IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ agne-2003-1 Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. yeast Saccharomyces cerevisiae Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation agne-2003-2 Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. yeast Saccharomyces cerevisiae Baker's yeast pull down pull down sequence tag sequence tag identification sequence tag Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation agne-2003-3 Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. yeast Saccharomyces cerevisiae Baker's yeast bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation agne-2003-4 Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. yeast Saccharomyces cerevisiae Baker's yeast bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Agne B., Meindl N.M., Niederhoff K., Einwachter H., Rehling P., Sickmann A., Meyer H.E., Girzalsky W., Kunau W.H. Protein-A bait constructs include a TEV cleavage site, which is utilised to cleave the complexes off the resin. wolf-h.kunau@ruhr-uni-bochum.de Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation pex12_yeast Peroxisome assembly protein 12 PEX12 Peroxin-12 YM9711.16C YMR026C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFYSNLPSAGQSSRGSSTSGRNGVGLEPLYPTIFEIMSSQEIDSLLPASIRYLLANHLVANFPNRYTLRLNKYFFEWFQAIKGFVEWYHLKTYNSTFIDRFYGLQLFSSRDRNLALTQCLNPKGQSEWPQGLQLNQQQKSVIFLEKIILPYITAKLDEILEKISMNNIFSSDETENKWPKRAFLRIYPFIKKLLALSNLLVKLLFLTKRTGSVSLLQYLFKIEYTTVRPLSSELSGLKETKGMDNRLRKTNISSIFALMQGQLSIIPRFLTFMGSQFFPTFIFVLRVYQWWTTQDMTTKLQKRVNDLDEDIPRPPFSSHSDKTEDKEGVSEACPVCEKTVQNPCVLETGYVACYPCAISYLVNNEGHCPVTNKKLLGCTYNKHTNKWEVVTGIRKLLI 98E6300C2CBFA16A pex13_yeast Peroxisomal membrane protein PAS20 PEX13 PAS20 YLR191W L9470.1 Peroxin-13 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSTAVPRPKPWETSASLEEPQRNAQSLSAMMTSNQQDSRPTEESNNSNSASESAPEVLPRPAALNSSGTYGESNTIPGIYGNSNYGIPYDNNPYSMNSIYGNSIGRYGYGGSYYGNNYGSFYGGGYGAGAGYGMNNGSGLGESTKATFQLIESLIGAVTGFAQMLESTYMATHNSFFTMISVAEQFGNLKEMLGSFFGIFAIMKFLKKILYRATKGRLGIPPKNFAESEGSKNKLIEDFQKFNDSGTINSNEKATRRKISWKPLLFFLMAVFGFPYLLNKFITKLQTSGTIRASQGNGSEPIDPSKLEFARALYDFVPENPEMEVALKKGDLMAILSKKDPLGRDSDWWKVRTKNGNIGYIPYNYIEIIKRRKKIEHVDDETRTH DBEA9A2372185860 pex17_yeast Peroxisomal membrane protein PEX17 PEX17 PAS9 YNL214W N1319 Peroxin-17 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSINSFPRNIDWPSNIGIKKIEGTNPTVNAIKGLLYNGGSIYAFLYFVIAMFVEPTLQKQYQQRNDFSLFVLLRLRRIIAQLQKRLVMTPVSSLGFNEQNNFVERSTQTSDDNIIREDNSHWAEMIYQLQNMKQELQYFNRSSGQPSESIDDFVFQIKMVTDQVELTDRSRAFSNKSRNIIQGIREIKGWFVNGQVPR pex15_yeast Peroxisomal membrane protein PEX15 Peroxisome biosynthesis protein PAS21 PEX15 Peroxin-15 PAS21 YOL044W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAASEIMNNLPMHSLDSSLRDLLNDDLFIESDESTKSVNDQRSEVFQECVNLFIKRDIKDCLEKMSEVGFIDITVFKSNPMILDLFVSACDIMPSFTKLGLTLQSEILNIFTLDTPQCIETRKIILGDLSKLLVINKFFRCCIKVIQFNLTDHTEQEEKTLELESIMSDFIFVYITKMRTTIDVVGLQELIEIFIFQVKVKLHHKKPSPNMYWALCKTLPKLSPTLKGLYLSKDVSIEDAILNSIDNKIQKDKAKSKGKQRGVKQKIHHFHEPMLHNSSEEQVKVEDAFNQRTSTDSRLQSTGTAPRKKNNDITVLAGSFWAVLKHHFTRSVLNKNGLLLTGLLLLLCLKKYKSLMAIFKHVPAAFHTVYPQIVGLLKLLASI B575A8D4B0DAC0B8 pex10_yeast Peroxisome biogenesis factor 10 PAS4 YDR265W D9954.8 PEX10 Peroxin-10 Peroxisome assembly protein 10 Peroxisomal biogenesis factor 10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKNDNKLQKEALMRLSQLRFPFADAPSIVQAHQKDEQIQGLLIMKVTELCKLIKSQLFVNSYPKELSIFAKLLYLLFTTGRRGRTLGEEYVDLTYTNRKGTRLAGRLKMIVFAFAYPLCPYFITKLYKKIMKNNKESKIEDTESVAAFCKGLLDFILDVHMTLFYFKGAFYSISKRIFGMRYVFKHILSKNEANFREEGSQKYKVLGYILLAQNVMKWYPVLTSTLGSWIYGRKRTNDSITRSSVGLQERSEHESIEGIPKESQLTHINLSDKNQLPFIPEASRKCILCLMNMSDPSCAPCGHLFCWSCLMSWCKERPECPLCRQHCQPQEILVLRQ pex14_yeast Peroxisomal membrane protein PEX14 PEX14 YGL153W G1870 Peroxin-14 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDVVSKDRKALFDSAVSFLKDESIKDAPLLKKIEFLKSKGLTEKEIEIAMKEPKKDGIVGDEVSKKIGSTENRASQDMYLYEAMPPTLPHRDWKDYFVMATATAGLLYGAYEVTRRYVIPNILPEAKSKLEGDKKEIDDQFSKIDTVLNAIEAEQAEFRKKESETLKELSDTIAELKQALVQTTRSREKIEDEFRIVKLEVVNMQNTIDKFVSDNDGMQELNNIQKEMESLKSLMNNRMESGNAQDNRLFSISPNGIPGIDTIPSASEILAKMGMQEESDKEKENGSDANKDDNAVPAWKKAREQTIDSNASIPEWQKNTAANEISVPDWQNGQVEDSIP B714D7DA045E2DCB pex5_yeast Peroxisomal targeting signal receptor PEX5 PAS10 YDR244W YD8419.11 Peroxisomal protein PAS10 Peroxin-5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDVGSCSVGNNPLAQLHKHTQQNKSLQFNQKNNGRLNESPLQGTNKPGISEAFISNVNAISQENMANMQRFINGEPLIDDKRRMEIGPSSGRLPPFSNVHSLQTSANPTQIKGVNDISHWSQEFQGSNSIQNRNADTGNSEKAWQRGSTTASSRFQYPNTMMNNYAYASMNSLSGSRLQSPAFMNQQQSGRSKEGVNEQEQQPWTDQFEKLEKEVSENLDINDEIEKEENVSEVEQNKPETVEKEEGVYGDQYQSDFQEVWDSIHKDAEEVLPSELVNDDLNLGEDYLKYLGGRVNGNIEYAFQSNNEYFNNPNAYKIGCLLMENGAKLSEAALAFEAAVKEKPDHVDAWLRLGLVQTQNEKELNGISALEECLKLDPKNLEAMKTLAISYINEGYDMSAFTMLDKWAETKYPEIWSRIKQQDDKFQKEKGFTHIDMNAHITKQFLQLANNLSTIDPEIQLCLGLLFYTKDDFDKTIDCFESALRVNPNDELMWNRLGASLANSNRSEEAIQAYHRALQLKPSFVRARYNLAVSSMNIGCFKEAAGYLLSVLSMHEVNTNNKKGDVGSLLNTYNDTVIETLKRVFIAMNRDDLLQEVKPGMDLKRFKGEFSF pex8_yeast Peroxisomal biogenesis factor 8 YGR077C PEX8 PAS6 Peroxin-8 Peroxisomal protein PAS6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFDHDVEYLITALSSETRIQYDQRLLDEIAANVVYYVPRVKSPDTLYRLVGALFRSQFIVQLPPLRLLHIVKDVFLWKLEVSEPTLPISKFYLVWNAVFESHRATWNLSQLMVLDGVLVTYPSFKQLNNAYFIDESSNKTALYYRNWKLQLFSPIWAQLWNTAIVRANLSIQHCLLIALALLFNQSNRSALLHGVDVSWNLVTEKLLDLLEEYVHGIVQPMEIFSTDSVLSTNLNHLASCLTSSITRSNEATLVNSVRKLERICRYLSDTVASLKEQQLDFKFQNVFILIILALKELSAMNMTILPNHKDTFYSMICLSLFHVHVLTQKIGTVGFPSYDYVYDNLVTYFIVMDDLSKITTVLELMKRNNTKQDPNKLVFYINFLNKITNYYGCRIRLPFITEFIEPLLHFDVFFSGKTGNTLDIEIKESIHTLTITVLSIDSSYSSQVAQWQVSRILVYLKMSMDQFIAGKLSANQILLIFGHLSTQLPSLHNYNKHLLRDSLHETYIRIVNVKNPEKKNVLIECLIVQIAFINNPHHLIGWLNICLQLINTHNKKLLQQLWEMVSSLESSLAIDWWYTTVLSSQSSKL pex2_yeast Peroxisomal biogenesis factor 2 PEX2 CRT1 PAS5 YJL210W J0240 HRA271 Peroxin-2 Peroxisomal protein PAS5 Protein CRT1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRVAQLDSIALDKELYGQFWSEFNAAFNTSEHKEEWELALNTVVFMCATRFLPHYGSSCTYGSALSGVVFQCRKRTLYVVTVLAGYVWKKITHIIFNGPHCGNQMMWLKLYKWVNLLYHGCDVTNFLRFLAAEGPNARAFLSPLYRAFNVHSTRLIRDGSAIASEFYSNSVFAGLEYQNRQLLWNALLELFSNTLLTKRGLLTFVKKPPRSRSTTTYKTVCPRCGGFPTNPYQIACCRANYCYVCVVKALEWSMCDACGSSGRLTASPVY FAEFA3A526E82F3E pex14-pex12 1287642 n/a 1287646 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287649 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 1A Membrane extraction with Digitonin pex14-pex15-1 1287642 n/a 1287648 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287652 unspecified role unspecified role prey prey association association false false false Fig. 1A Membrane extraction using n-dodecyl-beta-D-maltopyranosid (DDM.) pex14-pex17-2 1287642 n/a 1287651 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287648 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A Membrane extraction using CYMAL-4 pex14-pex10 1287642 n/a 1287649 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287653 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287650 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey association association false false false Fig. 1C Membrane extraction with 1% Digitonin pex2-pex10 1287642 n/a 1287651 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287653 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287654 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 1C Membrane extraction with 1% Digitonin pex2-pex10-2 1287642 n/a 1287654 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287650 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey association association false false false Fig. 3A Membrane extraction with 1% Digitonin. Observed in wild-type, PEX1-/- and PEX4-/-cells. pex2-pex10-3 1287642 n/a 1287646 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287654 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 3A Membrane extraction with 1% Digitonin. Observed in Pex5-/- cells pex2-pex10-4 1287642 n/a 1287654 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287650 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey association association false false false Fig. 3A Membrane extraction with 1% Digitonin. Observed in Pex14-/- cells pex2-pex10-5 1287642 n/a 1287646 unspecified role unspecified role prey prey n/a 1287654 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287650 unspecified role unspecified role prey prey association association false false false Fig. 3A Membrane extraction with 1% Digitonin. Observed in Pex13-/- cells. pex2-pex10-6 1287642 n/a 1287654 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287647 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role prey prey association association false false false Fig. 3A Membrane extraction with 1% Digitonin. Observed in PEX17-/-cells. pex10-pex2 1287642 n/a 1287652 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287647 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey association association false false false Fig. 3B Membrane extraction with 1% Digitonin. Observed in wild-type, PEX1-/- and PEX4-/-cells. pex10-pex12-3 1287642 n/a 1287646 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 3B Membrane extraction with 1% Digitonin. Observed in PEX5-/-cells. pex10-pex12-4 1287642 n/a 1287646 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287651 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey association association false false false Fig. 3B Membrane extraction with 1% Digitonin. Observed in PEX17-/-cells. pex10-pex12-5 1287642 n/a 1287650 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287646 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3B Membrane extraction with 1% Digitonin. Observed in PEX8-/- and PEX13-/- cells. pex12-pex10 1287642 n/a 1287647 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287651 unspecified role unspecified role prey prey association association false false false Fig. 3C Membrane extraction with 1% Digitonin. Observed in wild-type, PEX1-/- and PEX4-/-cells. pex12-pex10-1 1287642 n/a 1287651 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287648 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey association association false false false Fig. 3C Membrane extraction with 1% Digitonin. Observed in PEX5-/-cells. pex12-pex10-2 1287642 n/a 1287647 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287651 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey association association false false false Fig. 3C Membrane extraction with 1% Digitonin. Observed in PEX17-/-cells. pex12-pex10-3 1287642 n/a 1287646 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287650 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey association association false false false Fig. 3C Membrane extraction with 1% Digitonin. Observed in PEX14-/-cells. pex12-pex10-4 1287642 n/a 1287646 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287650 unspecified role unspecified role prey prey association association false false false Fig. 3C Membrane extraction with 1% Digitonin. Observed in PEX8-/- and PEX13-/-cells. pex14-pex10-3 1287642 n/a 1287646 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287652 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey association association false false false Fig. 4A Membrane extraction with Digitonin pex14-pex10-7 1287642 n/a 1287652 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287647 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey association association false false false Fig. 1A Membrane extraction with Digitonin pex14-pex10-6 1287642 n/a 1287648 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287652 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey association association false false false Fig. 4A Membrane extraction with Digitonin. Observed in PEX8-/- cells pex2-pex10-10 1287642 n/a 1287650 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287654 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287652 unspecified role unspecified role prey prey association association false false false Fig. 4A Membrane extraction with 1% Digitonin. pex2-pex10-9 1287642 n/a 1287650 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287654 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 4A Membrane extraction with 1% Digitonin. Observed in PEX8-/- cells. pex8-pex10-2 1287642 n/a 1287652 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287653 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287650 unspecified role unspecified role prey prey association association false false false Fig. 5D Membrane extraction with 1% Digitonin. Observed in wild-type cells. pex8-pex10-3 1287642 n/a 1287653 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287646 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey association association false false false Fig. 5D Membrane extraction with 1% Digitonin. Observed in PEX17-/- cells. pex8-pex10-4 1287642 n/a 1287647 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey n/a 1287653 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 5D Membrane extraction with 1% Digitonin. Observed in PEX2-/-, PEX10-/- and PEX12-/- cells. pex14-pex10-2 1287643 n/a 1287654 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287653 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey association association false false false Figs. 1A, 1B, 5A Membrane extraction with 1% Digitonin In one experiment, Prohibitin was also observed to be present but could not be ascribed to a single gene product. The presence of PEX17 was confirmed by immunostaining. pex2-pex10-1 1287643 n/a 1287652 unspecified role unspecified role prey prey n/a 1287654 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287651 unspecified role unspecified role prey prey n/a 1287653 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287650 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey association association false false false Figs. 1C Membrane extraction with 1% Digitonin. Presnce of PEX17 confirmed by western blot. pex8-pex10-1 1287643 n/a 1287650 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287654 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287651 unspecified role unspecified role prey prey n/a 1287653 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287646 unspecified role unspecified role prey prey association association false false false Fig. 5A Membrane extraction with 1% Digitonin. Presence of PEX17 confrimed by western blot. pex2-pex10-2 1287643 n/a 1287654 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1287651 unspecified role unspecified role prey prey n/a 1287650 unspecified role unspecified role prey prey n/a 1287653 unspecified role unspecified role prey prey n/a 1287648 unspecified role unspecified role prey prey n/a 1287647 unspecified role unspecified role prey prey n/a 1287646 unspecified role unspecified role prey prey n/a 1287652 unspecified role unspecified role prey prey association association false false false Figs. 5A,5B Membrane extraction with 1% Digitonin. Presnce of PEX17 confirmed by western blot. pex14-pex17-3 1287644 n/a 1287648 unspecified role unspecified role neutral component neutral component n/a 1287651 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 2A, 4B Observed in wild type and PEX8-/- cells pex10-pex12 1287644 n/a 1287646 unspecified role unspecified role neutral component neutral component n/a 1287651 unspecified role unspecified role neutral component neutral component n/a 1287648 unspecified role unspecified role neutral component neutral component n/a 1287650 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 2A pex10-pex12-1 1287644 n/a 1287646 unspecified role unspecified role neutral component neutral component n/a 1287650 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 2A pex14-pex17-4 1287645 n/a 1287648 unspecified role unspecified role neutral component neutral component n/a 1287651 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 2B, 4B Observed in wild-type and PEX8-/- cells pex10-pex12-6 1287645 n/a 1287646 unspecified role unspecified role neutral component neutral component n/a 1287650 unspecified role unspecified role neutral component neutral component physical association physical association false false false Figs. 2B, 4C Observed in wild-type and PEX8-/- cells.