mpidb The Microbial Protein Interaction Database jakubowski-2009-1 Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis. rhird Rhizobium radiobacter (Agrobacterium tumefaciens) Agrobacterium radiobacter Agrobacterium tumefaciens anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jakubowski S.J., Kerr J.E., Garza I., Krishnamoorthy V., Bayliss R., Waksman G., Christie P.J. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation vird4_rhird Protein VirD4 virD4 protein protein rhird Rhizobium radiobacter (Agrobacterium tumefaciens) Agrobacterium radiobacter Agrobacterium tumefaciens MNSSKTSPQRMTLSIVCSLAAGFCAASCYVTFRRGFNGEAIMTFDVFAFWYETPLYLGYASTVFWRGLSVVIFTSLAVLSSQLIISLRNQKHHGTARWAEIGEMQHAGYLQRYSRIKGPIFGKTCGPLWFGSYLTNGEQPHSLVVAPTRAGKGVGIVIPTLLTFKGSVIALDVKGELFELTSRARKASGDAVFKFSPLDSERKTHCYNPVLDIATLPPERQFTETRRLAANLITAKGKGAEGFIDGARDLFVAGILTCIERGTPTIGAVYDLFAQPGEKYKLFAQLAEESLNKEAQRIFDNMAGNDTKILTSYTSVLGDGGLNLWADPLIKAATSRSDFSVYDLRRKKTCIYLCVSPNDLEVLAPLMRLLFQQLVSILQRSLPGKDECHEVLFLLDEFKHLGKLEAIETAITTIAGYKGRFMFIIQSLSALTGTYDDAGKQNFLSNTGVQVFMATADDETPTYISKAIGEYTFKARSTSYSQASMFDHNIQISDQGAALLRPEQVRLLDDQSEIVLIKGRPPLKLRKVQYYSDRTLKRLFERQMGSLPEPAPLMLSDYSNDQVQSHLAEIANFNEDAAPRNRTVAEDHGSVKVGADIPERVMGINGDEDQADAREIPPELGCASRIDAGSGRSTAIVGPDYCTSAKIEVRTGTACEMKLNWVQFVSGGMLRFDKVYARSQLSLYEVSV virba_rhird Protein virB10 virB10 protein protein rhird Rhizobium radiobacter (Agrobacterium tumefaciens) Agrobacterium radiobacter Agrobacterium tumefaciens MNNDSQQAAHEVDASGSLVSDKHRRRLSGSQKLIVGGVVLALSLSLIWLGGRQKKVNDNASPSTLIAANTKPFHPAPIEVPPDTPAVQEAVQPTVPQPPRGEPERMSHGRKKHRFLHIAVAIKGSASAPVRATWAEDKKTSVTTTPCRMRSVRRERFVDTYETHRAAAQQGTLLPHPDFMVTQGTIIPCILQTAIDTNLAGYVKCVLPQDIRGTTNNIVLLDRGTTVVGEIQRGLQQGDERVFVLWDRAETPDHAMISLTSPSADELGRPGLPGSVDSHFWQRFSGAMLLSAVQGAFQAASTYAGSSGGGMSFNSFQNNGEQTTETALKATINIPPTLKKNQGDTVSIFVARDLDFFGVYQLRLTGGAARGRNRRS virb9_rhird Protein virB9 virB9 protein protein rhird Rhizobium radiobacter (Agrobacterium tumefaciens) Agrobacterium radiobacter Agrobacterium tumefaciens MTRKALFILACLFAAATGAEAEDTPMAGKLDPRMRYLAYNPDQVVRLSTAVGATLVVTFATNETVTSVAVSNSKDLAALPRGNYLFFKASQVLTPQPVIVLTASDSGMRRYVFSISSKTLSHLDKEQPDLYYSVQFAYPADDAAARRREAQQRAVVDRLHAEAQYQRKAEDLLDQPVTALGATDSNWHYVAQGDRSLLPLEVFDNGFTTVFHFPGNVRIPSIYTINPDGKEAVANYSVKGSDVEISSVSRGWRLRDGHTVLCIWNAAYDPVGQRPQTGTVRPDVKRVLKGAKG virb7_rhird Outer membrane lipoprotein virB7 virB7 protein protein rhird Rhizobium radiobacter (Agrobacterium tumefaciens) Agrobacterium radiobacter Agrobacterium tumefaciens MKYCLLCLVVALSGCQTNDTIASCKGPIFPLNVGRWQPTPSDLQLRNSGGRYDGA virb10-vird4 1334943 n/a 1334944 unspecified role unspecified role prey prey n/a 1334945 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2A virb10-virb7 1334943 n/a 1334946 unspecified role unspecified role prey prey n/a 1334945 unspecified role unspecified role bait bait n/a 1334947 unspecified role unspecified role prey prey association association false false false Experiments were carried out under non-reducing conditions for co-precipitation of the VirB7–VirB9 dimer complex Fig. 2, 5B