mpidb The Microbial Protein Interaction Database chintakayala-2009-1 Allosteric regulation of the primase (DnaG) activity by the clamp-loader (tau) in vitro. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chintakayala K., Machón C., Haroniti A., Larson M.A., Hinrichs S.H., Griep M.A., Soultanas P. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation chintakayala-2009-2 Allosteric regulation of the primase (DnaG) activity by the clamp-loader (tau) in vitro. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Chintakayala K., Machón C., Haroniti A., Larson M.A., Hinrichs S.H., Griep M.A., Soultanas P. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation q9x4c9_geose Replicative DNA helicase dnaB protein protein geose Geobacillus stearothermophilus Geobacillus stearothermophilus Bacillus stearothermophilus MSELFSERIPPQSIEAEQAVLGAVFLDPAALVPASEILIPEDFYRAAHQKIFHAMLRVADRGEPVDLVTVTAELAASEQLEEIGGVSYLSELADAVPTAANVEYYARIVEEKSVLRRLIRTATSIAQDGYTREDEIDVLLDEADRKIMEVSQRKHSGAFKNIKDILVQTYDNIEMLHNRDGEITGIPTGFTELDRMTSGFQRSDLIIVAARPSVGKTAFALNIAQNVATKTNENVAIFSLEMSAQQLVMRMLCAEGNINAQNLRTGKLTPEDWGKLTMAMGSLSNAGIYIDDTPSIRVSDIRAKCRRLKQESGLGMIVIDYLQLIQGSGRSKENRQQEVSEISRSLKALARELEVPVIALSQLSRSVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYNKDSENKNIIEIIIAKQRNGPVGTVQLAFIKEYNKFVNLERRFDEAQIPPGA dnag_geose DNA primase dnaG protein protein geose Geobacillus stearothermophilus Geobacillus stearothermophilus Bacillus stearothermophilus MGHRIPEETIEAIRRGVDIVDVIGEYVQLKRQGRNYFGLCPFHGEKTPSFSVSPEKQIFHCFGCGAGGNAFTFLMDIEGIPFVEAAKRLAAKAGVDLSVYELDVRGRDDGQTDEAKAMTEAHALLKRFYHHLLVHTKEGQAALDYLQARGWTKETIDRFEIGYAPDAPDAAAKLLESHSFSLPVMEKAGLLTKKEDGRYVGRFRNRIMFPIHDHRGETVGFSGRLLGEGHPKYVNSPETPVFRKGAILYHFHAARVPIRKRQEALLVEGFADVISAAQAGIDYAIATMGTSLTEEQARILRPCDTITICYDGDRAGIEAAWAAAEQLSALGCRVKVASLPNGLDPDEYIRVYGGERFAGEAGCRRPLVAFKMAYLRRGKNLQHEGERLRYIDEALREIGKLSSPVEQDYYLRQLAEEFSLSLSALHEQLSRSQRERTKPREAPDGETARPMLAKKLLPAFQNAERLLLAHMMRSRDVALVVQERIGGRFNIEEHRALAAYIYAFYEEGHEADPGALISRIPGELQPLASDVSLLLIADDVSEQELEDYIRHVLNRPKWLMLKVKEQEKTEAERRKDFLTAARIAKEMIEMKKMLSSS dpo3x_bacsu DNA polymerase III subunit gamma/tau dnaH BSU00190 dnaX protein protein bacsu Bacillus subtilis MSYQALYRVFRPQRFEDVVGQEHITKTLQNALLQKKFSHAYLFSGPRGTGKTSAAKIFAKAVNCEHAPVDEPCNECAACKGITNGSISDVIEIDAASNNGVDEIRDIRDKVKFAPSAVTYKVYIIDEVHMLSIGAFNALLKTLEEPPEHCIFILATTEPHKIPLTIISRCQRFDFKRITSQAIVGRMNKIVDAEQLQVEEGSLEIIASAADGGMRDALSLLDQAISFSGDILKVEDALLITGAVSQLYIGKLAKSLHDKNVSDALETLNELLQQGKDPAKLIEDMIFYFRDMLLYKTAPGLEGVLEKVKVDETFRELSEQIPAQALYEMIDILNKSHQEMKWTNHPRIFFEVAVVKICQTSHQSAADLPEVDMLMKKIQQLEQEVERLKTTGIKAAAESPKKEAPRVPKGGKSNYKAPVGRIHEILKEATRPDLDLLRNSWGKLLAHLKQQNKVSHAALLNDSEPVAAGSAAFVLKFKYEIHCKMVAEDNNGVRTNLEQILESMLGKRMDLIGVPEAQWGKIREEFLEDHQQENEGSNEPAEEDPLIAEAKKLVGADLIEIKD dnab-dnag-1 1335935 not-defined-687808737 1335937 unspecified role unspecified role neutral component neutral component not-defined-1635885113 1335938 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 1F dnab-dnag-1 1335935 n/a 1335939 unspecified role unspecified role neutral component neutral component not-defined-687808737 1335937 unspecified role unspecified role neutral component neutral component not-defined-1635885113 1335938 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 1B dnax-dnab 1335936 n/a 1335939 unspecified role unspecified role bait bait P09122:p.Ala550Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P09122:p.Asp510Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P09122:p.Gly492Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P09122:p.Trp519Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P09122:p.Phe473Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P09122:p.Cys483Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false n/a 1335937 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3