mbinfo MBInfo http://www.mechanobio.info/ http://www.mechanobio.info/ crawford-1992-2 An interaction between zyxin and alpha-actinin. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting autoradiography autoradiography Crawford A.W., Michelsen J.W., Beckerle M.C. The shift in the elution profile of zyxin when preincubated with alpha-actinin and that a portion of zyxin co-elutes with alpha-actinin suggest that there is an association of zyxin with alpha-actinin. Infered from the paper, both alpha-actinin and zyxin proteins were purified from the smooth muscle of chick gizzard. Alpha-actinin-1 isoform 2 corresponds to the type of alpha-actinin-1 in the smooth muscle. The contact email was not given by the author. J. Cell Biol. (0021-9525) 1992 Only protein-protein interactions imex curation crawford-1992-1 An interaction between zyxin and alpha-actinin. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Crawford A.W., Michelsen J.W., Beckerle M.C. The shift in the elution profile of zyxin when preincubated with alpha-actinin and that a portion of zyxin co-elutes with alpha-actinin suggest that there is an association of zyxin with alpha-actinin. Infered from the paper, both alpha-actinin and zyxin proteins were purified from the smooth muscle of chick gizzard. Alpha-actinin-1 isoform 2 corresponds to the type of alpha-actinin-1 in the smooth muscle. The contact email was not given by the author. J. Cell Biol. (0021-9525) 1992 Only protein-protein interactions imex curation crawford-1992-3 An interaction between zyxin and alpha-actinin. in vitro In vitro solid phase assay solid phase assay autoradiography autoradiography Crawford A.W., Michelsen J.W., Beckerle M.C. The shift in the elution profile of zyxin when preincubated with alpha-actinin and that a portion of zyxin co-elutes with alpha-actinin suggest that there is an association of zyxin with alpha-actinin. Infered from the paper, both alpha-actinin and zyxin proteins were purified from the smooth muscle of chick gizzard. Alpha-actinin-1 isoform 2 corresponds to the type of alpha-actinin-1 in the smooth muscle. The contact email was not given by the author. J. Cell Biol. (0021-9525) 1992 Only protein-protein interactions imex curation zyx_chick Zyxin ZYX protein protein chick Gallus gallus (Chicken) Chicken MASPGTPGTRMTTTVSINISTPSFYNPQKKFAPVVAPKPKVNPFKTGGTSESSQPQPPGTGAQRAQIGRVGEIPVSVTAEELPLPPPPPPGEELSFSSNCAFPPPPPPFEEPFPPAPDEAFPSPPPPPPPMFDEGPALQIPPGSTGSVEKPLAPKAHVEISSAPRDPTPPFPSKFTPKPSGTLSSKPPGLDSTPAPAPWAAPQQRKEPLASVPPPPSLPSQPTAKFTPPPVASSPGSKPGATVPMAPSNSTRYPTSLQTQFTAPSPSGPLSRPQPPNFTYAQQWERPQVQEKPVPTEKSAAVKDMRRPTADPPKGNSPLTMKEVEELELLTQKLMKDMDHPPPVEAATSELCGFCRKPLSRTQPAVRALDCLFHVECFTCFKCEKQLQGQQFYNVDEKPFCEDCYAGTLEKCSVCKQTITDRMLKATGNSYHPQCFTCVMCHTPLEGASFIVDQANQPHCVDDYHRKYAPRCSVCSEPIMPEPGKDETVRVVALEKNFHMKCYKCEDCGRPLSIEADENGCFPLDGHVLCMKCHTVRAKTAC p05094-2 Alpha-actinin-1 Smooth muscle ACTN1 Alpha-actinin cytoskeletal isoform F-actin cross-linking protein Non-muscle alpha-actinin-1 protein protein chick Gallus gallus (Chicken) Chicken MDHHYDPQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNAWGGLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHESWTDGKEAMLQQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERQAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWEHVRQLVPRRDQALMEEHARQQQNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQQIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDCEDFRACLISMGYNMGEAEFARIMSIVDPNRMGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYNGRDAVPGALDYMSFSTALYGESDL zyx-actn1 1306975 n/a 1306978 unspecified role unspecified role bait bait region 125i radiolabel 125i radiolabel I125 125I 125I I125 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chick-gizzard Chicken gizzard gizzard gizzard n/a actn1 1306979 unspecified role unspecified role prey prey chick-gizzard Chicken gizzard gizzard gizzard physical association physical association false false false Fig. 3B& 4B Purified alpha-actinin was used in Fig 3B, while a mixture of proteins from chick gizzard extracts was used in Fig 4B. Zyxin binds to the 27kDa fragment of alpha-actinin-1, but not the 53 kDa fragment.(Fig.3B) actn1-zyx 1306975 n/a actn1 1306979 unspecified role unspecified role bait bait region 125i radiolabel 125i radiolabel I125 125I 125I I125 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chick-gizzard Chicken gizzard gizzard gizzard n/a 1306978 unspecified role unspecified role prey prey chick-gizzard Chicken gizzard gizzard gizzard physical association physical association false false false Fig. 5B The heat denatured alpha-actinin does not bind zyxin. actn1-zyx 1306976 n/a actn1 1306979 unspecified role unspecified role neutral component neutral component chick-gizzard Chicken gizzard gizzard gizzard n/a 1306978 unspecified role unspecified role neutral component neutral component chick-gizzard Chicken gizzard gizzard gizzard physical association physical association false false false Fig. 2B zyx-actn1 1306977 n/a 1306978 unspecified role unspecified role bait bait chick-gizzard Chicken gizzard gizzard gizzard n/a actn1 1306979 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chick-gizzard Chicken gizzard gizzard gizzard physical association physical association false false false 1306977 Fig. 6 & 7A,B The association between zyxin and 125 i-alpha-actinin was competitively inhibited by unlabeled alpha-actinin (Fig. 6&7a).