mpidb The Microbial Protein Interaction Database chang-2007-1 The N-terminal propeptide of Vibrio vulnificus extracellular metalloprotease is both an inhibitor of and a substrate for the enzyme. in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Chang A.K., Park J.W., Lee E.H., Lee J.S. J. Bacteriol. (0021-9193) 2007 Only protein-protein interactions imex curation c3vhn8_vibvl Neutral metalloproteinase vvpE protein protein vibvl Vibrio vulnificus MKHNQRHRLGLMIAAVVCSLPVTAAEMVSVSDSAVLEQALSVQARSLAPVENGFEAVKTIQLPNGKTKVRYQQTYLGLPVFDTAVVATEGRSGLSNVHGTMAQGLAADLPSVSVNLDQQHAIALGKQRHQAKHANAKALNTENETAQLMVRLDANNKAQLVYLVSFFVAEEEPSRPFMFIDANSGEILQVWDGLNHAQADGTGPGGNSKTGRYEFGTDYPSFVIDKVGTTCTMENSVVKTVDLQNRTSGSTAYSYSCPGASNYNDHKAVNGAYSPLNDAHYFGKVVYDMYKDWMNTAPLTFKLTMRVHYSSNYENAFWDGSAMTFGDGASTFYPLVDINVSAHEVSHGFTEQNSGLIYSNMSGGMNEAFSDIAGEAAEFYMKGSVDWIVGADIFKSNGGLRYFDQPSKDGRSIDHASQYYDGLNVHHSSGVYNRAFYLLANKTGWNVRKGFEIFTLANQLYWTANSTFDAGACGVVKAAQDMGYNSNDVAEAFNQVGVNANCGVTPPSGNVLKNNTPVSNLTGNKGSEVFYTFTVDRNATAVVSISGGSGDADLYLKAGSKPTTSSWDCRPYRYGNNESCSVSAAPGTTYHVMIKGYSNYSGVTLKLQY vvpe-vvpe-2 1332016 not-defined-1959582155 1332017 enzyme enzyme unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-1444981286 1332017 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1332023 1332020 physical association physical association false false false Fig. 6, 7 The N-terminal propeptide inhibited the activities of both the mature protease plus the C-terminal propeptide, and only the mature protease but this could not be fully captured as the substrate used for assessing protease activity was a mixture of proteins.