UniProt UniProt http://www.uniprot.org shen-2009a-2 Arabidopsis protein kinases GRIK1 and GRIK2 specifically activate SnRK1 by phosphorylating its activation loop. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Shen W., Reyes M.I., Hanley-Bowdoin L. wshen@ncsu.edu Plant Physiol. (0032-0889) 2009 Only protein-protein interactions imex curation shen-2009a-1 Arabidopsis protein kinases GRIK1 and GRIK2 specifically activate SnRK1 by phosphorylating its activation loop. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shen W., Reyes M.I., Hanley-Bowdoin L. wshen@ncsu.edu Plant Physiol. (0032-0889) 2009 Only protein-protein interactions imex curation grik1_arath Serine/threonine-protein kinase GRIK1 Protein GEMINIVIRUS REP INTERACTING KINASE 1 SNAK2 SnRK1-activating protein kinase 2 F18N11 T14D3.180 At3g45240 GRIK1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MFCDSFAFAQVMSCFGCFGGSERSRHSPNPYDDDTYSHDSGETSNPGGDDEEGEEEEEVEELSRSKRSEEILKCKLQNGLVCRQFPVKETNKLTRGEDEDGNKTINEFVRERKIGSGSYGKVVLYRSTVDDKHYAIKAFHKSHLSRLRVAPSETAMGDVLREVMIMKTLEHPNIVNLIEVIDDPEFDDFYMVLEYVDGKWAYDDSGPPGALGEITARKYLRDVVAGLMYLHAHNVIHGDIKPDNLLVTSTGRVKIGDFSVSQVFKDDDDQLRRSPGTPVFTAPECCLGITYSGRSADTWAVGVTLYCMILGQYPFLGDTLQDTYDKIVHNPLIIPEGLNPRLRDLIEGLLCKDPNQRMTLKAVAEHPWITGEDGAISEYCCWCKRKAEEEEDQNHS q38997-2 SNF1-related protein kinase catalytic subunit alpha KIN10 KIN10 AK21 AKIN10 SKIN10 SNR2 SNRK1.1 T4P13.22 At3g01090 AKIN alpha-2 SNF1-related kinase 1.1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDGSGTGSRSGVESILPNYKLGRTLGIGSFGRVKIAEHALTGHKVAIKILNRRKIKNMEMEEKVRREIKILRLFMHPHIIRLYEVIETPTDIYLVMEYVNSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMVVHRDLKPENLLLDSKCNVKIADFGLSNIMRDGHFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYALLCGTLPFDDENIPNLFKKIKGGIYTLPSHLSPGARDLIPRMLVVDPMKRVTIPEIRQHPWFQAHLPRYLAVPPPDTVQQAKKIDEEILQEVINMGFDRNHLIESLRNRTQNDGTVTYYLILDNRFRASSGYLGAEFQETMEGTPRMHPAESVASPVSHRLPGLMEYQGVGLRSQYPVERKWALGLQSRAHPREIMTEVLKALQDLNVCWKKIGHYNMKCRWVPNSSADGMLSNSMHDNNYFGDESSIIENEAAVKSPNVVKFEIQLYKTRDDKYLLDLQRVQGPQFLFLDLCAAFLAQLRVL kin11_arath SNF1-related protein kinase catalytic subunit alpha KIN11 KIN11 AKIN alpha-1 AKIN11 SNF1-related kinase 1.2 SNR1 SNRK1.2 MXE2.16 At3g29160 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDHSSNRFGNNGVESILPNYKLGKTLGIGSFGKVKIAEHVVTGHKVAIKILNRRKIKNMEMEEKVRREIKILRLFMHPHIIRQYEVIETTSDIYVVMEYVKSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMVVHRDLKPENLLLDSRCNIKIADFGLSNVMRDGHFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYALLCGTLPFDDENIPNLFKKIKGGIYTLPSHLSSEARDLIPRMLIVDPVKRITIPEIRQHRWFQTHLPRYLAVSPPDTVEQAKKINEEIVQEVVNMGFDRNQVLESLRNRTQNDATVTYYLLLDNRFRVPSGYLESEFQETTDSGSNPMRTPEAGASPVGHWIPAHVDHYGLGARSQVPVDRKWALGLQSHAHPREIMNEVLKALQELNVCWKKIGHYNMKCRWVPGLADGQNTMVNNQLHFRDESSIIEDDCAMTSPTVIKFELQLYKAREEKYLLDIQRVNGPQFLFLDLCAAFLTELRVI grik2_arath Serine/threonine-protein kinase GRIK2 GRIK2 Protein GEMINIVIRUS REP INTERACTING KINASE 2 SNAK1 SnRK1-activating protein kinase 1 MUF9.18 At5g60550 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MFRDSFLFARTIGCFGCFGSSGSRNQQSPKPYDDDTHSCDSDVTSTARGEEEEDEEEVEQKSRSKRSEEILKYRLDNGLICRHIPVRETNELIRGEDENGDKTINEYVRVCKIGSGSYGKVVLYRSTLDGQYYAIKAFHKSHLLRLRVAPSETAMSDVLREVMIMKILEHPNIVNLIEVIDDPETDHFYMVLEYVDGKWVYDGSGPPGALGEKTARKYLRDIVTGLMYLHAHDVIHGDIKPDNLLVTSSGTVKIGDFSVSQVFKDDDDQLRRSPGTPVFTAPECCLVSGITYSGRAADTWAVGVTLYCMILGQYPFLADTLQDTYDKIVNNPLIIPDGLNPLLRDLIEGLLCKDPSQRMTLKNVSEHPWVIGEDGHVPEYFCWCKRNAASKIEEGEANGISETSDPN grik1-grik1-1 1331650 n/a 1331652 putative self putative self putative self putative self Q93V58:p.Lys137Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false true false Fig. 1A, FIg.4A grik1-kin10-1 1331650 n/a 1331652 enzyme enzyme unspecified role unspecified role Q93V58:p.Lys137Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1331653 enzyme target enzyme target substrate substrate unspecified role unspecified role kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain false Q38997-2:p.Thr175Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false Q38997-2:p.Lys48Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Fig. 1A, Fig. 4A grik1-kin11 1331650 n/a 1331654 enzyme target enzyme target substrate substrate unspecified role unspecified role kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P92958:p.Lys49Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1331652 enzyme enzyme unspecified role unspecified role Q93V58:p.Lys137Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Fig. 1A grik2-kin10 1331650 n/a 1331653 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q38997-2:p.Lys48Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false Q38997-2:p.Thr175Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1331655 enzyme enzyme unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q5HZ38:p.Lys136Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Fig. 1B, Fig. 4B grik2-kin11 1331650 n/a 1331654 enzyme target enzyme target substrate substrate unspecified role unspecified role P92958:p.Lys49Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1331655 enzyme enzyme unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q5HZ38:p.Lys136Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Fig. 1B snak1-snak1 1331650 n/a 1331655 putative self putative self putative self putative self region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q5HZ38:p.Lys136Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false true false Fig. 1B, Fig. 4B grik1-kin10 1331651 n/a 1331653 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1331652 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q93V58:p.Lys137Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false Fig. 3 grik2-kin10 1331651 n/a 1331653 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false kd activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1331655 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q5HZ38:p.Lys136Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false Fig. 3