mpidb The Microbial Protein Interaction Database botteaux-2008-2 Spa32 interaction with the inner-membrane Spa40 component of the type III secretion system of Shigella flexneri is required for the control of the needle length by a molecular tape measure mechanism. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Botteaux A., Sani M., Kayath C.A., Boekema E.J., Allaoui A. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation botteaux-2008-1 Spa32 interaction with the inner-membrane Spa40 component of the type III secretion system of Shigella flexneri is required for the control of the needle length by a molecular tape measure mechanism. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Botteaux A., Sani M., Kayath C.A., Boekema E.J., Allaoui A. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation span_shifl Surface presentation of antigens protein SpaN spaN spa32 CP0151 Spa32 protein protein protein shifl Shigella flexneri MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEYSLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQDIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHSLSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSEMTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKGNKNNSTGYNEQSEEEC q6xvw1_shifl spa40 protein protein shifl Shigella flexneri MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISFFSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKFVLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIFSQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKREYIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPFISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVWLEQVENTH q54046_saler spaN protein protein salmonella choleraes Salmonella choleraesuis MGDVSAVSSSGNILLPQQDEVGGLSEALKKAVEKHKTEYSDDKKAREYGDAFMMHKETALPVLLAAWRHGVPAKSEHHNGNVAGLHHNGKGELRIAEKLLKVTAEKSVGLISAEAKVDKSAALLSSKNGQLEGVSGKKLSADLKAVESVSELGDKATGISDDNLKALPGDNKAIAGEGVRKEGALLARDVAPSRMAAANTSKPDDKDHKKIKDVSQLPLQPTTIADLSQLSGGDEKMPLAAQSKPMMTIFPTVDGVKGEDSSLTYRFQRWGNDYSVNIQARQVGEFSLIPSNTQVEHRLHDQWQNGNPQRWHLTRDDQQNPQQQQHRQQSGEEDDA a1ju84_yere8 yscP YEP0026 protein protein yere8 Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) MNKITTRSPLEPEYQPLGKPHHDLQARVDFEQALLHNKDNRHPKEEPRRPVRPHDLGKKEGQKGDGLRAHAPLAATFQPGREVGLKPQHNHQNNHDFNLSPLAEGATNRKHLYQQDSRFDDRVESIINALMPLAPFLEGVTCETGTSSESPCEPFGHDELFVQQSPIDSAQPVQLNTKPTVQSLNPAADGAEVMIWSVGRDTLASIAKNQRDSRQKRLAEEPLPLHQEALPEVCPPAVSTTPDDHLVARWCATPVAEVAEKSARFPHKATVQSEQLDMTELADRSQHLTDGVDSSKDTIEPPRPEELLLPREETLPEMYSLSFTAPVITPGDHLLATMRATRLASVSEQLIQLAQRLAVELELRGGSSQVTQLHLNLPELGAIMVRIAEIPGKLHVELIASRETLRILAQGSYDLLERLQRIEPTQLDFQASGDSEQESRQKRHVYEEWEAEE spa40-span 1334651 not-defined-412452059 1334653 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-1736423857 1334654 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false shifl Shigella flexneri 1334662 1334659 physical association physical association false false false Fig. 4 spa40-span-3 1334651 not-defined-395093744 1334654 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-114509984 1334655 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli physical association physical association false false false Fig. 6E spa40-yscp 1334651 not-defined-772061279 1334654 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-153418985 1334656 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 6D spa40-span-2 1334652 not-defined-412452059 1334653 unspecified role unspecified role prey prey ecolx Escherichia coli not-defined-1736423857 1334654 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false shifl Shigella flexneri physical association physical association false false false Fig. 4