mpidb The Microbial Protein Interaction Database el_qaidi-2009-1 Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism. ecolx Escherichia coli adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined El Qaidi S., Allemand F., Oberto J., Plumbridge J. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation el_qaidi-2009-2 Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps tag fluorescence tag visualisation by fluorescence tag fluorescence El Qaidi S., Allemand F., Oberto J., Plumbridge J. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation gals_ecoli HTH-type transcriptional regulator GalS Mgl repressor and galactose ultrainduction factor galS b2151 JW2138 protein protein ecoli Escherichia coli (strain K12) MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGAGTPDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM galr_ecoli HTH-type transcriptional regulator GalR Galactose operon repressor galR b2837 JW2805 protein protein ecoli Escherichia coli (strain K12) MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDDRYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGEPDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSLEASHHATSD galr-gals 1334560 not-defined-1528165637 1334562 unspecified role unspecified role prey prey not-defined-1938238660 1334563 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5A gals-galr 1334560 not-defined-719876248 1334562 unspecified role unspecified role bait bait not-defined-1878743015 1334563 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5A galr-galr 1334560 n/a 1334563 unspecified role unspecified role prey prey not-defined-1938238660 1334563 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5A gals-gals 1334560 not-defined-719876248 1334562 unspecified role unspecified role bait bait n/a 1334562 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5A galr-gals-1 1334561 not-defined-1878743015 1334563 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal alexa 488 label alexa 488 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli not-defined-719876248 1334562 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false 1334561 Fig. 5B galr-galr-1 1334561 n/a 1334563 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-1938238660 1334563 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal alexa 488 label alexa 488 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false Fig. 5B