mpidb The Microbial Protein Interaction Database chintakayala-2008-1 Conserved residues of the C-terminal p16 domain of primase are involved in modulating the activity of the bacterial primosome. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Chintakayala K., Larson M.A., Griep M.A., Hinrichs S.H., Soultanas P. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation chintakayala-2008-2 Conserved residues of the C-terminal p16 domain of primase are involved in modulating the activity of the bacterial primosome. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc weight by comassie molecular weight estimation by coomasie staining weight by comassie Chintakayala K., Larson M.A., Griep M.A., Hinrichs S.H., Soultanas P. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation q9x4c9_geose Replicative DNA helicase dnaB protein protein geose Geobacillus stearothermophilus Geobacillus stearothermophilus Bacillus stearothermophilus MSELFSERIPPQSIEAEQAVLGAVFLDPAALVPASEILIPEDFYRAAHQKIFHAMLRVADRGEPVDLVTVTAELAASEQLEEIGGVSYLSELADAVPTAANVEYYARIVEEKSVLRRLIRTATSIAQDGYTREDEIDVLLDEADRKIMEVSQRKHSGAFKNIKDILVQTYDNIEMLHNRDGEITGIPTGFTELDRMTSGFQRSDLIIVAARPSVGKTAFALNIAQNVATKTNENVAIFSLEMSAQQLVMRMLCAEGNINAQNLRTGKLTPEDWGKLTMAMGSLSNAGIYIDDTPSIRVSDIRAKCRRLKQESGLGMIVIDYLQLIQGSGRSKENRQQEVSEISRSLKALARELEVPVIALSQLSRSVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYNKDSENKNIIEIIIAKQRNGPVGTVQLAFIKEYNKFVNLERRFDEAQIPPGA dnag_geose DNA primase dnaG protein protein geose Geobacillus stearothermophilus Geobacillus stearothermophilus Bacillus stearothermophilus MGHRIPEETIEAIRRGVDIVDVIGEYVQLKRQGRNYFGLCPFHGEKTPSFSVSPEKQIFHCFGCGAGGNAFTFLMDIEGIPFVEAAKRLAAKAGVDLSVYELDVRGRDDGQTDEAKAMTEAHALLKRFYHHLLVHTKEGQAALDYLQARGWTKETIDRFEIGYAPDAPDAAAKLLESHSFSLPVMEKAGLLTKKEDGRYVGRFRNRIMFPIHDHRGETVGFSGRLLGEGHPKYVNSPETPVFRKGAILYHFHAARVPIRKRQEALLVEGFADVISAAQAGIDYAIATMGTSLTEEQARILRPCDTITICYDGDRAGIEAAWAAAEQLSALGCRVKVASLPNGLDPDEYIRVYGGERFAGEAGCRRPLVAFKMAYLRRGKNLQHEGERLRYIDEALREIGKLSSPVEQDYYLRQLAEEFSLSLSALHEQLSRSQRERTKPREAPDGETARPMLAKKLLPAFQNAERLLLAHMMRSRDVALVVQERIGGRFNIEEHRALAAYIYAFYEEGHEADPGALISRIPGELQPLASDVSLLLIADDVSEQELEDYIRHVLNRPKWLMLKVKEQEKTEAERRKDFLTAARIAKEMIEMKKMLSSS dnab-dnab-2 1333346 not-defined-1792058456 1333348 enzyme enzyme neutral component neutral component ecolx Escherichia coli Stoichiometry: 6.0 direct interaction direct interaction false false false Fig. 5 dnab-dnag 1333346 not-defined-1792366004 1333349 enzyme enzyme neutral component neutral component Q9X4D0:p.Pro458Ala Q9X4D0-495-495 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false not-defined-2002919410 1333348 enzyme enzyme neutral component neutral component direct interaction direct interaction false false false Fig. 5 dnab-dnag-1 1333347 not-defined-2025921205 1333348 enzyme enzyme neutral component neutral component not-defined-364707915 1333349 enzyme enzyme neutral component neutral component Q9X4D0:p.Tyr548Ala Q9X4D0-548-548 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1333347 Fig. 6