mpidb The Microbial Protein Interaction Database bingle-2008-1 A single aromatic residue in transcriptional repressor protein KorA is critical for cooperativity with its co-regulator KorB. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Bingle L.E., Rajasekar K.V., Muntaha S., Nadella V., Hyde E.I., Thomas C.M. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation bingle-2008-2 A single aromatic residue in transcriptional repressor protein KorA is critical for cooperativity with its co-regulator KorB. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Bingle L.E., Rajasekar K.V., Muntaha S., Nadella V., Hyde E.I., Thomas C.M. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation kora2_ecolx TrfB transcriptional repressor protein Regulatory protein KorA korA trfB protein protein ecolx Escherichia coli MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLGLTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR korb2_ecolx Transcriptional repressor protein KorB korB protein protein ecolx Escherichia coli MTAAQAKTTKKNTAAAAQEAAGAAQPSGLGLDSIGDLSSLLDAPAASQGGSGPIELDLDLIDEDPHQPRTADNPGFSPESIAEIGATIKERGVKSPISVRENQEQPGRYIINHGARRYRGSKWAGKKSIPAFIDNDYNEADQVIENLQRNELTPREIADFIGRELAKGKKKGDIAKEIGKSPAFITQHVTLLDLPEKIADAFNTGRVRDVTVVNELVTAFKKRPEEVEAWLDDDTQEITRGTVKLLREFLDEKGRDPNTVDAFNGQTDAERDAEAGDGQDGEDGDQDGKDAKEKGAKEPDPDKLKKAIVQVEHDERPARLILNRRPPAEGYAWLKYEDDGQEFEANLADVKLVALIEG korb-trfb 1334681 not-defined-1347411282 1334683 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-666660488 1334684 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Fig. 2 korb-trfb-2 1334682 not-defined-240361058 1334684 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-1210679171 1334683 unspecified role unspecified role prey prey P03052:p.Gln82Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P03052:p.Val74Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P03052:p.Tyr84Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P03052:p.Pro68Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P03052:p.Leu78Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false Fig. 4