mpidb The Microbial Protein Interaction Database hua-2009-2 Mutagenesis of region 4 of sigma 28 from Chlamydia trachomatis defines determinants for protein-protein and protein-DNA interactions. ecolx Escherichia coli 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hua Z., Rao X., Feng X., Luo X., Liang Y., Shen L. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation hua-2009-1 Mutagenesis of region 4 of sigma 28 from Chlamydia trachomatis defines determinants for protein-protein and protein-DNA interactions. saltm Salmonella typhimurium anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hua Z., Rao X., Feng X., Luo X., Liang Y., Shen L. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation o84064_chltr fliA CT_061 protein protein chltr chltr MKTHDLADTWQLYWSTKEIHHRDVLIESYLPLVKNVAHRLASGMPSHVKMEDLYALGVEGLIRAVERFDPEKSKRFESYALFIIKAAIIDGLRKQDWVPRSVYQRANRLADAMDSLRQTLGKEPTDGELCEYLNISQQELSHWFSSSRPALVLSLHDDFSCQDDDEGLALEERIADERAETGYDVIRKKEAISILTEALLALDEKERQVMALYYYDDLVLKEIGKILGVSESRVSQIHSKALLKLRGTLSSLL rpob_chltr DNA-directed RNA polymerase subunit beta rpoB RNA polymerase subunit beta Transcriptase subunit beta CT_315 protein protein chltr chltr MFKCPERVSIKKKEDILDLPNLVEVQIKSYKQFLQIGKLAEERENIGLEEVFREIFPIKSYNEATILEYLSYNLGVPKYSPEECIRRGITYSVTLKVRFRLTDETGIKEEEVYMGTIPIMTDKGTFIINGAERVVVSQVHRSPGINFEQEKHSKGNVLFSFRIIPYRGSWLEAVFDINDLIYIHIDRKKRRRKILAMTFIRALGYSTDADIIEEFFSVEERSLRLEKDFVALVGKVLADNVVDADSSLVYGKAGEKLSTAMLKRILDAGVQSLKIAVGADENHPIIKMLAKDPTDSYEAALKDFYRRLRPGEPATLVNARSTIMRLFFDAKRYNLGRVGRYKLNKKLGFPLDDETLSQVTLRKEDVIGALKYLIRLRMGDEKTSIDDIDHLANRRVRSVGELIQNHCRSGLARMEKIVRERMNLFDFSSDTLTPGKIISAKGLVSVLKDFFSRSQLSQFMDQTNPVAELTHKRRLSALGPGGLNRERAGFEVRDVHASHYGRICPIETPEGPNIGLITSLSSFAKINEFGFIETPYRVVRDGIVTDEIEYMTADVEEECVIAQASAELDEYDMFKTPVCWARYKGEAFEADTSTVTHMDVSPKQLVSVVTGLIPFLEHDDANRALMGSNMQRQAVPLLKTEAAIVGTGLEGRAAKDSGAIIVAQEDGVVEYVDSYEIVVAKKNNPTLKDRYQLKKFLRSNSGTCINQTPLCSVGDVVTHGDVLADGPATDKGELALGKNVLVAFMPWYGYNFEDAIIISERLIKQDAYTSIYIEEFELTARDTKLGKEEITRDIPNVSEEVLANLGEDGVVRIGAEVKPGDILVGKITPKSETELAPEERLLRAIFGEKAADVKDASLTVPPGTEGVVMDVKVFSRKDRLSKSDDELVEEAVHLKDLQKEYKSQLAQLKVEHREKLGALLLNEKAPAAIIHRRSADILVQEGAIFDQETIELLERESLVDLLMAPCDMYDVLKDILSSYETAVQRLEVNYKTEAEHIKEGDADLDHGVIRQVKVYVASKRKLQVGDKMAGRHGNKGVVSKIVPEADMPFLANGETVQMILNPLGVPSRMNLGQVLETHLGYAAKTAGIYVKTPVFEGFPESRIWDMMIEQGLPEDGKSYLFDGKTGERFDSKVVVGYIYMLKLSHLIADKIHARSIGPYSLVTQQPLGGKAQMGGQRFGEMEVWALEAYGVAHMLQEILTVKSDDVSGRTRIYESIVKGENLLRSGTPESFNVLIKEMQGLGLDVRPMVVDA rpob_ecoli DNA-directed RNA polymerase subunit beta rpoB groN nitB rif ron stl stv tabD b3987 Transcriptase subunit beta RNA polymerase subunit beta JW3950 protein protein ecoli Escherichia coli (strain K12) MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNVLLKEIRSLGINIELEDE c1k5f0_saltm DNA-directed RNA polymerase subunit beta rpoB DRM14_23415 A3W75_20915 H8S97_21190 STMLT2P22_CBEKMEGD_00318 CVR97_00095 JJB80_20880 JJB81_20875 A3X55_22595 RNA polymerase subunit beta Transcriptase subunit beta A4O05_20000 A4O41_22335 A6D61_23535 A9U69_22085 AAB79_22190 AAC35_22680 AF497_21035 AGM99_22860 AHN93_23385 AL144_21200 AL168_20990 AL184_21375 AQ530_23495 AVC05_19380 AXX99_22400 B1265_23725 B1398_15840 B1P38_23725 B2E31_22655 B4V59_22125 B6362_22575 B7Q27_22550 B8Y16_22325 B9C90_21395 B9C96_21380 B9M14_23025 B9O84_22200 BBQ66_22125 BIC00_22440 BIC13_21985 BK110_22315 BKM50_23240 BMS46_22230 BMU56_22360 BZ203_22720 BZZ88_23190 C5W43_23940 CA117_22240 CB102_22435 CB198_22350 CB570_21420 CB646_22690 CBZ90_23985 CC339_21000 CC403_22250 CC453_18215 CC652_22980 CC971_22400 CCP17_23005 CDZ72_23425 CE70_23870 CED07_23115 CEQ70_24600 CFF58_23845 CFF59_22990 CIX60_24445 CPS79_22880 CQO33_23555 CSG22_23730 D4361_23780 D4387_23355 D4422_23055 D5N86_23520 D5N95_23330 D5O82_23115 D5P17_23755 D5X47_23860 D5Y28_23095 D6J79_24140 D8S24_23360 DLB93_23765 DLR28_23170 DMO92_23485 DN165_23235 DNB97_24455 DNM27_22825 DNZ37_24105 DPB42_23480 DPD91_22840 DPF41_22870 DPF68_23225 DPS76_22730 DQD22_23840 DQR44_24135 DRR75_24205 DRT38_23970 DRT61_23650 DRV05_22910 DSF94_23190 DTF68_23730 DU071_23950 DU657_23015 DU879_23340 E1A11_22990 E6W45_22650 EBD14_23355 EBK21_23370 EC404_23390 EEQ30_23455 EER35_20805 EL822_23015 EPB30_23105 EQG93_22865 ERL99_22930 F3Q97_22320 F3R12_22600 F3R39_22925 F9G02_21730 F9O44_18825 FEM52_23065 FGZ46_21525 GCZ80_23375 GEZ01_22685 KP44_23210 NG06_18650 R035_20890 Z700_21720 ZY40_24335 G4F88_21915 G4F89_22235 G4F91_21890 G4F92_22035 G0040_23775 G0A05_23860 G0A32_22845 G0A39_22425 G0A43_21585 G0A46_23705 G0A50_22465 G0A51_22890 G0A52_22470 G0A53_22425 G0A58_22735 G0A60_22970 G0A61_21990 G0A63_22400 G0A67_22645 G0A68_22010 G0A70_23400 G0A73_22960 G0A76_22230 G0A96_23965 G0A97_22080 G0B03_23205 G0B05_24160 G0B07_23995 G0B12_23365 G0B96_24045 G0C04_24950 G0E20_23455 G0G84_19105 G0J24_23185 G0J26_22795 G0J27_14680 G0J28_23250 G0J31_22885 G0J33_22295 G0J34_23525 G0J36_23280 G0J37_22715 G0J40_23335 G0J43_23230 G0J44_23215 G0J45_23575 G0J46_23395 G0J47_23110 G0J49_23360 G0J50_22880 G0J51_23030 G0J55_23160 G0J59_22980 G0J62_19375 G0J65_24135 G0J73_23090 G0J79_23630 G0J82_22620 G0J89_23455 G0J92_22790 G0J94_23440 G0J96_23595 G0J97_23260 G0K00_23380 G0K02_23415 G0K03_23205 G0K05_19070 G0K10_24855 G0K15_23555 G0K16_23705 G0K18_22735 G0K19_24220 G0K20_21880 G0K23_23175 G0K28_23680 G0K32_22675 G0K38_23030 G0K39_23205 G0K42_23155 G0K47_22835 G0K48_23235 G0K49_23180 G0K52_23160 G0K53_23705 G0K56_23035 G0K58_21695 G0K59_23325 G0K61_22800 G0K68_22645 G0K74_23915 G0K80_22510 G0K83_22975 G0K84_23085 G0K85_22785 G0K88_004600 G0K89_004586 G0K90_004565 G0K94_002284 G0K95_004728 G0L00_003475 G0L02_004743 G0L03_23970 G0L06_22940 G0L07_16770 G0L14_13855 G0L15_22945 G0L18_23450 G0L19_15210 G0L20_20440 G0L24_22070 G0L25_20685 G0L29_20705 G0L31_20155 G0L32_20885 G0L34_20455 G0L35_19780 G0L36_18345 G0L37_19890 G0L38_18840 G0L42_14280 G0L48_16245 G0L49_14945 G0L51_21710 G0L52_19925 G0L55_21595 G0L59_21980 G0L62_22385 G0L63_21905 G0L65_22015 G0L67_20445 G0L76_21210 G0L77_21865 G0L78_21930 G0L79_22225 G0L83_21930 G0L86_004394 G0L89_21545 G0L91_21905 G0L93_21335 G0L96_21910 G0L98_21020 G0M00_21695 G0M05_20575 G0M06_004303 G0M13_004392 G0M14_21790 G0M16_22005 G0M18_000951 G0M21_19390 G0M22_004310 G0M25_004354 G0M26_21955 G0M29_004365 G0M33_17450 G0M36_21285 G0M38_21030 G0M39_21885 G0M41_21885 G0M45_21365 G0M53_22750 G0M55_22925 G0M56_23055 G0M58_24225 G0M63_24120 G0M65_23010 G0M67_22595 G0N51_22240 G0N57_21410 G0N71_22525 G0N92_20930 G0O00_23640 G0O15_22730 G0O37_23460 G0O42_23430 G0O57_23865 G0O59_23435 G0O63_22470 G0O66_22910 G0O74_23770 G0O86_22135 G0O94_22960 G0P00_22980 G0P02_23160 G0P05_23825 G0P08_24600 G0P12_22830 G0P19_23395 G0P36_23610 G0P65_23340 G0P67_23195 G0P73_21180 G0P75_23875 G1N61_23880 G1N64_24415 G1N66_23860 G1N68_23880 G1N71_23905 G1N72_23405 G1N86_23345 G1N87_23510 G1N91_23920 G1O00_23905 G1O02_23510 G1O04_23480 G1O05_23820 G1O08_24225 G1O12_23450 G1O16_23900 G1O17_23560 G1O18_23510 G1O20_23900 G1O23_23670 G1O25_23495 G1O26_23890 G1O27_23565 G1O28_23530 G1O29_23935 G1O32_24465 G1O34_23570 G1O38_23895 G1O40_23510 G1O43_23495 G1O46_24455 G1O48_23815 G1O49_24540 G1O51_23885 G1O53_23970 G1O62_23835 G1O63_23890 G1O65_23905 G1O67_23540 G1O68_23500 G1O69_23865 G1O71_23595 G1O72_23575 G1O76_23980 G1O77_23900 G1O80_24000 G1O81_23890 G1O83_24720 G1O84_23985 G1O87_23590 G1O88_23580 G1O89_23505 G1O90_23925 G1O93_23970 G1O94_24195 G1O96_24820 G1P02_23565 G1P03_23870 G1P06_23830 G1P09_23855 G1P10_23910 G1P12_23605 G1P14_23135 G1P15_24050 G1P17_23520 G1P19_24170 G1P23_23595 G1P24_23505 G1P25_24310 G1P26_23870 G1P29_23825 G1P31_23905 G1P35_23910 G1P36_23895 G1P37_23855 G1P40_24605 G1P44_23445 G1P45_23980 G1P47_23965 G1P48_23875 G1P51_23590 G1P52_23810 G1P53_23955 G1P54_23920 G1P55_23895 G1P56_23955 G1P57_23555 G1P58_23810 G1P59_23580 G1P64_23580 G1P67_23565 G1P69_23450 G1P72_22505 G1P75_23290 G1P76_23495 G1P78_23690 G1P83_23715 G1P84_23910 G1P87_23595 G1P90_23495 G1P91_23855 G1Q03_23450 G1Q08_23790 G1Q67_23975 G1Q78_22840 G1Q81_23935 G1Q83_23575 G1Q84_22870 G1Q85_23495 G1Q86_23965 G1Q88_23565 G1Q90_23890 G1Q91_23700 G1Q93_23525 G1Q96_23750 G1Q98_23785 G1Q99_22995 G1R01_23905 G1R02_23740 G1R03_23970 G1R04_23510 G1R08_22825 G1R13_23485 G1R15_23100 G1R20_23905 G1R21_23550 G1R22_23905 G1R23_23670 G1R27_23670 G1R28_23900 G1R29_23525 G1R30_23615 G1R31_23465 G1R36_23910 G1R38_23710 G1R40_24255 G1R42_23335 G1R44_23900 G1R45_23530 G1R47_23625 G1R48_23135 G1R51_23540 G1R53_24020 G1R63_23580 G1R69_24000 G1R87_23870 G1R93_23420 G1S02_24125 G2212_22075 G2218_22965 G2279_24000 G2290_23690 G2793_22540 G2918_22475 G3221_004670 G3247_004239 G3254_004516 G3263_004888 G3270_004546 G3275_004454 G3312_003936 G3357_004733 G3369_004639 G3593_004679 G3A35_21755 G3V14_004693 G3V17_004642 G3V21_004099 G3V56_004446 G3V57_004670 G4189_004555 G4201_004273 G4202_004676 G4A73_004427 G4A85_004638 G4A87_004366 G4B68_004192 G4B72_004010 G4C74_004476 G4D46_004461 G4G47_004701 G4G62_004311 G4G67_004750 G4G68_004273 G4G75_004499 G4G76_004144 G4G97_004754 G4H00_004750 G4H07_004311 G4H08_004280 G4H18_004393 G4H21_004447 G4H24_004413 G4I66_004407 G4J08_004640 G4J11_004705 G4O54_004614 G4O56_004458 G4O67_004233 G4O69_004450 G4P29_004445 G4P83_004640 G4P85_004664 G4P89_004190 G4P93_004630 G4Q12_004325 G4Q28_004700 G4Q31_004406 G4Q50_004471 G4Q52_004476 G4Q59_004626 G4Q60_004772 G4Q63_004792 G4Q67_004139 G4Q94_004637 G4R01_004735 G4R02_004701 G4R15_004641 G4R16_004670 G4W68_004301 G4W73_004383 G4W86_004298 G4W87_004445 G4W88_004703 G4W91_004656 G4Y10_004455 G9302_004607 G9304_004441 G9305_004360 G9309_004286 G9313_004527 G9314_004273 G9367_004738 G9381_004253 G9C57_004440 G9C64_004735 G9G03_004497 G9G04_004556 G9W28_004564 G9W52_004310 G9W63_004464 G9W65_004528 G9W79_004379 G9W95_004451 G9W96_004651 GB021_22510 GB040_18820 GB106_22245 GB114_22525 GB122_23285 GB131_23050 GB209_22520 GB221_22140 GB238_22525 GB331_22880 GB339_22235 GB368_22655 GB372_22440 GB416_23510 GB452_19145 GB459_23145 GB466_24070 GB505_23155 GB510_18910 GB567_22420 GB645_21365 GBS44_23270 GBV53_23935 GBV54_23710 GBV60_22195 GBW03_21215 GBW44_22820 GBW76_22380 GBX12_21720 GBX20_23445 GBX46_23170 GBX55_23325 GBX64_22435 GBY13_23350 GBY23_23250 GBY73_22505 GBZ51_17310 GBZ55_22065 GNA88_004684 GNA97_004548 GNA99_004671 GNB28_004398 GNB36_004439 GNB86_004500 GNC11_004468 GNC19_004755 GNC45_004417 GNC75_004367 GT380_24295 GTH62_21715 GTH70_21950 GTH77_22875 GTH78_23510 GTH89_22485 GTH90_21710 GTH99_22760 GXC51_22830 GXC56_22745 GXG40_22775 GYI58_22715 GYI62_004500 GYI77_24705 GYJ27_21165 GYJ28_003569 GYJ53_23310 GYJ59_22315 GYJ60_23560 protein protein saltm Salmonella typhimurium MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVVFEIRDNKLQMELIPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDIKHIEVPVEYIAGKVVSKDYVDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETVRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLRDEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRRVVDGVVTDEIHYLSAIEEGNYVIAQANSNLDDEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGTVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLFSRIRAVLVSSGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDDEVLRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDLPTSGQITLFDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNVLLKEIRSLGINIELEDE rpob-flia-1 1334436 not-defined-167882498 1334438 unspecified role unspecified role prey prey region 4 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O84064:p.Glu206Gly O84064-206-206 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false O84064:p.Glu222Gly O84064-222-222 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false O84064:p.Gln236Leu O84064-236-236 mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false O84064:p.Tyr214Cys O84064-214-214 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false not-defined-2022710818 1334439 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1334450 1334444 physical association physical association false false false figure 5 rpob-flia-3 1334436 n/a 1334440 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-167882498 1334438 unspecified role unspecified role prey prey O84064:p.Tyr214Cys O84064-214-214 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false O84064:p.Glu206Gly O84064-206-206 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false O84064:p.Glu222Gly O84064-222-222 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region 4 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O84064:p.Gln236Leu O84064-236-236 mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false 1334458 1334453 physical association physical association false false false figure 5 rpob-flia-2 1334437 n/a 1334441 unspecified role unspecified role bait bait n/a 1334438 unspecified role unspecified role prey prey physical association physical association false false false figure 4