UniProt UniProt http://www.uniprot.org psifogeorgou-2007-1 RGS9-2 is a negative modulator of mu-opioid receptor function. rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells confocal microscopy confocal microscopy immunostaining immunostaining Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr J. Neurochem. (0022-3042) 2007 Only protein-protein interactions imex curation psifogeorgou-2007-2 RGS9-2 is a negative modulator of mu-opioid receptor function. rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr J. Neurochem. (0022-3042) 2007 Only protein-protein interactions imex curation psifogeorgou-2007-3 RGS9-2 is a negative modulator of mu-opioid receptor function. rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr J. Neurochem. (0022-3042) 2007 Only protein-protein interactions imex curation psifogeorgou-2007-4 RGS9-2 is a negative modulator of mu-opioid receptor function. rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Psifogeorgou K., Papakosta P., Russo S.J., Neve R.L., Kardassis D., Gold S.J., Zachariou V. HA-MOR plasmid provided by von Zastrow who worked with mouse ( eg. see pmid 12764121 citing pmid 7797593) . Origin of RGS9_2 according pmid 10066255. Co-transfected Gbeta5 could not be mapped (not used as bait or prey protein, respectively). vzachar@med.upc.gr J. Neurochem. (0022-3042) 2007 Only protein-protein interactions imex curation o54828-1 Regulator of G-protein signaling 9 RGS9-2 Rgs9 protein protein mouse Mus musculus Mouse MTIRHQGQQYRPRMAFLQKIEALVKDMQNPETGVRMHNQRVLVTSVPHAMTGGDVLQWITQRLWISNLEAQNLGNFIVKYGYIYPLQDPKNLILKPDSSLYRFQTPYFWPTQQWPAEDTDYAIYLAKRNIKKKGILEEYEKENYDFLNKKINYKWDFVIMQAKEQYRTGKERNKADRYALDCQEKAYWLVHRSPPGMNNVLDYGLDRVTNPNEVKKQTVTAVRKEIMYYQQALMRSTVKSSVSLGGIVKYSEQFSSNDAIMSGCLPSNPWITDDTQFWDLNAKLVEIPTKMRVERWAFNFSELIRDPKGRQSFQYFLKKEFSGENLGFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGKTMDITVKGLRHPHRYVLDAAQTHIYMLMKKDSYARYLKSPIYKEMLAKAIEPQETTKRSSTLPFMRRHLRSSPSPVILRQLEEEEKAREAANTVDITQPGQHLAPSPHLAVYTGTCVPPSPSSPFSPSCRSPRKPFASPSRFIRRPSIAICPSPSRVALEGSSGLEPKGEASWSGANSGPSVTENREPSADHSRPQPRAPPKARAALSLGRFLRRGCLASPVFARLSPKCPSVSHGKVQPLGDMGQQLPRLKPKKVANFFQIKMEMPTDSGTCLMDSDDPRAGESGDQTTEKEVICPWESLAEGKAG oprm_mouse Mu-type opioid receptor Oprm1 Mor Oprm protein protein mouse Mus musculus Mouse MDSSAGPGNISDCSDPLAPASCSPAPGSWLNLSHVDGNQSDPCGPNRTGLGGSHSLCPQTGSPSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGNILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTIEQQNSARIRQNTREHPSTANTVDRTNHQLENLEAETAPLP arrb2_rat Beta-arrestin-2 Arrb2 Arrestin beta-2 protein protein rat Rattus norvegicus (Rat) Rat MGEKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLKDRKVFVTLTCAFRYGREDLDVLGLSFRKDLFIATYQAFPPMPNPPRPPTRLQDRLLKKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKACGVDFEIRAFCAKSIEEKSHKRNSVRLIIRKVQFAPETPGPQPSAETTRHFLMSDRRSLHLEASLDKELYYHGEPLNVNVHVTNNSAKTVKKIRVSVRQYADICLFSTAQYKCPVAQLEQDDQVSPSSTFCKVYTITPLLSDNREKRGLALDGQLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHITLPRPQSAPREIDIPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDCDDQFC oprm1-rgs9 1288232 n/a 1288236 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1288237 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 3 Morphine treatment rgs9-arrb2 1288233 n/a 1288236 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n/a 1288238 unspecified role unspecified role prey prey physical association physical association false false false 4b Morphine treatment Gbeta5 and HA-MOR co-transfected rgs9-oprm1 1288234 n/a 1288236 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n/a 1288237 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4a Morphine treatment Gbeta5 co-transfected oprm1-arrb2 1288235 n/a 1288238 unspecified role unspecified role prey prey n/a 1288237 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4c Overexpression (co-transfection) of RGS9-2 does not affect the interaction after morphine treatment. Morphine treatment.