mpidb The Microbial Protein Interaction Database wiethaus-2009-1 Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation wiethaus-2009-2 Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. ecolx Escherichia coli pull down pull down anti tag western anti tag western blot anti tag western Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation wiethaus-2009-3 Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation wiethaus-2009-4 Specific interactions between four molybdenum-binding proteins contribute to Mo-dependent gene regulation in Rhodobacter capsulatus. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wiethaus J., Müller A., Neumann M., Neumann S., Leimkühler S., Narberhaus F., Masepohl B. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation mopa_rhoca Molybdenum-pterin-binding protein MopA mopA protein protein rhoca Rhodobacter capsulatus Rhodopseudomonas capsulata MNEQPLIAALSLQRAGAPRVGGDRIRLLEAIARHGTIAGAAREVGLSYKTAWDAVGTLNNLFEQPLVEAAPGGRTGGNARVTEAGQALIAGFGLLEGALTKALGVLEGGVSAPEKALNTLWSLTMRTSNRNTLRCTVTRVTLGAVNAEVELALTDGHSLTAVITERSATEMGLAPGVEVFALIKASFVMLAAGGDPGRISACNRLTGIVAARTDGPVNTEIILDLGNCKSITAVITHTSADALGLAPGVPATALFKASHVILAMP modc_rhoca Molybdenum import ATP-binding protein ModC modC molD protein protein rhoca Rhodobacter capsulatus Rhodopseudomonas capsulata MISARFSGRQGDFTLDAAFDVPGQGVTALFGPSGCGKTTVLRCMAGLTRLPGGHLVVNGVTWQEGRQITPPHRRAVGYVFQEASLFTHLSVRENLVYGLRRARGPLRISEAEVTQLLGIDPLLRRPTATLSGGERQRVAIGRALLSQPELLLMDEPLSALDRISRDEILPYLERLHASLQMPVILVSHDLSEVERLADTLVLMEAGRVRAAGPIAAMQADPNLPLIHRPDLAAVIEGVVIALDPAYGLSTLQVPGGRIVVPGNLGPIGARRRLRVPATDVSLGRHAPTDTTILNALPAVILGAEAAEGYQITVRLALGASGEGASLLARVSRKSFDLLGFQPGEQVVARLKAMALSAPAQTGG d5arw3_rhocb RCAP_rcc03261 protein protein rhocb Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) MKLSARNILAGKVTAVETGNVTTHVKIDIGGTVVTASITNEAAADLALKVGDEACAIIKASDVIVGKN mopb_rhoca Molybdenum-pterin-binding protein MopB mopB protein protein rhoca Rhodobacter capsulatus Rhodopseudomonas capsulata MAATKQGGGDDGRCARGVVLERTGARMGAERVALLAAIGRTGSISAAAREVGLSYKAAWDGVQAMNNLLAAPVVTAAPGGKAGGGAVLTPAGEKLIAAYGAIEAGVAKLLSSFEKSLNLDPAEVLRGLSLRTSARNAWACKVWSVAADDVAAQVRMRLGEGQDLTAVITARSAAEMRLAPGSEVLALVKSNFVLLAGAGVPERLSVRNRVRGRVIERIDAPLSSEVTLDLGGGKTITATITRDSAEMLDLHPGVETTALIKSSHVILALP modc-mopa 1335988 not-defined-1285962442 1335992 unspecified role unspecified role prey prey not-defined-1612196693 1335993 unspecified role unspecified role bait bait physical association physical association false false false figure 2 d5arw3-d5arw3 1335988 not-defined-182765052 1335994 unspecified role unspecified role prey prey not-defined-136891277 1335994 unspecified role unspecified role bait bait physical association physical association false false false figure 2 d5arw3-mopb 1335988 not-defined-1147382368 1335994 unspecified role unspecified role bait bait not-defined-513896855 1335995 unspecified role unspecified role prey prey physical association physical association false false false figure 2 mopb-d5arw3 1335988 not-defined-648793901 1335995 unspecified role unspecified role bait bait not-defined-1426561924 1335994 unspecified role unspecified role prey prey physical association physical association false false false figure 2 mopb-mopb 1335988 not-defined-1401470494 1335995 unspecified role unspecified role bait bait not-defined-1508057975 1335995 unspecified role unspecified role prey prey physical association physical association false false false figure 2 mopb-mopa 1335988 not-defined-1650540649 1335995 unspecified role unspecified role bait bait not-defined-813335467 1335992 unspecified role unspecified role prey prey physical association physical association false false false figure 2 mopa-mopb 1335988 not-defined-2007569757 1335992 unspecified role unspecified role bait bait not-defined-1320872721 1335995 unspecified role unspecified role prey prey physical association physical association false false false figure 2 mopa-mopa 1335988 not-defined-1301949246 1335992 unspecified role unspecified role prey prey region Q08385-- sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-2125848762 1335992 unspecified role unspecified role bait bait region Q08385-- sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1336021 1336019 physical association physical association false false false figure 2 mopa-mopa-4 1335988 not-defined-2125848762 1335992 unspecified role unspecified role bait bait region Q08385-- sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-1301949246 1335992 unspecified role unspecified role prey prey region Q08385-- sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1336026 1336024 physical association physical association false false false figure 2, 5 d5arw3-d5arw3-6 1335989 not-defined-2031107067 1335994 unspecified role unspecified role prey prey region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-417048540 1335994 unspecified role unspecified role bait bait region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 3 mopb-d5arw3-1 1335989 not-defined-451687872 1335994 unspecified role unspecified role prey prey region D5ARW3-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-878562702 1335995 unspecified role unspecified role bait bait region Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 4 mopb-mopa-1 1335989 not-defined-98282271 1335992 unspecified role unspecified role prey prey region Q08385-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-217112780 1335995 unspecified role unspecified role bait bait region Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 4 mopb-mopb-1 1335989 not-defined-556371794 1335995 unspecified role unspecified role prey prey region Q08386-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-533106798 1335995 unspecified role unspecified role bait bait q08386-- Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 4 mopa-mopb-1 1335989 not-defined-2113767967 1335995 unspecified role unspecified role prey prey region Q08386-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1227264478 1335992 unspecified role unspecified role bait bait region Q08385-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 4 mopa-mopa-1 1335989 not-defined-1361928693 1335992 unspecified role unspecified role bait bait region Q08385-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-889649679 1335992 unspecified role unspecified role prey prey q08385-- Q08385-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 4 d5arw3-d5arw3-4 1335990 not-defined-844406815 1335994 unspecified role unspecified role neutral component neutral component region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1304887553 1335994 unspecified role unspecified role neutral component neutral component region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false figure 6 mopb-mopb-4 1335990 not-defined-1382439664 1335995 unspecified role unspecified role neutral component neutral component region Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-1284044637 1335995 unspecified role unspecified role neutral component neutral component region Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false figure 6 mopa-mopa-5 1335990 not-defined-123617964 1335992 unspecified role unspecified role neutral component neutral component region Q08385-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-1433131365 1335992 unspecified role unspecified role neutral component neutral component region Q08385-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false figure 6 d5arw3-d5arw3-5 1335991 not-defined-1304887553 1335994 unspecified role unspecified role neutral component neutral component region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1304887553 1335994 unspecified role unspecified role neutral component neutral component region D5ARW3-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 2.0 direct interaction direct interaction false false false figure 6 mopa-mopa-6 1335991 not-defined-1433131365 1335992 unspecified role unspecified role neutral component neutral component region Q08385-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false figure 6 mopb-mopb-5 1335991 not-defined-1284044637 1335995 unspecified role unspecified role neutral component neutral component region Q08386-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false figure 6