UniProt UniProt http://www.uniprot.org lopez_garcia-2006-5 ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation lopez_garcia-2006-2 ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation lopez_garcia-2006-3 ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation lopez_garcia-2006-1 ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation lopez_garcia-2006-4 ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation lopez_garcia-2006-6 ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Origin of ERalpha according sequence length. Origin of CTBP1 according ref 40 (pmid 8440238). Origin of RIP140 according sequence length. Exp. with radiolabeled HDAC proteins (fig. 6C) omitted as species origin cannot be established. simak.ali@imperial.ac.uk human placental cDNA expression library (BD Biosciences, UK). Nucleic Acids Res. (0305-1048) 2006 mimix curation zn366_human Zinc finger protein 366 ZNF366 Dendritic cell-specific transcript protein protein protein human Homo sapiens Human MQKEMKMIKDEDVHFDLAVKKTPSFPHCLQPVASRGKAPQRHPFPEALRGPFSQFRYEPPPGDLDGFPGVFEGAGSRKRKSMPTKMPYNHPAEEVTLALHSEENKNHGLPNLPLLFPQPPRPKYDSQMIDLCNVGFQFYRSLEHFGGKPVKQEPIKPSAVWPQPTPTPFLPTPYPYYPKVHPGLMFPFFVPSSSPFPFSRHTFLPKQPPEPLLPRKAEPQESEETKQKVERVDVNVQIDDSYYVDVGGSQKRWQCPTCEKSYTSKYNLVTHILGHSGIKPHACTHCGKLFKQLSHLHTHMLTHQGTRPHKCQVCHKAFTQTSHLKRHMMQHSEVKPHNCRVCGRGFAYPSELKAHEAKHASGRENICVECGLDFPTLAQLKRHLTTHRGPIQYNCSECDKTFQYPSQLQNHMMKHKDIRPYICSECGMEFVQPHHLKQHSLTHKGVKEHKCGICGREFTLLANMKRHVLIHTNIRAYQCHLCYKSFVQKQTLKAHMIVHSDVKPFKCKLCGKEFNRMHNLMGHMHLHSDSKPFKCLYCPSKFTLKGNLTRHMKVKHGVMERGLHSQGLGRGRIALAQTAGVLRSLEQEEPFDLSQKRRAKVPVFQSDGESAQGSHCHEEEEEDNCYEVEPYSPGLAPQSQQLCTPEDLSTKSEHAPEVLEEACKEEKEDASKGEWEKRSKGDLGAEGGQERDCAGRDECLSLRAFQSTRRGPSFSDYLYFKHRDESLKELLERKMEKQAVLLGI esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV nrip1_human Nuclear receptor-interacting protein 1 Nuclear factor RIP140 Receptor-interacting protein 140 NRIP1 protein protein human Homo sapiens Human MTHGEELGSDVHQDSIVLTYLEGLLMHQAAGGSGTAVDKKSAGHNEEDQNFNISGSAFPTCQSNGPVLNTHTYQGSGMLHLKKARLLQSSEDWNAAKRKRLSDSIMNLNVKKEALLAGMVDSVPKGKQDSTLLASLLQSFSSRLQTVALSQQIRQSLKEQGYALSHDSLKVEKDLRCYGVASSHLKTLLKKSKVKDQKPDTNLPDVTKNLIRDRFAESPHHVGQSGTKVMSEPLSCAARLQAVASMVEKRASPATSPKPSVACSQLALLLSSEAHLQQYSREHALKTQNANQAASERLAAMARLQENGQKDVGSYQLPKGMSSHLNGQARTSSSKLMASKSSATVFQNPMGIIPSSPKNAGYKNSLERNNIKQAANNSLLLHLLKSQTIPKPMNGHSHSERGSIFEESSTPTTIDEYSDNNPSFTDDSSGDESSYSNCVPIDLSCKHRTEKSESDQPVSLDNFTQSLLNTWDPKVPDVDIKEDQDTSKNSKLNSHQKVTLLQLLLGHKNEENVEKNTSPQGVHNDVSKFNTQNYARTSVIESPSTNRTTPVSTPPLLTSSKAGSPINLSQHSLVIKWNSPPYVCSTQSEKLTNTASNHSMDLTKSKDPPGEKPAQNEGAQNSATFSASKLLQNLAQCGMQSSMSVEEQRPSKQLLTGNTDKPIGMIDRLNSPLLSNKTNAVEENKAFSSQPTGPEPGLSGSEIENLLERRTVLQLLLGNPNKGKSEKKEKTPLRDESTQEHSERALSEQILMVKIKSEPCDDLQIPNTNVHLSHDAKSAPFLGMAPAVQRSAPALPVSEDFKSEPVSPQDFSFSKNGLLSRLLRQNQDSYLADDSDRSHRNNEMALLESKNLCMVPKKRKLYTEPLENPFKKMKNNIVDAANNHSAPEVLYGSLLNQEELKFSRNDLEFKYPAGHGSASESEHRSWARESKSFNVLKQLLLSENCVRDLSPHRSNSVADSKKKGHKNNVTNSKPEFSISSLNGLMYSSTQPSSCMDNRTFSYPGVVKTPVSPTFPEHLGCAGSRPESGLLNGCSMPSEKGPIKWVITDAEKNEYEKDSPRLTKTNPILYYMLQKGGNSVTSRETQDKDIWREASSAESVSQVTAKEELLPTAETKASFFNLRSPYNSHMGNNASRPHSANGEVYGLLGSVLTIKKESE ctbp1_human C-terminal-binding protein 1 CTBP1 CTBP protein protein human Homo sapiens Human MGSSHLLNKGLPLGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHASDQL znf366-esr1-1 1266803 n/a 1266809 unspecified role unspecified role bait bait n/a 1266810 unspecified role unspecified role prey prey direct interaction direct interaction false false false 3C and D nrip1-znf366 1266803 n/a 1266811 unspecified role unspecified role bait bait n/a 1266809 unspecified role unspecified role prey prey direct interaction direct interaction false false false 5A nrip1-ctbp1 1266803 n/a 1266812 unspecified role unspecified role prey prey n/a 1266811 unspecified role unspecified role bait bait direct interaction direct interaction false false false 5A esr1-znf366-1 1266803 n/a 1266810 unspecified role unspecified role bait bait n/a 1266809 unspecified role unspecified role prey prey direct interaction direct interaction false false false 5A znf366-nrip1 1266803 n/a 1266809 unspecified role unspecified role bait bait n/a 1266811 unspecified role unspecified role prey prey direct interaction direct interaction false false false 5B znf366-ctbp1 1266803 n/a 1266809 unspecified role unspecified role bait bait n/a 1266812 unspecified role unspecified role prey prey direct interaction direct interaction false false false 5B ctbp1-znf366 1266803 n/a 1266809 unspecified role unspecified role prey prey n/a 1266812 unspecified role unspecified role bait bait direct interaction direct interaction false false false 5C znf366-esr1 1266804 n/a 1266810 unspecified role unspecified role prey prey n/a 1266809 unspecified role unspecified role bait bait physical association physical association false false false 2A znf366-ctbp1-2 1266804 n/a 1266809 unspecified role unspecified role bait bait n/a 1266812 unspecified role unspecified role prey prey physical association physical association false false false 5G esr1-znf366-1 1266805 n/a 1266810 unspecified role unspecified role bait bait n/a 1266809 unspecified role unspecified role prey prey physical association physical association false false false 2B ctbp1-znf366-1 1266805 n/a 1266812 unspecified role unspecified role bait bait n/a 1266809 unspecified role unspecified role prey prey physical association physical association false false false 5F esr1-znf366 1266806 n/a 1266809 unspecified role unspecified role prey prey n/a 1266810 unspecified role unspecified role bait bait physical association physical association false false false no figure esr1-znf366-2 1266807 n/a 1266809 unspecified role unspecified role prey prey n/a 1266810 unspecified role unspecified role bait bait physical association physical association false false false 2C znf366-ctbp1-1 1266808 n/a 1266809 unspecified role unspecified role bait bait n/a 1266812 unspecified role unspecified role prey prey physical association physical association false false false 5D