UniProt UniProt http://www.uniprot.org teran-2009-1 Dpr Acts as a molecular switch, inhibiting Wnt signaling when unphosphorylated, but promoting Wnt signaling when phosphorylated by casein kinase Idelta/epsilon. in vitro In vitro in gel kinase assay in-gel kinase assay in gel kinase assay predetermined predetermined participant predetermined Teran E., Branscomb A.D., Seeling J.M. joni.seeling@qc.cuny.edu PLoS ONE 2009 mimix curation teran-2009-2 Dpr Acts as a molecular switch, inhibiting Wnt signaling when unphosphorylated, but promoting Wnt signaling when phosphorylated by casein kinase Idelta/epsilon. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Teran E., Branscomb A.D., Seeling J.M. joni.seeling@qc.cuny.edu PLoS ONE 2009 mimix curation dct1b_xenla Dapper 1-B Dapper1a dact1-b protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MKPIPAAPEPLGQHQDSPRRKDKGEAESERQRTRERLEATLAGLAELGHLRHRQEVLIKSVLSPGTRTHGDAAARTGDNPRSLEEKFLEDNILLLKKQLNCLRKRDAGLLSQLHELDKQINDLRIDVEKTEEHLETDSRPSSGFYELSDGTSGSLSNSSNSVFSECLSSCHSSTCFCNPLETSLNLTDGQAKSADDFLEWLDYRESQHETGTVRRSFSAPHSNSVDIEADVHPKYQCDLVSKNGNDIYRYPSPLHAVAVQSPMFLLSVMGNIKAEEPEEGIDHNDNDDCIVPELDHLKDEDSFLHQSSLCSLPLSSAKKMDGYILSIIQKKAHPVRTNKPRTSVNADPGKGILRHGSMCVKQTGGVSQSNAVNLKNSKQTCLHSTGMIAVDNSTYPSLKQCSKESLSEQLESKRMPSISTYPSCNVNELQSQNNSRNTVKSVCQGLARGSVAMTSNVQKENVTPNALANLSNTSSSVCNVTPGESMQNSPLLPQEIKVVPPVKRVSPQNTLLSYHASSSFDERPPLDFKSEGSSSQSLDEGLLVNAHYIPAQQQGVKLHKHTKYVKIVKSSTLKHRANVQYVAENGSQTLKEKSKVVGKKCRFPDDLDTNKKVKKSTLRVKKTAHPHFEPAVVGRNPVAVRSGSKSHGHSKDVVLAKPKHKRGDYRRWKSSAEISYEEALRRARRRAQGEMVGVYAQVPFPYSSPYAYIASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSLSEVEFVGESTTSSDTDESGGLIWSQFVQTLPMQATATAELQTTAKAFVKIKASHNLKKKILRFRSGSLKLMTTV dvl2_xenla Segment polarity protein dishevelled homolog DVL-2 dvl2 dsh DSH homolog 2 Xdsh protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAETKVIYHLDEEETPYLVKVPVPATDIRLRDFKAALGRGHAKYFFKAMDQDFGVVKEEISDDNAKLPCFNDRVVSWLASSEGSQPDSAPPAPATEVRPEPPPPVPPPIPPPPAERTSGIGDSRPPSFHPNVSGSTEQLDQDNESVISMRRDRVRRRESSEQAGVGRGVNGRTERHLSGYESSSTLLTSEIETSICDSEEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDINFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQGYFTLPRNEPIHPIDPAAWVSHSAALSGSFPVYPGSASMSSMTSSTSVTETELSHALPPVSLFSLSVHTDLASVVKVMASPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFQDRREARKFASNLLKAGFIRHTVNKITFSEQCYYIFGDLTGCENYMTNLSLNDNDGSSGASDQDTLAPLPLPGASPWPLLPTFSYQYQAPHPYSTQPPAYHELSSYSYGMGSAGSQHSEGSRSSGSNRSDGGRGMQKDDRSGVAGVGGGDSKSGSGSESEYSTRSSIRRVGGGEAGPPSERSTSSRLPPHHPPSVHSYAAPGVPLSYNPMMLMMMPPPPLPPPGVCPPNSSVPPGAPPLVRDLASVPPELTATRQSFHMAMGNPSEFFVDVM kc1d_rat Casein kinase I isoform delta Csnk1d Hckid Tau-protein kinase CSNK1D protein protein rat Rattus norvegicus (Rat) Rat MELRVGNRYRLGRKIGSGSFGDIYLGTDIAAGEEVAIKLECVKTKHPQLHIESKIYKMMQGGVGIPTIRWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGMERERKVSMRLHRGAPVNVSSSDLTGRQDTSRMSTSQIPGRVASSGLQSVVHR csnk1d-dact1_b-1 1310543 n/a 1310545 enzyme target enzyme target substrate substrate neutral component neutral component rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1310546 ancillary ancillary neutral component neutral component n/a 1310547 enzyme enzyme neutral component neutral component region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro Rat CKIalpha lacking its autoinhibtory domain was used (cannot not be mapped to UniProt. phosphorylation phosphorylation reaction phosphorylation false false false 2A dact1_b-dvl2 1310544 n/a 1310545 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1310546 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4A CKI-delta