UniProt UniProt http://www.uniprot.org jensen-2008-5 p68 (Ddx5) interacts with Runx2 and regulates osteoblast differentiation. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Jensen E.D., Niu L., Caretti G., Nicol S.M., Teplyuk N., Stein G.S., Sartorelli V., van Wijnen A.J., Fuller-Pace F.V., Westendorf J.J. Origin of Runx 2 according to following: mouse runx2 isoform used in GST- pull down fig 3e (deduced from seq length). Stated RT-PCR from mouse cells. For TAP-Runx2 stated they used a 'MRIPV isoform" which could correspond to human Q13950-2, or mouse Q08775-3 or Q08775-4 in UniProt. Consequently mouse origin was chosen. Origin of p68 constructs according pmid 10837141 (stated that constructs have been provided by Janknecht R. who used GST-p68 in pmid 12527917 stating there that the clone derived from Fuller-Pace F.V. (who worked with human, see 10837141 for cDNA cloning). westendorf.jennifer@mayo.edu J. Cell. Biochem. (0730-2312) 2008 Only protein-protein interactions imex curation jensen-2008-1 p68 (Ddx5) interacts with Runx2 and regulates osteoblast differentiation. rat-umr-106 Rattus norvegicus umr-106 Rat UMR-106 osteosarcoma cell line tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Jensen E.D., Niu L., Caretti G., Nicol S.M., Teplyuk N., Stein G.S., Sartorelli V., van Wijnen A.J., Fuller-Pace F.V., Westendorf J.J. Origin of Runx 2 according to following: mouse runx2 isoform used in GST- pull down fig 3e (deduced from seq length). Stated RT-PCR from mouse cells. For TAP-Runx2 stated they used a 'MRIPV isoform" which could correspond to human Q13950-2, or mouse Q08775-3 or Q08775-4 in UniProt. Consequently mouse origin was chosen. Origin of p68 constructs according pmid 10837141 (stated that constructs have been provided by Janknecht R. who used GST-p68 in pmid 12527917 stating there that the clone derived from Fuller-Pace F.V. (who worked with human, see 10837141 for cDNA cloning). westendorf.jennifer@mayo.edu J. Cell. Biochem. (0730-2312) 2008 Only protein-protein interactions imex curation jensen-2008-2 p68 (Ddx5) interacts with Runx2 and regulates osteoblast differentiation. rat-umr-106 Rattus norvegicus umr-106 Rat UMR-106 osteosarcoma cell line tap tandem affinity purification TAP TAP tap western blot western blot Immuno blot Immuno blot Jensen E.D., Niu L., Caretti G., Nicol S.M., Teplyuk N., Stein G.S., Sartorelli V., van Wijnen A.J., Fuller-Pace F.V., Westendorf J.J. Origin of Runx 2 according to following: mouse runx2 isoform used in GST- pull down fig 3e (deduced from seq length). Stated RT-PCR from mouse cells. For TAP-Runx2 stated they used a 'MRIPV isoform" which could correspond to human Q13950-2, or mouse Q08775-3 or Q08775-4 in UniProt. Consequently mouse origin was chosen. Origin of p68 constructs according pmid 10837141 (stated that constructs have been provided by Janknecht R. who used GST-p68 in pmid 12527917 stating there that the clone derived from Fuller-Pace F.V. (who worked with human, see 10837141 for cDNA cloning). westendorf.jennifer@mayo.edu J. Cell. Biochem. (0730-2312) 2008 Only protein-protein interactions imex curation jensen-2008-3 p68 (Ddx5) interacts with Runx2 and regulates osteoblast differentiation. mouse-c2c12 Mouse C3H muscle myoblast c2c12 Mouse C3H muscle myoblast confocal microscopy confocal microscopy immunostaining immunostaining Jensen E.D., Niu L., Caretti G., Nicol S.M., Teplyuk N., Stein G.S., Sartorelli V., van Wijnen A.J., Fuller-Pace F.V., Westendorf J.J. Origin of Runx 2 according to following: mouse runx2 isoform used in GST- pull down fig 3e (deduced from seq length). Stated RT-PCR from mouse cells. For TAP-Runx2 stated they used a 'MRIPV isoform" which could correspond to human Q13950-2, or mouse Q08775-3 or Q08775-4 in UniProt. Consequently mouse origin was chosen. Origin of p68 constructs according pmid 10837141 (stated that constructs have been provided by Janknecht R. who used GST-p68 in pmid 12527917 stating there that the clone derived from Fuller-Pace F.V. (who worked with human, see 10837141 for cDNA cloning). westendorf.jennifer@mayo.edu J. Cell. Biochem. (0730-2312) 2008 Only protein-protein interactions imex curation jensen-2008-4 p68 (Ddx5) interacts with Runx2 and regulates osteoblast differentiation. rat-ros17/2.8 Rattus norvegicus ROS17/2.8 osteosarcoma cell line ros17/2.8 ROS 17/2.8 osteosarcoma cell line confocal microscopy confocal microscopy immunostaining immunostaining Jensen E.D., Niu L., Caretti G., Nicol S.M., Teplyuk N., Stein G.S., Sartorelli V., van Wijnen A.J., Fuller-Pace F.V., Westendorf J.J. Origin of Runx 2 according to following: mouse runx2 isoform used in GST- pull down fig 3e (deduced from seq length). Stated RT-PCR from mouse cells. For TAP-Runx2 stated they used a 'MRIPV isoform" which could correspond to human Q13950-2, or mouse Q08775-3 or Q08775-4 in UniProt. Consequently mouse origin was chosen. Origin of p68 constructs according pmid 10837141 (stated that constructs have been provided by Janknecht R. who used GST-p68 in pmid 12527917 stating there that the clone derived from Fuller-Pace F.V. (who worked with human, see 10837141 for cDNA cloning). westendorf.jennifer@mayo.edu J. Cell. Biochem. (0730-2312) 2008 Only protein-protein interactions imex curation q08775-3 Runt-related transcription factor 2 PEBP2-alpha A1 Runx2 Aml3 Cbfa1 Osf2 Pebp2a Acute myeloid leukemia 3 protein Core-binding factor subunit alpha-1 Oncogene AML-3 Osteoblast-specific transcription factor 2 Polyomavirus enhancer-binding protein 2 alpha A subunit SL3-3 enhancer factor 1 alpha A subunit SL3/AKV core-binding factor alpha A subunit protein protein mouse Mus musculus Mouse MRIPVDPSTSRRFSPPSSSLQPGKMSDVSPVVAAQQQQQQQQQQQQQQQQQQQQQQQQQQQQEAAAAAAAAAAAAAAAAAAVPRLRPPHDNRTMVEIIADHPAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPREPRRHRQKLDDSKPSLFSDRLSDLGRIPHPSMRVGVPPQNPRPSLNSAPSPFNPQGQSQITDPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLPAITDVPRRISDDDTATSDFCLWPSSLSKKSQAGASELGPFSDPRQFPSISSLTESRFSNPRMHYPATFTYTPPVTSGMSLGMSATTHYHTYLPPPYPGSSQSQSGPFQTSSTPYLYYGTSSASYQFPMVPGGDRSPSRMVPPCTTTSNGSTLLNPNLPNQNDGVDADGSHSSSPTVLNSSGRMDESVWRPY ddx5_human Probable ATP-dependent RNA helicase DDX5 HLR1 DDX5 HELR G17P1 DEAD box protein 5 RNA helicase p68 protein protein human Homo sapiens Human MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ lima1_rat LIM domain and actin-binding protein 1 Epithelial protein lost in neoplasm Lima1 eplin protein protein rat Rattus norvegicus (Rat) Rat MESTPFNRRQWTSLSLRVTAKELSLVNKNKSSTIVEIFSKYQKAAEEANMERKKNNTESLPQHFRRGTLSVLKKKWENPVAGAESHTDSLPNSSSDGGHTADHPPAEVTAKAAPGARADREEHTQPRSRFGSRPEAVTQCRYPRSEDSHDFKAQATESQNMENCLGDSRHEAEKPEMSENTETSGKIEKYNVPLNRLKMMFEKGEHSQNKSPWTQGRNAGGRRLSENSCSLDDLEIGAGHLSSSAFNSEKNESKRNLELPRLSETSIKDRMAKYQAAVSKQSSPASYASELKPSESKTHKWEQKENVPPGPEACSIHQEGSKVSATENSLVAHPVPAEDDTCNSQGRSEAQQPIYTKPLSPDARTSSLPESSPSKTAKKFQAPARESCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHKQHKDLWASKGENEETLGRPAQPPSAGETPHSPGVEDAPIAKVGVLAASMEAKASSQREREENKPAETKKLRIAWPPPAEQGSSGSAPEEGFKVSKPKWPPEDEVCKTEAPEDVDLDLKKLRRSSSLKERSRPFTVAASFRTSSVKSPKPLSPSLRKGWSEPEPEQSEEFGGGTVTQTESPRPSREKESVGKSRWQSEEAEAEAEEAPRGRDGRSFELESESFIGNGASIAEDDVAPAQRSPLEPESPGWPGFGDTTTAKEFNQKSQDVGFWEGEVVRELSVEEQIKRNRYYDEDEDEE gels_rat Gelsolin Gsn Actin-depolymerizing factor Brevin protein protein rat Rattus norvegicus (Rat) Rat MAPYCSSLRSALLVLALCALSPSHAATASRGRAQERAPQSRVSETRPSTMVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPPNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESSTFQGYFKSGLKYKKGGVASGFKHVVPNEVVVQRLFQVKGRRVVRATEVPVSWDSFNNGDCFILDLGNNIYQWCGSGSNKFERLKATQVSKGIRDNERSGRAQVHVSEEGSEPEAMLQVLGPKPDLPQGTEDTAKEDAANRRLAKLYKVSNSGGSMSVSLVADENPFAQSALRSEDCFILDHGRDGKIFVWKGKQANMDERKAALKTASDFISKMQYPRQTQVSVLPEGGETPLFKQFFKNWRDPDQTDGPGLSYLSSHIANVERVPFDAATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVLVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGGTSRDGGQTTPASTRLFQVRASSSGATRAVEVMPKAGALNSNDAFVLKTPSAAYLWVGTGASDAEKTGALELLKVLRAQHVQVEEGSEPDGFWEALGGKTAYRTSPRLKDKKMDAHPPRLFACSNRIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVRQGFEPPSFVGWFLGWDDDYWSVDPLDRALAELAA q6mg76_rat Apom Bat4 C2 C4a Cfb Csnk2b G7e Hspa1a Hspa1b Ly6g5c Ly6g6c Neu1 Ng35 Rps25-ps2 Skiv2l Hspa1l Stk19 protein protein rat Rattus norvegicus (Rat) Rat MMETERLVLPPPDPLNLPLRALEVGCTGRWELLNVPGPPESTLPHGLPPCAPDLCQEAEQLFLSSPAWLPLHGVEHSARKWQRKTDPWSLLAALGTPVPSDLQAQRHPTTGHILGYKEVLLENTNLSATTSLSLRRPPGPASQSLWGNPTQYPFWPGGMDEPSITDLSTREEAEEEIDFEKDLLTVPPGFKKGVDFAPKAPVPGLLSLSRLLEPLDLSGGDEGEGEAAGGPRGDAASASPSSTPLIRASSLEDLVLKEASTVVSTLEPLKPPPQEQWAVPVDVTSPVGDFYRLIPQPAFQWAFEPDVFQKQAILHLEQHDSVFVAAHTSAGKTVVAEYAIALAQKHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPEASCLIMTTEILRSMLYSGSDVIRDLEWVIFDEVHYINDAERGVVWEEVLIMLPEHVSIILLSATVPNALEFADWIGRLKRRQIYVISTVARPVPLEHYLFTGNSPKTQGELFLLLDSRGAFHTKGYYAAVEAKKERMSKHAQTFGAKQPTHQGGPAQDRGVYLALLASLRTRAQLPVVVFTFSRGRCDEQASGLTSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHMSELLRRGLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMRKHDGSTFRDLLPGEYVQMAGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMMGKPSQLQSQFRLTYTMILNLLRVDALRVEDMMKRSFSEFPSRKDSKAHEQALADLTKRLGALEEPDVTGQLADLPEYYSWAEELTETRNMIQRRIMESVNGLKSLSVGRVVVVKNEEHHNALGVILQVSSNSTSRVFTTLVLCDKPAVSENPRDKGPATPDVPHPDDLVGFKLFLPEGPCEHTVAKLQPGDVAAISTKVLRVNGEKISEDFSKRQQPKFRKDPPLAAVTTAVQELLRLAQAYPAGPPTLDPINDLQLKDVAVVEGGLRARKLEELIRGAQCVHSPRFPAQYVKLQERMQIQKEMERLRFLLSDQSLLLLPEYHQRVEVLRTLGYVDEAGTVKLAGRVACAMSSHELLLTELMFDNALSALRPEEIAALLSGLVCQSPGDPGDQLPSTLKQGVERVKAVAKRIGEVQVACGLNQTVEEFVGELNFGLVEVVYEWARGMPFSELAGLSGTPEGLVVRCIQRLAEMCRSLRGAARLVGEPVLGAKMETAATLLRRDIVFAASLYTQ runx2_mouse Runt-related transcription factor 2 Runx2 Aml3 Cbfa1 Osf2 Pebp2a Core-binding factor subunit alpha-1 Acute myeloid leukemia 3 protein Oncogene AML-3 Polyomavirus enhancer-binding protein 2 alpha A subunit SL3-3 enhancer factor 1 alpha A subunit SL3/AKV core-binding factor alpha A subunit Osteoblast-specific transcription factor 2 protein protein mouse Mus musculus Mouse MLHSPHKQPQNHKCGANFLQEDCKKALAFKWLISAGHYQPPRPTESVSALTTVHAGIFKAASSIYNRGHKFYLEKKGGTMASNSLFSAVTPCQQSFFWDPSTSRRFSPPSSSLQPGKMSDVSPVVAAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQEAAAAAAAAAAAAAAAAAAVPRLRPPHDNRTMVEIIADHPAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPREPRRHRQKLDDSKPSLFSDRLSDLGRIPHPSMRVGVPPQNPRPSLNSAPSPFNPQGQSQITDPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLPAITDVPRRISDDDTATSDFCLWPSSLSKKSQAGASELGPFSDPRQFPSISSLTESRFSNPRMHYPATFTYTPPVTSGMSLGMSATTHYHTYLPPPYPGSSQSQSGPFQTSSTPYLYYGTSSASYQFPMVPGGDRSPSRMVPPCTTTSNGSTLLNPNLPNQNDGVDADGSHSSSPTVLNSSGRMDESVWRPY q6ayi1_rat DEAD box protein 5 Ddx5 Probable ATP-dependent RNA helicase DDX5 rCG_35034 protein protein rat Rattus norvegicus (Rat) Rat MSSYSSDRDRGRDRGFGAPRFGGSRTGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVDTYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVANMHNGMNQQAYAYPATAAAAPMIGYPMPTGYSQ q568z8_rat Ddx17 protein protein rat Rattus norvegicus (Rat) Rat MSLPSLSLPHQPAQCLLIDVEDVEDVKFVINYDYPNSSEDYVHRIGRTARSTNKGTAYTFFTPGNLKQARELIKVLEEANQAINPKLMQLVDHRGGGGGGGGRSRYRTTSSANNPNLMYQDECDRRLRGVKDGGRRDSTSYRDRSETDRASYANGSGYGSPNSAFGAQAGQYTYAQGTYGAAAYGTSGYTAQEYAAGTYGASSTTSTGRSSQSSSQQFSGIGRSGQQPQPLMSQQFAQPPGATNMIGYMGQTAYQYPPPPPPPPPSRK runx2_rat Runt-related transcription factor 2 Runx2 Cbfa1 Osf2 Core-binding factor subunit alpha-1 Osteoblast-specific transcription factor 2 protein protein rat Rattus norvegicus (Rat) Rat MLHSPHKQPQNHKCGANFLQEDSKEALVFKWLISAGHYQPPRPTESVSALSTVHAVIFKAASSIYNRGHKFYLEKKGGTMASNSLFSAVTPCQQSFFWNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPREPRRHRQKLDDSKPSLFSDRLSDLGRI ddx5-runx2-2 1290572 n/a 1290577 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1290578 unspecified role unspecified role prey prey c-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false gal4 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro 1290591 1290588 physical association physical association false false false 3E ddx5-runx2-3 1290572 n/a 1290578 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1290577 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 3F ddx17-ddx5 1290573 n/a 1290579 unspecified role unspecified role prey prey n/a 1290580 unspecified role unspecified role prey prey n/a 1290581 unspecified role unspecified role prey prey n/a 1290582 unspecified role unspecified role bait bait n-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1290583 unspecified role unspecified role prey prey n/a 1290584 unspecified role unspecified role prey prey association association false false false 2B Also co-purified but could not be mapped to proteins: alpha-actinin and HSP70 ddx17-ddx5-1 1290574 n/a 1290583 unspecified role unspecified role prey prey n/a 1290582 unspecified role unspecified role bait bait n-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 3A and B ddx5-runx2 1290575 n/a 1290582 unspecified role unspecified role neutral component neutral component n/a 1290578 unspecified role unspecified role neutral component neutral component gal4 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 3D ddx5-runx2-1 1290576 n/a 1290585 unspecified role unspecified role neutral component neutral component n/a 1290583 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3C