mpidb The Microbial Protein Interaction Database kajiyama-2007-1 Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis. baciu Bacillus subtilis pull down pull down western blot western blot Immuno blot Immuno blot Kajiyama Y., Otagiri M., Sekiguchi J., Kosono S., Kudo T. J. Bacteriol. (0021-9193) 2007 Only protein-protein interactions imex curation kajiyama-2007-2 Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis. baciu Bacillus subtilis bn-page blue native page bn-page anti tag western anti tag western blot anti tag western Kajiyama Y., Otagiri M., Sekiguchi J., Kosono S., Kudo T. First-dimension BN-PAGE gel and then the second-dimension Tricine SDS-PAGE gel to separate complex members. J. Bacteriol. (0021-9193) 2007 Only protein-protein interactions imex curation mrpa_bacsu Na(+)/H(+) antiporter subunit A Mrp complex subunit A Multiple resistance and pH homeostasis protein A ntrA shaA yufT BSU31600 mrpA protein protein bacsu Bacillus subtilis MQLLHLAILSPFLFAFIIPFLAKYAKRVHTGWFVLILPVLLFIYFLPMIRMTQSGETLRSVLEWIPSLGINFTVYIDGLGLLFALLITGIGSLVTLYSIFYLSKEKEQLGPFYVYLLMFMGAMLGVVLVDNVMVLYMFWELTSLSSFLLIGYWYKREKSRYGAAKSLLITVSGGLCMLGGFILLYLITDSFSIREMVHQVQLIAGHELFIPAMILILLGAFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGIYVIARFSPIFAFSAQWFWIVSLVGLFTMVWGSFHAVKQTDLKSILAFSTVSQLGMIISMLGVSAAALHYGHTEYYTVAAMAAIFHLINHATFKGSLFMAVGIIDHETGTRDIRKLGGLMAIMPITFTISLIGTFSMAGLPPFNGFLSKEMFFTSMLRVTHFDLFNVQTWGVLFPLFAWIGSVFTFIYSMKLLFKTFRGNYQPEQLEKQAHEAPVGMLVPPVILVALAVSLFFFPNILSYSLIEPAMNSIYPTLLDGHEKFHVHISQWHGVTTELLMTAGIVVIGTIGYLSLNKWKGIYKLFPSKLTLNRLYDKLLTMMEKGSYRVTKQYMTGFLRDYLLYIFAGFIILIGGAFAIKGGFSFKTEGMAKIGVYEIILTLVMISATVATVFARSRLTAIIALGVVGYTLALFFVIFRAPDLALTQLVIETISVALFLLCFYHLPKLRLKTKTRTFRMTNFIISLGVGVIVTLLGIASSSQRTKDSIASFFVKHSHDLGGGDNVVNVILVDFRGFDTMFEITVLTIAALGIYSMIKTKVKEEGKSGE mrpe_bacsu Na(+)/H(+) antiporter subunit E Mrp complex subunit E Multiple resistance and pH homeostasis protein E BSU31640 mrpE protein protein bacsu Bacillus subtilis MAFQILLNVFLAFCWMFLSNSPSAAGFITGYILGMLSLFFFRRFFTRQFYLWKLISIIKLCFIFIKELYLANVSVMKSVLSPKLNIRPGIFAFKTELTKDWEITMLSLLITLTPGTLVMDISDDRTILYIHAMDIEDAEKAIFDIRESFEKAIQEVSR mrpb_bacsu Na(+)/H(+) antiporter subunit B Mrp complex subunit B Multiple resistance and pH homeostasis protein B yufU BSU31610 mrpB protein protein bacsu Bacillus subtilis MNEQKTNDLILQTATKLVSFIILLFSFYLFLSGHNAPGGGFVGGLITSSSIVLLLLAYDLKTVRSLLPVNFIYVAGAGLLLAVLTGVGSFVFGAPFLTHTFGYFQLPILGKTELATATIFDLGVYLVVVGITMTIIQTIGEEE mrpc_bacsu Na(+)/H(+) antiporter subunit C Mrp complex subunit C Multiple resistance and pH homeostasis protein C yufV BSU31620 mrpC protein protein bacsu Bacillus subtilis MEILMAVLAGIIFMAATYLLLSKSLLRVIIGTALLSHGVHLMLLTMGGLKKGAAPILSEHAKSFVDPLPQALILTAIVISFGVTSFILVMAFRAYQELKSDDMDQMRGNDQHE mrpd_bacsu Na(+)/H(+) antiporter subunit D Mrp complex subunit D Multiple resistance and pH homeostasis protein D yufD BSU31630 mrpD protein protein bacsu Bacillus subtilis MNNFVILPILIPLLSAILLIFMTKNLMLMRIFSTAASAIGIVISGILVQTVFTKGIQTLSLGGWKAPYGIVLAADQFASLLVLTTAIIGLLVGLYSFRSVGEKRERSFYYSGVQFLLAGVSGAFLTGDLFNMYVFFELLLIASYMLIVLGGTKIQLRESLKYIVFNIVSSALFVIGVGFLYAVTGTLNMADLSVKISESGQTGLITVIGVLLLLVFGMKGGIFPLYFWLPGSYYAPPAAISALFGALLTKVGLYAITRVFTLIFIHDTAFTHQLMIWLAALTVIFGVIGSLAYSNVMKIVIYNIITAVGVILFGVAVHTPASIQGAIYYLIHDMLIKGALFMLAGTLIALTGTASLHKMGGLIKRYPVLGWMFFISAISLAGIPPLSGFVGKFKIAEGGFAEGEFTISMLILLSSLLVLYSVLRIFIHAFWGEEKETPKPNHRTAKGLLYPAAIFLLLSLLFGLGTEWVSPYVDQAAETLLNPEKYIEAVLKE mrpf_bacsu Na(+)/H(+) antiporter subunit F Mrp complex subunit F Multiple resistance and pH homeostasis protein F Sodium-cholate efflux protein MrpF yufC BSU31650 mrpF protein protein bacsu Bacillus subtilis MFTLILQIALGIMAVSTFLYVIRVIKGPTVPDRVVALDAIGINLIAITALVSILLKTSAFLDIILLLGILSFIGTIAFSKFLEKGEIIENDRNR mrpg_bacsu Na(+)/H(+) antiporter subunit G Mrp complex subunit G Multiple resistance and pH homeostasis protein G yufB BSU31660 mrpG protein protein bacsu Bacillus subtilis MIETAKVVVAVFILLGALICLIASFGVLRLPDVFTRAHAASKGSTLGVNMILLGVFFYLWFVTGELSAKILLGILFIFITSPIGGHLICRAAYNSGVKLDERSVQDDYNGIRNFVIKRKEDSYL mrpa-mrpe 1332063 not-defined-661615882 1332065 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false not-defined-952323465 1332066 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2 mrpb-mrpe 1332063 not-defined-1636361479 1332066 unspecified role unspecified role prey prey not-defined-991368557 1332067 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 2 mrpc-mrpe 1332063 not-defined-2095752684 1332066 unspecified role unspecified role prey prey not-defined-1229906250 1332068 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 2 mrpd-mrpe 1332063 not-defined-873227722 1332066 unspecified role unspecified role prey prey not-defined-2024939356 1332069 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 2 mrpe-mrpe 1332063 not-defined-1709265646 1332066 unspecified role unspecified role bait bait not-defined-2014079244 1332066 unspecified role unspecified role prey prey physical association physical association false false false mrpf-mrpe 1332063 not-defined-862981110 1332066 unspecified role unspecified role prey prey not-defined-1689704684 1332070 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 2 mrpg-mrpe 1332063 not-defined-1738673184 1332071 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false not-defined-2021528211 1332066 unspecified role unspecified role prey prey physical association physical association false false false mrpa-mrpb 1332064 not-defined-1934892269 1332066 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false not-defined-1363166915 1332065 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1332070 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1332067 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1332069 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1332071 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1332068 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 3