UniProt UniProt http://www.uniprot.org pan-2004a-1 Localization of two homologous Arabidopsis kinesin-related proteins in the phragmoplast. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Pan R., Lee Y.R., Liu B. bliu@ucdavis.edu Planta (0032-0935) 2004 Only protein-protein interactions imex curation kn12a_arath Kinesin-like protein KIN-12A KIN12A PAKRP1 Phragmoplast-associated kinesin-related protein 1 FCAALL.159 dl3115c At4g14150 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKKHFTLPRNAILRDGGEPHSPNPSISKSKPPRKLRSAKENAPPLDRNTSTPDHRSMRMKNPLPPRPPPSNPLKRKLSAETATESGFSDSGVKVIVRMKPLNKGEEGDMIVEKMSKDSLTVSGQTFTFDSIANPESTQEQMFQLVGAPLVENCLSGFNSSVFAYGQTGSGKTYTMWGPANGLLEEHLCGDQRGLTPRVFERLFARIKEEQVKHAERQLNYQCRCSLLEIYNEQITDLLDPSQKNLMIREDVKSGVYVENLTEEYVKNLTDVSQLLIKGLGNRRTGATSVNTESSRSHCVFTCVVESRCKNVADGLSSFKTSRINLVDLAGSERQKSTGAAGERLKEAGNINRSLSQLGNLINILAEISQTGKPRHIPYRDSRLTFLLQESLGGNAKLAMVCAVSPSQSCRSETFSTLRFAQRAKAIQNKAVVNEVMQDDVNFLRGVIHQLRDELQRMKNDGNNPTNPNVAYSTAWNARRSLNLLRSFGLGHPRSLPHEDNDGDIEMEIDEAAVERLCVQVGLQSSLASEGINHDMNRVKSIHSSDGQSIEKRLPEDSDVAMEDACCHTENHEPETVDNMRTETETGIRENQIKTHSQTLDHESSFQPLSVKDALCSSLNKSEDVSSCPDLVPQDVTSANVLIADGVDDPEHLVNSASPSLCIDPVGATPVLKSPTLSVSPTIRNSRKSLKTSELSTASQKDSEGENLVTEAADPSPATSKKMNNCSSALSTQKSKVFPVRTERLASSLHKGIKLLESYCQSTAQRRSTYRFSFKAPDSEPSTSISKADAGVQTIPGADAISEENTKEFLCCKCKCREQFDAQQMGDMPNLQLVPVDNSEVAEKSKNQVPKAVEKVLAGSIRREMALEEFCTKQASEITQLNRLVQQYKHERECNAIIGQTREDKIIRLESLMDGVLSKEDFLDEEFASLLHEHKLLKDMYQNHPEVLKTKIELERTQEEVENFKNFYGDMGEREVLLEEIQDLKLQLQCYIDPSLKSALKTCTLLKLSYQAPPVNAIPESQDESLEKTLEQERLCWTEAETKWISLSEELRTELEASKALINKQKHELEIEKRCGEELKEAMQMAMEGHARMLEQYADLEEKHMQLLARHRRIQDGIDDVKKAAARAGVRGAESRFINALAAEISALKVEKEKERQYLRDENKSLQTQLRDTAEAIQAAGELLVRLKEAEEGLTVAQKRAMDAEYEAAEAYRQIDKLKKKHENEINTLNQLVPQSHIHNECSTKCDQAVEPSVNASSEQQWRDEFEPLYKKETEFSNLAEPSWFSGYDRCNI kn12b_arath Kinesin-like protein KIN-12B KIN12B PAKRP1L Phragmoplast-associated kinesin-related protein 1-like protein MDB19.16 At3g23670 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKHFMMPRNAILRDIGESQSPNPSLTKSKSQRKIKSSKENAPPPDLNSLIPDHRSSPAKLKSPLPPRPPSSNPLKRKLIAEATADNGVAIGVSDSGVKVIVRMKPPSKGEEEEMIVKKISNDALTINEQTFTFDSIADPESTQDEIFQLVGAPLVENCLAGFNSSVFAYGQTGSGKTYTMWGPANGLLEEHLSGDQRGLTPRVFELLFARLSEEQAKHAERQLKYQCRCSFLEIYNEQITDLLDPSLKNLMIREDVKSGVYVENLTEEYVKNLKDLSKLLVKGLANRRTGATSVNAESSRSHCVFTCVVESHCKSVADGLSSFKTSRINLVDLAGSERQKLTGAAGDRLKEAGNINRSLSQLGNLINILAEISQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMVCAVSPSQSCRSETFSTLRFAQRAKAIQNKAIVNEVMQDDVNFLREVIRQLRDELQRVKDDKGNNPTNPNAAYTTSWNARRSLSLLRSFGLGHPKSLPNGDDDGDTEMEIDEEAVERLCAQMGLSPPAEDNNQEMSRVEKINSSLQTVVLKDESYNNSHLKSSEATDVNMEDACCQTENNGSETDNALTVAETMDDGSSVQPDSITNSLHSCISDTNQGNSPSKAENIPSCQDLVIEADVSAIVSVADTSNNTEQVSVNPVSPCLSVAPVSVSPVLIPPTESASPKIRNSRKSLRTTSMSTASQKDIERANQLTPEVVEPSPAMSTEVLNLYSALSTKKSEAFPVPTRQLAASLHRGMKLLDSYRQSTALRRSTFRLSYKALECKPSTVLSKADVGVQTYPQADEIAEDNSKEVLCSRCKCRAECDAQEISDTSNLQLVPIDNSEGSEKSNFQVPKAVEKVLAGSIRREMAMEEFCTKQASEISQLNRLVQQYKHERECNAIIGQTREDKIVRLESLMDGVLSKDDFLDEEFASLMHEHKLLKDMYENHPEVLQTRIELKRVQEELESFKNFYGDMGEREVLLEEIHDLKAQLQCYTDSSLTSARRRGSLLKLTYACDPNQAPQLNTIPESVDEGPEKTLEQERLRWTEAESNWISLAEELRTELDTNRLLMEKQKRELDTEKRCAEELTEAMQMAMQGHARMIEQYADLEEKHIQLLARHRRIREGIDDVKKAAARAGVKGAESRFINALAAEISALKVQREKEVRYFRDENKSLQSQLRDTAEAVQAAGELLVRFKEAEEGLTFAQKRAMDAEYEASEAYKKVDKLKRKYETEISTVNQQHNAEPQNPIESLQASCNDDAMAKYDEPSASDGDNQWREEFQPFYKKDEELSKLAEPSWFSGYDRCNI kinesin_12b-pakrp1 1315085 n/a 1315086 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1315086 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro 1315094 1315091 physical association physical association false false false Fig. 6 lane 2 kinesin_12b-pakrp1-1 1315085 n/a 1315086 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1315087 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro 1315101 1315098 physical association physical association false false false Fig. 6 lane 3 pakrp1-kinesin_12b 1315085 n/a 1315086 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1315087 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro 1315107 1315105 physical association physical association false false false Fig. 6 lane 6 kinesin_1-kinesin_-1 1315085 n/a 1315087 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 1315087 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro 1315115 1315111 physical association physical association false false false Fig. 6 lane 7