mpidb The Microbial Protein Interaction Database korotkova-2008-1 Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Korotkova N., Yang Y., Le Trong I., Cota E., Demeler B., Marchant J., Thomas W.E., Stenkamp R.E., Moseley S.L., Matthews S. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation korotkova-2008-3 Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Korotkova N., Yang Y., Le Trong I., Cota E., Demeler B., Marchant J., Thomas W.E., Stenkamp R.E., Moseley S.L., Matthews S. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation korotkova-2008-2 Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Korotkova N., Yang Y., Le Trong I., Cota E., Demeler B., Marchant J., Thomas W.E., Stenkamp R.E., Moseley S.L., Matthews S. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation korotkova-2008-4 Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation. in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation predetermined predetermined participant predetermined Korotkova N., Yang Y., Le Trong I., Cota E., Demeler B., Marchant J., Thomas W.E., Stenkamp R.E., Moseley S.L., Matthews S. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation korotkova-2008-5 Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation. in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Korotkova N., Yang Y., Le Trong I., Cota E., Demeler B., Marchant J., Thomas W.E., Stenkamp R.E., Moseley S.L., Matthews S. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation korotkova-2008-6 Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Korotkova N., Yang Y., Le Trong I., Cota E., Demeler B., Marchant J., Thomas W.E., Stenkamp R.E., Moseley S.L., Matthews S. Mol. Microbiol. (0950-382X) 2008 Only protein-protein interactions imex curation ceam5_human Carcinoembryonic antigen-related cell adhesion molecule 5 CEACAM5 CEA Carcinoembryonic antigen Meconium antigen 100 protein protein human Homo sapiens Human MESPSAPPHRWCIPWQRLLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYTLHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWVNNQSLPVSPRLQLSNGNRTLTLFNVTRNDTASYKCETQNPVSARRSDSVILNVLYGPDAPTISPLNTSYRSGENLNLSCHAASNPPAQYSWFVNGTFQQSTQELFIPNITVNNSGSYTCQAHNSDTGLNRTTVTTITVYAEPPKPFITSNNSNPVEDEDAVALTCEPEIQNTTYLWWVNNQSLPVSPRLQLSNDNRTLTLLSVTRNDVGPYECGIQNKLSVDHSDPVILNVLYGPDDPTISPSYTYYRPGVNLSLSCHAASNPPAQYSWLIDGNIQQHTQELFISNITEKNSGLYTCQANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASGTSPGLSAGATVGIMIGVLVGVALI q7bg35_ecolx draE protein protein ecolx Escherichia coli MKKLAIMAAASMVFAVSSAHAGFTPSGTTGTTKLTVTEECQVRVGDLTVAKTRGQLTDAAPIGPVTVQALGCDARQVALKADTDNFEQGKFFLISDNNRDKLYVNIRPTDNSAWTTDNGVFYKNDVGSWGGIIGIYVDGQQTNTPPGNYTLTLTGGYWAK afae3_ecolx Afimbrial adhesin AFA-III afaE3 afaE-3 protein protein ecolx Escherichia coli MKKLAIMAAASMVFAVSSAHAGFTPSGTTGTTKLTVTEECQVRVGDLTVAKTRGQLTDAAPIGPVTVQALGCNARQVALKADTDNFEQGKFFLISDNNRDKLYVNIRPMDNSAWTTDNGVFYKNDVGSWGGTIGIYVDGQQTNTPPGNYTLTLTGGYWAK ceacam5-ceacam5-1 1332521 n/a 1332527 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Table1 1.95A Rworking = 0.18 Rfree = 0.2 ceacam5-ceacam5 1332521 n/a 1332527 unspecified role unspecified role neutral component neutral component P06731:p.Val73Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Table 1 2.9A Rworking = 0.22 Rfree = 0.25 ceacam5-ceacam5-2 1332522 n/a 1332527 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 n/a 1332527 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P06731:p.Val73Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false 3 ceacam5-drae 1332522 n/a 1332527 unspecified role unspecified role bait bait P06731:p.Val73Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 n/a 1332528 unspecified role unspecified role prey prey Stoichiometry: 2.0 direct interaction direct interaction false false false Fig4C-E DTT DraE appears to disrupt N-CEA dimers prior to binding to the resulting monomer. ceacam5-2 1332523 n/a 1332527 unspecified role unspecified role neutral component neutral component P06731:p.Gln78Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Gln78Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Ser66Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Tyr68Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Ile125Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P06731:p.Phe63Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Leu129Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Glu133Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Fig.1, Table 2 ceacam5-2 1332524 n/a 1332527 unspecified role unspecified role neutral component neutral component P06731:p.Phe63Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P06731:p.Leu129Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Gln78Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Ile125Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Tyr68Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Ser66Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P06731:p.Glu133Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false 1332524 Supp. Fig.1, Table 2 1.95A Rworking = 0.18 Rfree = 0.2 ceacam5-2 1332525 n/a 1332527 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P06731:p.Val73Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 4A afae3-ceacam5 1332526 n/a 1332527 unspecified role unspecified role neutral component neutral component P06731:p.Leu52Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false P06731:p.Leu54Thr mutation mutation certain certain sequence position certain certain certain sequence position certain false not-defined-898946582 1332529 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Table 4