 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region Q21FF1-2288-2496 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false cbm3a fusion fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 1336972 1336968 direct interaction direct interaction false false false Fig. 4B q21ff1-q21ml9-1 1336915 not-defined-595732707 1336916 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false cbm3a fusion fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-1636170242 1336917 unspecified role unspecified role bait bait region Q21FF1-2261-2500 his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region Q21FF1-2288-2496 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1336981 1336976 direct interaction direct interaction false false false Fig. 4A