mpidb The Microbial Protein Interaction Database o_hare-2008-1 Regulation of glutamate metabolism by protein kinases in mycobacteria. in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag O'Hare H.M., Durán R., Cerveñansky C., Bellinzoni M., Wehenkel A.M., Pritsch O., Obal G., Baumgartner J., Vialaret J., Johnsson K., Alzari P.M. hmo7@le.ac.uk Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation o_hare-2008-2 Regulation of glutamate metabolism by protein kinases in mycobacteria. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined O'Hare H.M., Durán R., Cerveñansky C., Bellinzoni M., Wehenkel A.M., Pritsch O., Obal G., Baumgartner J., Vialaret J., Johnsson K., Alzari P.M. hmo7@le.ac.uk Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation o_hare-2008-3 Regulation of glutamate metabolism by protein kinases in mycobacteria. in vitro In vitro pull down pull down sequence tag sequence tag identification sequence tag O'Hare H.M., Durán R., Cerveñansky C., Bellinzoni M., Wehenkel A.M., Pritsch O., Obal G., Baumgartner J., Vialaret J., Johnsson K., Alzari P.M. hmo7@le.ac.uk Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation o_hare-2008-4 Regulation of glutamate metabolism by protein kinases in mycobacteria. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined O'Hare H.M., Durán R., Cerveñansky C., Bellinzoni M., Wehenkel A.M., Pritsch O., Obal G., Baumgartner J., Vialaret J., Johnsson K., Alzari P.M. hmo7@le.ac.uk Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation gara_myctu Glycogen accumulation regulator GarA MTCY1A11.16c Rv1827 garA protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTDMNPDIEKDQTSDEVTVETTSVFRADFLSELDAPAQAGTESAVSGVEGLPPGSALLVVKRGPNAGSRFLLDQAITSAGRHPDSDIFLDDVTVSRRHAEFRLENNEFNVVDVGSLNGTYVNREPVDSAVLANGDEVQIGKFRLVFLTGPKQGEDDGSTGGP pkng_myctu Serine/threonine-protein kinase PknG MTCY22G10.06c Rv0410c pknG protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MAKASETERSGPGTQPADAQTATSATVRPLSTQAVFRPDFGDEDNFPHPTLGPDTEPQDRMATTSRVRPPVRRLGGGLVEIPRAPDIDPLEALMTNPVVPESKRFCWNCGRPVGRSDSETKGASEGWCPYCGSPYSFLPQLNPGDIVAGQYEVKGCIAHGGLGWIYLALDRNVNGRPVVLKGLVHSGDAEAQAMAMAERQFLAEVVHPSIVQIFNFVEHTDRHGDPVGYIVMEYVGGQSLKRSKGQKLPVAEAIAYLLEILPALSYLHSIGLVYNDLKPENIMLTEEQLKLIDLGAVSRINSFGYLYGTPGFQAPEIVRTGPTVATDIYTVGRTLAALTLDLPTRNGRYVDGLPEDDPVLKTYDSYGRLLRRAIDPDPRQRFTTAEEMSAQLTGVLREVVAQDTGVPRPGLSTIFSPSRSTFGVDLLVAHTDVYLDGQVHAEKLTANEIVTALSVPLVDPTDVAASVLQATVLSQPVQTLDSLRAARHGALDADGVDFSESVELPLMEVRALLDLGDVAKATRKLDDLAERVGWRWRLVWYRAVAELLTGDYDSATKHFTEVLDTFPGELAPKLALAATAELAGNTDEHKFYQTVWSTNDGVISAAFGLARARSAEGDRVGAVRTLDEVPPTSRHFTTARLTSAVTLLSGRSTSEVTEEQIRDAARRVEALPPTEPRVLQIRALVLGGALDWLKDNKASTNHILGFPFTSHGLRLGVEASLRSLARVAPTQRHRYTLVDMANKVRPTSTF dhe2_mycs2 NAD-specific glutamate dehydrogenase MSMEI_4582 NAD(+)-dependent glutamate dehydrogenase gdh MSMEG_4699 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MIRRLSVAFLSTYRGPQADAPGVTSTGPLAVAAHDDLVSDDLVAAHYRLASMRAPGETKAAVYPGDAGSGAALQIVTDQAPMLVDSVTVLLHRHGIAYTAIMNPVFRVRRGLDGELLDVRPAAEAAPGDGADECWILVPITAAADGEALTEATRLVPGILAEARQIGLDSGAMIAALHGLANDLATDLEGHFPNAERKEVAALLRWLADGHFVLLGYQQCVVGDGNAEVDPASRLGVLRLRNDVLPPLTDSDDLLVLAQATMPSYLRYGAYPYIVVVRESPGASRVIEHRFVGLFTVAAMNANALEIPLISRRVEEALAMAHRDPSHPGQLLRDIIQTIPRPELFALSSKQLLEMALAVVDLGSRRRTLLFLRADHLAHFVSCLVYLPRDRYTTAVRLEMQDILVRELGGAGIDYSARVSESPWAVVHFTVRLPEGTAADSVDTSLENESRIQDLLTEATRNWGDRMISAAAAASISPAALEHYAHAFPEDYKQAFAPQDAIADISLIEALQDDSVKLVLADTAEDRVWKLTWYLGGHSASLSELLPMLQSMGVVVLEERPFTLRRTDGLPVWIYQFKISPHPSIPHAPDAEAQRDTAQRFADAVTAIWHGRVEIDRFNELVMRAGLTWQQVVVLRAYAKYLRQAGFPYSQSHIESVLNENPHTTRSLIDLFEALFDPSQETDGRRDAQGAAAAVAADIDALVSLDTDRVLRAFANLIEATLRTNYFVARPDSARARNVLAFKLNPLVIKELPLPRPKFEIFVYSPRVEGVHLRFGFVARGGLRWSDRREDFRTEILGLVKAQAVKNAVIVPVGAKGGFVVKRPPTLTGDAAADREATRAEGVECYRLFISGLLDVTDNVDKATGAVVTPPEVVRRDGEDAYLVVAADKGTATFSDIANEVAKSYGFWLGDAFASGGSIGYDHKAMGITAKGAWESVKRHFREMGVDTQTQDFTVVGIGDMSGDVFGNGMLLSKHIRLVAAFDHRDIFLDPNPDAGRSWDERKRLFDLPRSSWADYDKSLISEGGGVYSRQQKSIPISPQVRTALGLDADVEELTPPALIKAILKAPVDLLWNGGIGTYIKAETEADADVGDRANDQIRVCGNQVRAKVIGEGGNLGVTALGRIEFDLAGGRINTDALDNSAGVDCSDHEVNIKILIDSAVTAGKVTPEERTELLLSMTDEVGELVLADNRDQNDLMGTSRANAASLLSVHARMIKDLVDNRGLNRELEALPSEKEIRRRADAGIGLTSPELATLMAHVKLALKDDVLASDLPDQEVFASRLPYYFPTRLREELHGEIRSHQLRREIITTMLVNDLVDTAGISYAYRITEDVGVGPVDAVRSYVAINAIFGIGDVWRRIRAAGDAGVPTSVTDRMTLDLRRLVDRAGRWLLNYRPQPLAVGAEINRFGAKVAALTPRMSEWLRGDDKAIVSKEAGDFASHGVPEDLAYHIATGLYQYSLLDVIDIADIVDREPDEVADTYFALMDHLGADALLTAVSRLSRDDRWHSLARLAIRDDIYGSLRALCFDVLAVGEPDENGEEKIAEWETTNSSRVTRARRTLTEIYKDGEQDLATLSVAARQIRSMTRTSGTGTTG dhe2_myctu NAD-specific glutamate dehydrogenase gdh NAD(+)-dependent glutamate dehydrogenase Rv2476c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTIDPGAKQDVEAWTTFTASADIPDWISKAYIDSYRGPRDDSSEATKAAEASWLPASLLTPAMLGAHYRLGRHRAAGESCVAVYRADDPAGFGPALQVVAEHGGMLMDSVTVLLHRLGIAYAAILTPVFDVHRSPTGELLRIEPKAEGTSPHLGEAWMHVALSPAVDHKGLAEVERLLPKVLADVQRVATDATALIATLSELAGEVESNAGGRFSAPDRQDVGELLRWLGDGNFLLLGYQRCRVADGMVYGEGSSGMGVLRGRTGSRPRLTDDDKLLVLAQARVGSYLRYGAYPYAIAVREYVDGSVVEHRFVGLFSVAAMNADVLEIPTISRRVREALAMAESDPSHPGQLLLDVIQTVPRPELFTLSAQRLLTMARAVVDLGSQRQALLFLRADRLQYFVSCLVYMPRDRYTTAVRMQFEDILVREFGGTRLEFTARVSESPWALMHFMVRLPEVGVAGEGAAAPPVDVSEANRIRIQGLLTEAARTWADRLIGAAAAAGSVGQADAMHYAAAFSEAYKQAVTPADAIGDIAVITELTDDSVKLVFSERDEQGVAQLTWFLGGRTASLSQLLPMLQSMGVVVLEERPFSVTRPDGLPVWIYQFKISPHPTIPLAPTVAERAATAHRFAEAVTAIWHGRVEIDRFNELVMRAGLTWQQVVLLRAYAKYLRQAGFPYSQSYIESVLNEHPATVRSLVDLFEALFVPVPSGSASNRDAQAAAAAVAADIDALVSLDTDRILRAFASLVQATLRTNYFVTRQGSARCRDVLALKLNAQLIDELPLPRPRYEIFVYSPRVEGVHLRFGPVARGGLRWSDRRDDFRTEILGLVKAQAVKNAVIVPVGAKGGFVVKRPPLPTGDPAADRDATRAEGVACYQLFISGLLDVTDNVDHATASVNPPPEVVRRDGDDAYLVVAADKGTATFSDIANDVAKSYGFWLGDAFASGGSVGYDHKAMGITARGAWEAVKRHFREIGIDTQTQDFTVVGIGDMSGDVFGNGMLLSKHIRLIAAFDHRHIFLDPNPDAAVSWAERRRMFELPRSSWSDYDRSLISEGGGVYSREQKAIPLSAQVRAVLGIDGSVDGGAAEMAPPNLIRAILRAPVDLLFNGGIGTYIKAESESDADVGDRANDPVRVNANQVRAKVIGEGGNLGVTALGRVEFDLSGGRINTDALDNSAGVDCSDHEVNIKILIDSLVSAGTVKADERTQLLESMTDEVAQLVLADNEDQNDLMGTSRANAASLLPVHAMQIKYLVAERGVNRELEALPSEKEIARRSEAGIGLTSPELATLMAHVKLGLKEEVLATELPDQDVFASRLPRYFPTALRERFTPEIRSHQLRREIVTTMLINDLVDTAGITYAFRIAEDVGVTPIDAVRTYVATDAIFGVGHIWRRIRAANLPIALSDRLTLDTRRLIDRAGRWLLNYRPQPLAVGAEINRFAAMVKALTPRMSEWLRGDDKAIVEKTAAEFASQGVPEDLAYRVSTGLYRYSLLDIIDIADIADIDAAEVADTYFALMDRLGTDGLLTAVSQLPRHDRWHSLARLAIRDDIYGALRSLCFDVLAVGEPGESSEQKIAEWEHLSASRVARARRTLDDIRASGQKDLATLSVAARQIRRMTRTSGRGISG pknb_myctu Serine/threonine-protein kinase PknB pknB MTCY10H4.14c Rv0014c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTTPSHLSDRYELGEILGFGGMSEVHLARDLRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAALNHPAIVAVYDTGEAETPAGPLPYIVMEYVDGVTLRDIVHTEGPMTPKRAIEVIADACQALNFSHQNGIIHRDVKPANIMISATNAVKVMDFGIARAIADSGNSVTQTAAVIGTAQYLSPEQARGDSVDARSDVYSLGCVLYEVLTGEPPFTGDSPVSVAYQHVREDPIPPSARHEGLSADLDAVVLKALAKNPENRYQTAAEMRADLVRVHNGEPPEAPKVLTDAERTSLLSSAAGNLSGPRTDPLPRQDLDDTDRDRSIGSVGRWVAVVAVLAVLTVVVTIAINTFGGITRDVQVPDVRGQSSADAIATLQNRGFKIRTLQKPDSTIPPDHVIGTDPAANTSVSAGDEITVNVSTGPEQREIPDVSTLTYAEAVKKLTAAGFGRFKQANSPSTPELVGKVIGTNPPANQTSAITNVVIIIVGSGPATKDIPDVAGQTVDVAQKNLNVYGFTKFSQASVDSPRPAGEVTGTNPPAGTTVPVDSVIELQVSKGNQFVMPDLSGMFWVDAEPRLRALGWTGMLDKGADVDAGGSQHNRVVYQNPPAGTGVNRDGIITLRFGQ 6C27EEBE9D5A453B kgd_myctu Multifunctional 2-oxoglutarate metabolism enzyme kgd 2-hydroxy-3-oxoadipate synthase 2-oxoglutarate carboxy-lyase 2-oxoglutarate decarboxylase Alpha-ketoglutarate decarboxylase Alpha-ketoglutarate-glyoxylate carboligase Rv1248c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MANISSPFGQNEWLVEEMYRKFRDDPSSVDPSWHEFLVDYSPEPTSQPAAEPTRVTSPLVAERAAAAAPQAPPKPADTAAAGNGVVAALAAKTAVPPPAEGDEVAVLRGAAAAVVKNMSASLEVPTATSVRAVPAKLLIDNRIVINNQLKRTRGGKISFTHLLGYALVQAVKKFPNMNRHYTEVDGKPTAVTPAHTNLGLAIDLQGKDGKRSLVVAGIKRCETMRFAQFVTAYEDIVRRARDGKLTTEDFAGVTISLTNPGTIGTVHSVPRLMPGQGAIIGVGAMEYPAEFQGASEERIAELGIGKLITLTSTYDHRIIQGAESGDFLRTIHELLLSDGFWDEVFRELSIPYLPVRWSTDNPDSIVDKNARVMNLIAAYRNRGHLMADTDPLRLDKARFRSHPDLEVLTHGLTLWDLDRVFKVDGFAGAQYKKLRDVLGLLRDAYCRHIGVEYAHILDPEQKEWLEQRVETKHVKPTVAQQKYILSKLNAAEAFETFLQTKYVGQKRFSLEGAESVIPMMDAAIDQCAEHGLDEVVIGMPHRGRLNVLANIVGKPYSQIFTEFEGNLNPSQAHGSGDVKYHLGATGLYLQMFGDNDIQVSLTANPSHLEAVDPVLEGLVRAKQDLLDHGSIDSDGQRAFSVVPLMLHGDAAFAGQGVVAETLNLANLPGYRVGGTIHIIVNNQIGFTTAPEYSRSSEYCTDVAKMIGAPIFHVNGDDPEACVWVARLAVDFRQRFKKDVVIDMLCYRRRGHNEGDDPSMTNPYVYDVVDTKRGARKSYTEALIGRGDISMKEAEDALRDYQGQLERVFNEVRELEKHGVQPSESVESDQMIPAGLATAVDKSLLARIGDAFLALPNGFTAHPRVQPVLEKRREMAYEGKIDWAFGELLALGSLVAEGKLVRLSGQDSRRGTFSQRHSVLIDRHTGEEFTPLQLLATNSDGSPTGGKFLVYDSPLSEYAAVGFEYGYTVGNPDAVVLWEAQFGDFVNGAQSIIDEFISSGEAKWGQLSNVVLLLPHGHEGQGPDHTSARIERFLQLWAEGSMTIAMPSTPSNYFHLLRRHALDGIQRPLIVFTPKSMLRHKAAVSEIKDFTEIKFRSVLEEPTYEDGIGDRNKVSRILLTSGKLYYELAARKAKDNRNDLAIVRLEQLAPLPRRRLRETLDRYENVKEFFWVQEEPANQGAWPRFGLELPELLPDKLAGIKRISRRAMSAPSSGSSKVHAVEQQEILDEAFG pkng_mycs2 Serine/threonine-protein kinase PknG pknG MSMEG_0786 MSMEI_0770 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MTSPENPDLPDADDAYVDSGPGTQPASLEDLDMDSASTMRPMATQAVYRPEFDDTDGTSRGTVVTEAYDQVTMATRALSPMRRLGGGLVEIPRVPERDPLTALMTNPVVAESKRFCWNCGKPVGRSTPDGRALSEGWCPHCGSPYSFLPQLSPGDIVADQYEIKGCIAHGGLGWVYLAFDKNVNDRPVVLKGLVHSGDAEAQAIAMAERQFLAEVTHPGIVKIYNFVEHEDKHGNPVGYIVMEYVGGTSLKQARGAKLPVAEAIGYMLEILPALGYLHSIGLAYNDLKPENIMITEEQLKLIDLGAVSRLNSYGYLYGTPGYQAPEIVRTGPTVATDIYTVGRTLAALTLSLRTRRGRYVDGLPSDDPVLETYDSYHRLLRRAIDPDPRRRFTSAEEMSSQLLGVLREVVATDTGVPRPGLSTVFSPSRSTFGVDLLVAHTDVYVDGQVHSEKLTAQEIVRALPVPLVDRTDVGAPMLVASVLSEPVHTLDQLRAARHGALDTEGIDLNESVELPLMEVRALLDLGDVAKATRKLEDLAARVGWRWRLVWFKAVSEMLSADYDSATKHFTEVLDTLPGELAPKLALAATAELAGTADELKFYKTVWSTDNGVISAGFGLARAQSVAGERDMAVQTLDEVPPTSRHFTTARLTSAVTLLSGRSTSEITEQHIRDAARRVEALPDSEPRVLQIRALVLGTALDWLADNTASSNHILGFPFTEHGLKLGVEASLRALARIAPTQSHRYALVDLANSVRPMSTF gara_mycs2 Glycogen accumulation regulator GarA garA MSMEG_3647 MSMEI_3561 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MTDKDSNLGADQSEDVTVETTSVFRADFLNELDAPAAAGTEGAVSGVEGLPSGSALLVVKRGPNAGSRFLLDQPTTSAGRHPDSDIFLDDVTVSRRHAEFRLEGGEFQVVDVGSLNGTYVNREPVDSAVLANGDEVQIGKFRLVFLTGPKSDDSGSNA kgd_mycs2 Multifunctional 2-oxoglutarate metabolism enzyme sucA MSMEG_5049 MSMEI_4922 2-hydroxy-3-oxoadipate synthase 2-oxoglutarate carboxy-lyase 2-oxoglutarate decarboxylase Alpha-ketoglutarate decarboxylase Alpha-ketoglutarate-glyoxylate carboligase kgd protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSSSPSPFGQNEWLVEEMYRKFRDDPSSVDPSWHEFLVDYSPEPTTDSASNGRTTTAAPVTPPTPAPAPAPEPKAAPKPAAKTEAKPAKPAKSATPAKGDESQILRGAAAAVVKNMNASLEVPTATSVRAIPAKLMIDNRVVINNHLKRTRGGKISFTHLLGYAIVQAVKKFPNMNRHFAVVDGKPTAITPAHTNLGLAIDLQGKDGNRSLVVAAIKRCETMRFGQFIAAYEDIVRRARDGKLTAEDFSGVTISLTNPGTLGTVHSVPRLMQGQGAIIGAGAMEYPAEFQGASEERIADLGIGKLITLTSTYDHRIIQGAESGDFLRTIHQLLLDDDFFDEIFRELGIPYEPVRWRTDNPDSIEDKNARVIELIAAYRNRGHLMADIDPLRLDNTRFRSHPDLDVNSHGLTLWDLDREFKVDGFAGVQRKKLRDILSVLRDAYCRHVGVEYTHILEPEQQRWIQERVETKHDKPTVAEQKYILSKLNAAEAFETFLQTKYVGQKRFSLEGAETVIPMMDAVIDQCAEHGLDEVVIAMPHRGRLNVLANIVGKPYSQIFSEFEGNLNPSQAHGSGDVKYHLGATGTYIQMFGDNDIEVSLTANPSHLEAVDPVLEGLVRAKQDLLDTGEEGSDNRFSVVPLMLHGDAAFAGQGVVAETLNLALLRGYRTGGTIHIVVNNQIGFTTAPTDSRSSEYCTDVAKMIGAPIFHVNGDDPEACAWVARLAVDFRQAFKKDVVIDMLCYRRRGHNEGDDPSMTQPYMYDVIDTKRGSRKAYTEALIGRGDISMKEAEDALRDYQGQLERVFNEVRELEKHEIEPSESVEADQQIPSKLATAVDKAMLQRIGDAHLALPEGFTVHPRVRPVLEKRREMAYEGRIDWAFAELLALGSLIAEGKLVRLSGQDTQRGTFTQRHAVIVDRKTGEEFTPLQLLATNPDGTPTGGKFLVYNSALSEFAAVGFEYGYSVGNPDAMVLWEAQFGDFVNGAQSIIDEFISSGEAKWGQLSDVVLLLPHGHEGQGPDHTSGRIERFLQLWAEGSMTIAMPSTPANYFHLLRRHGKDGIQRPLIVFTPKSMLRNKAAVSDIRDFTESKFRSVLEEPMYTDGEGDRNKVTRLLLTSGKIYYELAARKAKENREDVAIVRIEQLAPLPRRRLAETLDRYPNVKEKFWVQEEPANQGAWPSFGLTLPEILPDHFTGLKRISRRAMSAPSSGSSKVHAVEQQEILDTAFG gara-pkng 1334699 not-defined-1824619456 1334703 enzyme target enzyme target substrate substrate neutral component neutral component ecolx Escherichia coli not-defined-1270704157 1334704 enzyme enzyme neutral component neutral component ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false Fig. 2B Phosphorylation Thr-21 gara-gdh 1334699 not-defined-267829170 1334703 unspecified role unspecified role neutral component neutral component not-defined-1263962686 1334705 unspecified role unspecified role neutral component neutral component physical association physical association false false false gara-gdh-1 1334699 not-defined-945207285 1334706 unspecified role unspecified role neutral component neutral component not-defined-723141463 1334703 unspecified role unspecified role neutral component neutral component physical association physical association false false false gara-pknb 1334699 n/a 1334707 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli n/a 1334703 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false Fig. 2B Phosphorylation Thr-22 gara-pkng-2 1334700 not-defined-1824619456 1334703 unspecified role unspecified role bait bait ecolx Escherichia coli not-defined-1270704157 1334704 unspecified role unspecified role prey prey region nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false Fig. 3 Phosphorylation Thr-21 gara-gdh-7 1334700 n/a 1334708 unspecified role unspecified role prey prey ecolx Escherichia coli not-defined-525832318 1334703 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false Fig. 5c gara-gdh-8 1334700 n/a 1334706 unspecified role unspecified role prey prey ecolx Escherichia coli not-defined-525832318 1334703 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli association association false false false Fig. 5c gara-gdh-5 1334701 n/a 1334706 unspecified role unspecified role prey prey myctx Mycobacterium tuberculosis not-defined-525832318 1334703 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli n/a 1334708 unspecified role unspecified role prey prey myctx Mycobacterium tuberculosis n/a 1334704 unspecified role unspecified role prey prey myctx Mycobacterium tuberculosis association association false false false Fig. 5, Table 2 gara-gdh-6 1334701 n/a 1334709 unspecified role unspecified role prey prey mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) n/a 1334710 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false A0QYG2:p.Arg95Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli n/a 1334705 unspecified role unspecified role prey prey mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) n/a 1334711 unspecified role unspecified role prey prey mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) association association false false false Fig. 5 gara-pkng-3 1334702 not-defined-1824619456 1334703 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) not-defined-1270704157 1334704 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli 1334753 1334751 phosphorylation phosphorylation reaction phosphorylation false false false Supp. Figs. 1 & 2 Phosphorylation Thr-21