mbinfo MBInfo http://www.mechanobio.info/ http://www.mechanobio.info/ li-2009b-1 Nuclear myosin II regulates the assembly of preinitiation complex for ICAM-1 gene transcription. human-circular smooth muscle Homo sapiens circular smooth muscle circular_smooth_muscle Circular smooth muscle anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li Q., Sarna S.K. Human colonic circular smooth muscle cells. The source of TFIIB, MHC, MLC20, alpha-actin and beta-actin (all endogenous) inferred from the cells (human colon smooth muscle) used. sksarna@utmb.edu Gastroenterology (0016-5085) 2009 Only protein-protein interactions imex curation li-2009b-2 Nuclear myosin II regulates the assembly of preinitiation complex for ICAM-1 gene transcription. human-circular smooth muscle Homo sapiens circular smooth muscle circular_smooth_muscle Circular smooth muscle confocal microscopy confocal microscopy immunostaining immunostaining Li Q., Sarna S.K. Human colonic circular smooth muscle cells. The source of TFIIB, MHC, MLC20, alpha-actin and beta-actin (all endogenous) inferred from the cells (human colon smooth muscle) used. sksarna@utmb.edu Gastroenterology (0016-5085) 2009 Only protein-protein interactions imex curation li-2009b-3 Nuclear myosin II regulates the assembly of preinitiation complex for ICAM-1 gene transcription. rat-muscularis externa Rattus norvegicus muscular_coat muscularis externa, tunica externa anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li Q., Sarna S.K. host: rat colon cells Human colonic circular smooth muscle cells. The source of TFIIB, MHC, MLC20, alpha-actin and beta-actin (all endogenous) inferred from the cells (human colon smooth muscle) used. sksarna@utmb.edu Gastroenterology (0016-5085) 2009 Only protein-protein interactions imex curation myh11_human Myosin-11 Myosin heavy chain 11 Myosin heavy chain, smooth muscle isoform SMMHC MYH11 KIAA0866 protein protein human Homo sapiens Human MAQKGQLSDDEKFLFVDKNFINSPVAQADWAAKRLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKHLPIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDTSITGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERTFHIFYYMIAGAKEKMRSDLLLEGFNNYTFLSNGFVPIPAAQDDEMFQETVEAMAIMGFSEEEQLSILKVVSSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLMGINVTDFTRSILTPRIKVGRDVVQKAQTKEQADFAVEALAKATYERLFRWILTRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCTEQGSHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFAKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAKEDELQKTKERQQKAENELKELEQKHSQLTEEKNLLQEQLQAETELYAEAEEMRVRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKIKKLEDEILVMDDQNNKLSKERKLLEERISDLTTNLAEEEEKAKNLTKLKNKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDDEIAQKNNALKKIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSHEAQVQEMRQKHAQAVEELTEQLEQFKRAKANLDKNKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEAQVQELQSKCSDGERARAELNDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLSSQLQDTQELLQEETRQKLNVSTKLRQLEEERNSLQDQLDEEMEAKQNLERHISTLNIQLSDSKKKLQDFASTVEALEEGKKRFQKEIENLTQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQRKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELEDARASRDEIFATAKENEKKAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNALQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATQQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLHEMEGAVKSKFKSTIAALEAKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEILLQVEDERKMAEQYKEQAEKGNARVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNETSFVPSRRSGGRRVIENADGSEEETDTRDADFNGTKASE ml12b_human Myosin regulatory light chain 12B MYL12B MRLC2 Myosin regulatory light chain 2-B, smooth muscle isoform MLC-2A SHUJUN-1 MYLC2B Myosin regulatory light chain MRLC2 Myosin regulatory light chain 20 kDa protein protein human Homo sapiens Human MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD tf2b_human Transcription initiation factor IIB GTF2B TF2B TFIIB General transcription factor TFIIB S300-II protein protein human Homo sapiens Human MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL actb_human Actin, cytoplasmic 1 ACTB Beta-actin protein protein human Homo sapiens Human MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF acta_human Actin, aortic smooth muscle Alpha-actin-2 Cell growth-inhibiting gene 46 protein ACTA2 ACTSA ACTVS GIG46 protein protein human Homo sapiens Human MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF tf2b_rat Transcription initiation factor IIB Gtf2b General transcription factor TFIIB RNA polymerase II alpha initiation factor protein protein rat Rattus norvegicus (Rat) Rat MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPSDFKFDTPVDKLPQL ml12b_rat Myosin regulatory light chain 12B Myl12b Mrlc2 Mrlcb Mylc2b Myosin RLC-B Myosin regulatory light chain 2-B, smooth muscle isoform Myosin regulatory light chain 20 kDa Myosin regulatory light chain MRLC2 protein protein rat Rattus norvegicus (Rat) Rat MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGRINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD myh11_rat Myosin-11 Myh11 Myosin heavy chain 11 Myosin heavy chain, smooth muscle isoform SMMHC protein protein rat Rattus norvegicus (Rat) Rat KKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSITQGPSFAYGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRHARDERTFHIFYYLIAGAKEKMRNDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFSEEEQLAILKVVSSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFAIEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTASKVIQRNCAAYLKLRNWQWWRLFTKVKPLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDLASTIEVMEEGKKRLQKEMEGLGQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMRTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAVKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEKKAKSLEAELMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATLQAEQLSNELVTERSAAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALEAKIVQLEEQIEQEAREKQAATKLLKQKDKKLKEVLLQVEDERKMVEQYKEQAEKGNTKVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNEASFVPSRRAGGRRVIENTDGSEEEMDARDSDFNGTKASE myh11-myl12b-1 1340768 n/a 1340771 unspecified role unspecified role bait bait n/a 1340772 unspecified role unspecified role prey prey physical association physical association false false false Fig 3A. myl12b-myh11 1340768 n/a 1340771 unspecified role unspecified role prey prey n/a 1340772 unspecified role unspecified role bait bait physical association physical association false false false Fig 3B. gtf2b-myh11 1340768 n/a 1340772 unspecified role unspecified role prey prey n/a 1340773 unspecified role unspecified role bait bait n/a 1340771 unspecified role unspecified role prey prey association association false false false Fig 3B. actb-myl12b-1 1340768 n/a 1340772 unspecified role unspecified role prey prey n/a 1340774 unspecified role unspecified role bait bait physical association physical association false false false Fig 3C. myl12b-acta2-1 1340768 n/a 1340775 unspecified role unspecified role prey prey n/a 1340774 unspecified role unspecified role prey prey n/a 1340772 unspecified role unspecified role bait bait association association false false false Fig 3D. acta2-myl12b 1340769 n/a 1340775 unspecified role unspecified role neutral component neutral component n/a 1340772 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 1D. actb-myl12b-2 1340769 n/a 1340772 unspecified role unspecified role neutral component neutral component n/a 1340774 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 1H. gtf2b-myh11-1 1340770 n/a 1340776 unspecified role unspecified role bait bait n/a 1340777 unspecified role unspecified role prey prey n/a 1340778 unspecified role unspecified role prey prey association association false false false Fig 6C. TBNS-trinitrobenzene sulfonic acid, which induces inflammation.