mpidb The Microbial Protein Interaction Database mauriello-2009-1 AglZ regulates adventurous (A-) motility in Myxococcus xanthus through its interaction with the cytoplasmic receptor, FrzCD. in vitro In vitro pull down pull down sequence tag sequence tag identification sequence tag Mauriello E.M., Nan B., Zusman D.R. It is not clear from where strain the cloned proteins originated so these have been mapped to the reference proteome. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation mauriello-2009-2 AglZ regulates adventurous (A-) motility in Myxococcus xanthus through its interaction with the cytoplasmic receptor, FrzCD. in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Mauriello E.M., Nan B., Zusman D.R. It is not clear from where strain the cloned proteins originated so these have been mapped to the reference proteome. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation mauriello-2009-3 AglZ regulates adventurous (A-) motility in Myxococcus xanthus through its interaction with the cytoplasmic receptor, FrzCD. myxxd Myxococcus xanthus (strain DK 1622) fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Mauriello E.M., Nan B., Zusman D.R. It is not clear from where strain the cloned proteins originated so these have been mapped to the reference proteome. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation aglz_myxxd Adventurous-gliding motility protein Z MXAN_2991 aglZ protein protein myxxd Myxococcus xanthus (strain DK 1622) MERRVLIVESEHDFALSMATVLKGAGYQTALAETAADAQRELEKRRPDLVVLRAELKDQSGFVLCGNIKKGKWGQNLKVLLLSSESGVDGLAQHRQTPQAADGYLAIPFEMGELAALSHGIVPPGTDDTGASLDAALNGTREAPPPMPPSLKAAAGGPPKLPKRERRSAMTEEDRAFLDRTFQSIADRKAELLAESRQLKRPPPRRELMGTPEGKIQILRDELKTREAQLARLSEIWNVRERELLSGEDRIHEKDVELQGLKMQVDDLLRRFNEAQQATIQKEREHGATVDDLLLQKFSAEKDLIEVVASKEKDINLLRREVSRAEEELSRRAGELEHGRNEYDKLEKHLGVVTLEFEVKEQKLQDTVLANEGEIARLTKRGDDFEAELNRTISERDQRFAELDGEIQALQERLQQTEQERDTTVRGLEARAARAEEHGTQADAEIHRLNAERDALEAKLSQQVADLEADLARTMGERDQLRLDKDAQEAELTQRIEERDAKLGTLERELSETIARNEHTEAELNANIQQQLERIGELEGEVEAVKTHLEDRENELTAELQALGQAKDELETDLNDRLQALSQAKDALEADLSRQLEELRSAKAELEADLTGQIQALTSQLEETQRQLDDSQRTGEQLSARVAQLEDTVSQRESTIESLQGDVAARDQRISELSGDLEATSQTLAQTQQTLAQTEQQLADTQNTLASTEGALAETRGELDATSQTLQQTQQTLAQTEGALAETRGELDATSQTLAQTQQTLAQTEQQLADTQNTLASTEGTLAETRGELEATSQTLQQTHAALEDTRGALQETSDTLAHTTRERDQRIAELADLGAAKDALEQELTGQIGHLRSELSETQGNYEAERAAHEKLAAESSAHIGDLTSERDGLRSELEATSQTLEQTHGQLAATRDALAREQHAHQESRKAAASTQTTLEGQLAEARAHGEDLGEHLTLTKHELGTRVAELTQLTATLAQTENTRAHLEERLHTLTEESQRREELLQNDLTQKGTELSDTLRKLTHVTQEKMRQAEVLNREVATRTEQLKAMEAKLQTQATEARRQAEGLGQQITGLNEQLEQGRKALAGREDQLRAAGAAQQKLTAERDGLAGQLQQAEARLQQQAQQANQERADAKRAADELAAKLAKTEQRITQFAQDAQTQATEADARAKDLQGQLSARAKKIQDLELAVENAQGAKSRAEKELNAKVAAAESKAHEASTRLAAAQKERKDLEARHAKEQEDLAAKQKAELERRDAIKAQEVARLQQSVQEKSKALKVAELELARYKSKSATTATPAKAAAKPAAAEDDELAVRTQLNQVIAPAAAAQAPAPAKKPAAKPAAQAPAKKAPAPAPAPPAALSDESEPTDRTLVIQLPTAKEDDDWTALVDELDK q1d118_myxxd MXAN_5512 protein protein myxxd Myxococcus xanthus (strain DK 1622) MRCYTAPDRVRRLSRHRDTPRSTKTMARTTQEQTQERHAFLLELFRQQPDISSKEALESFKDKFGGTINMKTFNQLREQAEDEAASAAAAAPAPAPEVEPEPEPEPEPEETEATVDDAASRLKAAAAPEAINGAAGAPAKKAKAKGNGPKNIFVDAPKEHLTFLEGIIQQFQEAGAANIRIDHATDRWMVVVVDSK q1d2u5_myxxd MXAN_4870 protein protein myxxd Myxococcus xanthus (strain DK 1622) MEIPEDSRTLAYQASNWGRRCPSPRKTMRRSLLVCLVLLATASAAQEKKAPRDADLGRKSATAVDKSLAGDITREKKKEEVAPALQYDQFRLGVELQVASKRREQIQSLKKIISLSPDPKEAPSLLFRLGEFYWEESKFFFFEANRKDDDLIQAMNRNDAAGQQRAKAEKAEFSAKQKEYGKLAVEQYTKIVQEYPNFERTDEVLFFLGQYLMEDGQDRKALVAFKRLVEKHPQSKFIPDAYFAFGEYYFNNSKGKRPELEKALVAYKKAAEFPESQVYAFALYKQGWCHYNMGDFESAKDKFKTVVLYGELAGANALEKDGGKGGGRSSLLREARTDYVRAFAHHGDVAQARAEFGKVATNPEDRFTMLKQLANLYYGDGKDREAAITYNGLIKEKPLSPEAPGFQGRIVDCILRMGNKERTVVQVRRLVKIMKEVEGSGVIKEDKDKKLLAEAKELSERTLSNLAVTWHNEGKKTRNEETFRYADAVYSDYLTLFPENPKAYDLRFFWAELLNDSLQNFDKAAANYTLVVLQDAKVLEAKDDKGKPKPGKPGKWLQNAAYNAVLAYDEVVKAGEARGEGKSEAVGSDITKKATIPTLKKALLDACERYLKYVPKGEKRVEIAFKAANIYYRHNHFDEAVLRFSEIALGYPEYKFENGERAAEISANLILDSYHLLQDYAKVNEWARRFYANDKLAVGKFRDDLAKLIEQSSFKLVSQLEEKKEFEKAAEAYLAFVKDFPQTEIADLALYNASVDYYKAKRLDKAIEVRKRLFAEYPRSKHVPDSIYANAEALEAIGDFEDAAATYEAYVRGYERSLGDKGNAKARGGGKKKRGAVDDNKPAVVQKWDESKAQVALFNAATYREGLGQVKAALRNREHYLTLWPRAKDADEIRLSIIDLTAKSGAAFKAIKMLEEYERDNMRSPSRFLAAEGRIVDLYKKMGKTRDVARMQKRIFEHFDQLPRRVQGSLEKPALATAAQAQFLSVDLDWNEYRRLKLYWGAPPSPDRFKASIQDKSRALQVVEKKYVQTVTLGAPEPAICALHRIGLAYDHFADRLINAPMPRGLDEESEQALRDEFANQAQPLKDKATEAFSATVAKSRELDVYNDCAAESLKLLRTTYQPDRYPDMPDEKVALKGRVQLIGGDLLANIQDVPPPAPKAADAQQARAETLQEDLTDLTAQLRSQTETQVDARPAATTNGATPAKQGGNDEEPEDFL q1d8s0_myxxd MXAN_2736 protein protein myxxd Myxococcus xanthus (strain DK 1622) MAQKKAKRANRDLEKTYPRKDFVAKLRRLADAIEEGKAFNIQVAGERLRIPADALFNIEHERGDGVDEVEFQLRWQRE q1d4v8_myxxd Histidine kinase MXAN_4140 frzE protein protein myxxd Myxococcus xanthus (strain DK 1622) MDTEALKKSLLKKFQEVTADRLQKIQLGVLDLEKETADQAAEDVARELHTMKGEARMLGLAAIGQLAHAAEDVLRAEREGKTATEVATDVLLRACDVLSDLNEDLSGANTGNPASEEMVRMLAEVSGQTPPAIAGARPVAPPPAPPPAPVAAPVVTPAAVAAPPAPVQAPVAPPPTQAPVAEPGAHAAAAAPHPAAAHGRDEEAPSAAKSAVADRSIRVNVEVLDALGLLAGDLLVESARGRLRSSETEALFERFSRLGDRFLRLAEEIDISNEVREQLDRVESDLHMLRDDAFRFVRRNDDGINTLHGNLAKMADHVAEARLVPLSTVFDAFPRAVREMSRTQGKEVDLVIENADIGVDRSMLGDVRDALVHLLRNSVDHGVESPDTRQQLGKPLNGRIRIRVRVDGDMLHIEVEDDGRGIDPERLRQAAISKRLINAVQAAALSEREAIELIFRPGFSTRDQVSELSGRGVGMDVVKRKVETLGGSVGVSSRIGRGSTITLRLPQSLALMKVLLVRLGDDVYGMPAADVEAVMRVKPDDRLEIFGTLAVRHRGKPTALVALGPLLGLNGGNRFDKPPAVVVRHGEDHAALVVDGFVDEREVAVKPCGGEFLKAAPFIAGTAALEDGRIAVLLHVPDIMAEVRRMARPVTQAPAAKRLRVLLVDDSPIARATEGALVKALGHSVEEAQDGEEAYVKVQNNTYDLILTDVQMPKLDGFSLARRLKSTPAVARIPVIILSSLASPEDKRRGLDAGADAYLVKGELGVEVLAQAIDRLT q1d4v7_myxxd MXAN_4141 frzCD protein protein myxxd Myxococcus xanthus (strain DK 1622) MSLDTPNEKPAGKARARKAPASKAGATNAASTSSSTKAITDTLLTVLSGNLQARVPKELVGESGVELAHLLNQVLDQFAASEHRKHVAAQEIDQALDALIGLVREGDLSRWNTTTEDPQLGPLLEGFGKVIETLRTFVREINEAALRLSSSANQVLAASTQHETSSTEQAAAIHETTATMEELKHASAQIAENAGSVARVAEETLGAARAGRGAIGEFIQAMQQIRSDGVAVADSIAKLSKRVERIGTVVEVIDEIADRSDLLALNAALEGSRAGEAGKGFSIVAAEMRRLAENVLDSTKEIKNLITEIREATAAAAGAAEASKSATESGEKLGAVAAQAVEGILAGVQETSDAARVINLATQQQRTATEQVVASMAEIEDVTRQTTQASKQATGAAAELTQLAGRLAELIKRFKAD q1d7g6_myxxd MXAN_3206 protein protein myxxd Myxococcus xanthus (strain DK 1622) MVMKQNPLSFHPRAKPAAIAAMAAGEQGKYWEYHAKLFANQKKLDDVSLEQYAKELGLNLDKWKAELGNPKFQDIITRDQALAGQLGASGTPAFFINGRFLSGAQPIANFQALIDEELVKAENLVKGGVPASQVYAKIIEKGSERAAPKAQPQQPAATVRKVEIPSDSPSFGPANAKVTIVEWSDFECPFCSRVGPTLSKIKESYAKDVRVVFRHQPLPFHPNAKLAAEASHAAHEQGKFWEYHDKLFANQKAMDRASLEKYAQELGLNVAKFKAALDSGKFKAKVEADMAAGNAVGANGTPTFFINGREFVGAQPFEAFKRVIDEEIGKADKLLAAGTKPEELYAKLNAENVANAPTAPAAAPGAPAEPPVQKVDVGNAPVKGDKNAPVTIVAFSDFECPFCSRVVPTLKQLEDQYGGKIKVAFKNQPLPFHANAKLAAAAALAANEQGKFWEYHDKLFANQRALDRASLEKYAQELGLNVDKFKAALDQGKFNAQIEADMAQASSVGASGTPTFFINGRTLVGAQPVDAFKRVIDEELKKLGGAVADSK q1d4v5_myxxd MXAN_4143 frzA protein protein myxxd Myxococcus xanthus (strain DK 1622) MAPDRALAAQARPEQEFFCFRVGDLRLGVPSENVLEVLRAGLLTPLPRTPSFIMGVTGHRGEVLPVLDLLRFLSKGEARIGPRTRLFVGVTGSYVAGVVADTVLGLRRIPVADILPPPLGGDAAAEHLLGVVQASGNQEAINLLNFSKLLQTARQRAVAR q1d4v6_myxxd MXAN_4142 frzB protein protein myxxd Myxococcus xanthus (strain DK 1622) MDLLFFDIGATLYGTDASQVLRIDRALPEDLTLAELGLPHRGNRAIVFDTPEGEAHLKVDAVHGVRSIPVNSLRRMPPTAGAAAYAVGVCLEEARTVLLIDLVETARTQGRH frzcd-aglz-1 1335800 n/a 1335803 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) n/a 1335804 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) n/a 1335805 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) n/a 1335806 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) not-defined-274831402 1335807 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) not-defined-1931490122 1335808 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false myxxd Myxococcus xanthus (strain DK 1622) n/a 1335809 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) n/a 1335810 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) n/a 1335811 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) association association false false false Table 1 frzcd-aglz-2 1335800 n/a 1335803 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) n/a 1335809 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) n/a 1335805 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) n/a 1335806 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) not-defined-1931490122 1335808 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false myxxd Myxococcus xanthus (strain DK 1622) n/a 1335804 unspecified role unspecified role prey prey myxxd Myxococcus xanthus (strain DK 1622) association association false false false Table 1 aglz-frzcd 1335801 not-defined-1312714689 1335803 unspecified role unspecified role neutral component neutral component region Q1D823-2-240 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli not-defined-972899273 1335808 unspecified role unspecified role neutral component neutral component region Q1D4V7-2-135 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli 1335837 1335834 direct interaction direct interaction false false false Fig. 2 aglz-frzcd-1 1335802 n/a 1335803 unspecified role unspecified role neutral component neutral component region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1128357553 1335808 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false q1d4v7-2-280 Q1D4V7-2-280 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Fig. 7