mpidb The Microbial Protein Interaction Database bu-2008-2 Interaction between two putative glycosyltransferases is required for glycosylation of a serine-rich streptococcal adhesin. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Bu S., Li Y., Zhou M., Azadin P., Zeng M., Fives-Taylor P., Wu H. J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation bu-2008-1 Interaction between two putative glycosyltransferases is required for glycosylation of a serine-rich streptococcal adhesin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bu S., Li Y., Zhou M., Azadin P., Zeng M., Fives-Taylor P., Wu H. J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation bu-2008-3 Interaction between two putative glycosyltransferases is required for glycosylation of a serine-rich streptococcal adhesin. ecolx Escherichia coli pull down pull down sequence tag sequence tag identification sequence tag Bu S., Li Y., Zhou M., Azadin P., Zeng M., Fives-Taylor P., Wu H. J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation gtfa_strpa UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit gtfA Glycosyltransferase Gtf1 Glycosyltransferase GtfA gtf1 protein protein streptococcus parasa Streptococcus parasanguis MTIYNINLGIGWASSGVEYAQAYRAQILRSLGMPAKFIFTNMFQSENLEHFTKNIGFEDNEIIWLYGYFTDVKISGTTYKKDDLEATFSQCPTKKEASSDRKLIRYYFENQELYINASLYGENQEYVQRVEYVVKGKLIRKDYYSYTKVFSEFYSPGENGVQLCNRSFYNEDGSIAYEEILSNEKSTFVFSNKICYGLEELLEFMLEDLSLTKSDLILLDRATGIGQVVFENIGAAKLAVVIHAEHFNEKNTDEHNILWNNYYEYQFTNADKVNAFITSTERQKILLEEQFTQYTSLHPKIVAIPVGSLDQLKFPEQSRKSFSMMTGSRLAIEKHIDWLIEGVALAQKRLPELTFDIYGEGGERRKLTELLTKLHAGEFIELKGHKQLDEIYQNYELYLTASTSEGFGLTLMEAVGSGLPIIGFDVPYGNQTFVCSGENGLLIERPKGDDRSRIVQAFADSIYEYFTKFKMADAQQYSYNIAENYKHEKLVERWKDFIEEMLND gtfb_strpa UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase stabilizing protein GtfB gtfB Glycosyltransferase chaperone Gtf2 Glycosyltransferase stabilizing protein GtfB Glycosyltransferase-stabilizing protein Gtf2 gtf2 protein protein streptococcus parasa Streptococcus parasanguis MIRLFEWLTQESLDLHYSLEESGIHGTSIVLNDDGFLPEGIISPYTFFCEVEMDGSPLYFNQLEVPYLWQITGTNIEGEIWNRSSKRGVIHYHEPKYLRFVQSVDWLYPDGSIYMTDHYNKYGWAFARTYFFSDQQVSHKKYYTKSGQEVLSENILTGDILLNWKGKVYHFTKKVDFFLFYFKKSGLDLSSIWYNSLGMPFLISYYLGGEGRDILFWQENLADQLPGNMQIIFSGRTSRTKKVIVQDRSVYKKLLHLVEEKNKEMISFLNIIYPKLRENYSRKEILIVTNSDQIEGIETLTDNLSAYTFHIGALTSMSDKLQNIGQKENVLLYPNMSPKTMLDLLEQCDIYLDINHGNEVLSIVRLAFERSLLILAYDNTVHSPIFHHESGIFNHSKPQTLSDWLLNLDDYSQTVSCWRSDLFPMTYRDYKQVLVSNVD gtf1-gtf2-2 1332477 not-defined-1070679458 1332480 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-1360613804 1332481 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig. 4B gtf2-gtf1-1 1332477 not-defined-684213988 1332481 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-249373279 1332480 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig. 4B gtf1-gtf2-3 1332477 not-defined-1360613804 1332481 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind insertion analysis insertion analysis range range range range false region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro not-defined-1070679458 1332480 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli physical association physical association false false false Fig. 6C gtf1-gtf2 1332478 not-defined-1360613804 1332481 unspecified role unspecified role prey prey not-defined-1070679458 1332480 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4A gtf2-gtf1 1332478 not-defined-684213988 1332481 unspecified role unspecified role bait bait not-defined-249373279 1332480 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4A gtf1-gtf2-1 1332479 not-defined-1332033257 1332480 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-784582645 1332481 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5