mpidb The Microbial Protein Interaction Database rust-2009-1 The Helicobacter pylori anti-sigma factor FlgM is predominantly cytoplasmic and cooperates with the flagellar basal body protein FlhA. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Rust M., Borchert S., Niehus E., Kuehne S.A., Gripp E., Bajceta A., McMurry J.L., Suerbaum S., Hughes K.T., Josenhans C. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation rust-2009-2 The Helicobacter pylori anti-sigma factor FlgM is predominantly cytoplasmic and cooperates with the flagellar basal body protein FlhA. ecolx Escherichia coli adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Rust M., Borchert S., Niehus E., Kuehne S.A., Gripp E., Bajceta A., McMurry J.L., Suerbaum S., Hughes K.T., Josenhans C. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation o25747_helpy FlgM domain-containing protein HP_1122 protein protein helpy Helicobacter pylori Campylobacter pylori MNIKLKDFTMINAVSSLAPVQSLGNYKRVEKNEKVENNEAALDRVAEIKKAIENNQYKINLHETSHKMAKDLLGIS flha_helpy Flagellar biosynthesis protein FlhA flbA HP_1041 flhA protein protein helpy Helicobacter pylori Campylobacter pylori MANERSKLAFKKTFPVFKRFLQSKDLALVVFVIAILAIIIVPLPPFVLDFLLTISIALSVLIILIGLYIDKPTDFSAFPTLLLIVTLYRLALNVATTRMILTQGYKGPSAVSDIITAFGEFSVSGNYVIGAIIFSILVLVNLLVVTNGSTRVTEVRARFALDAMPGKQMAIDADLNSGLIDDKEAKKRRAALSQEADFYGAMDGASKFVKGDAIASIIITLINIIGGFLVGVFQRDMSLSFSASTFTILTIGDGLVGQIPALIIATATGIVATRTTQNEEEDFASKLITQLTNKSKTLVIVGAILLLFATIPGLPTFSLAFVGTLFLFIAWLISREGKDGLLTKLENYLSQKFGLDLSEKPHSSKIKPHTPTTRAKTQEELKREEEQAIDEVLKIEFLELALGYQLISLADMKQGGDLLERIRGIRKKIASDYGFLMPQIRIRDNLQLPPTHYEIKLKGIVIGEGMVMPDKFLAMNTGFVNKEIEGIPTKEPAFGMDALWIETKNKEEAIIQGYTIIDPSTVIATHTSELVKKYAEDFITKDEVKSLLERLAKDYPTIVEESKKIPTGAIRSVLQALLHEKIPIKDMLTILETITDIAPLVQNDVNILTEQVRARLSRVITNAFKSEDGRLKFLTFSTDSEQFLLNKLRENGTSKSLLLNVGELQKLIEVVSEEAMKVLQKGIAPVILIVEPNLRKALSNQMEQARIDVIVLSHAELDPNSNFEALGTIHINF flha-o25747 1335975 n/a 1335977 unspecified role unspecified role prey prey ecolx Escherichia coli not-defined-1850629092 1335978 unspecified role unspecified role bait bait region O06758-351-732 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 6 flgm-o25747 1335976 n/a 1335977 unspecified role unspecified role bait bait not-defined-1499542585 1335978 unspecified role unspecified role prey prey region O06758-351-732 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 3