UniProt UniProt http://www.uniprot.org koch-2003-1 ADP-ribosylation factor-dependent phospholipase D2 activation is required for agonist-induced mu-opioid receptor endocytosis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Koch T., Brandenburg L.O., Schulz S., Liang Y., Klein J., Hollt V. Thomas.Koch@Medizin.Uni-Magdeburg.de Rat brain MATCHMAKER cDNA library (Clontech). J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation koch-2003-2 ADP-ribosylation factor-dependent phospholipase D2 activation is required for agonist-induced mu-opioid receptor endocytosis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Koch T., Brandenburg L.O., Schulz S., Liang Y., Klein J., Hollt V. Thomas.Koch@Medizin.Uni-Magdeburg.de Rat brain MATCHMAKER cDNA library (Clontech). J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation koch-2003-3 ADP-ribosylation factor-dependent phospholipase D2 activation is required for agonist-induced mu-opioid receptor endocytosis. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Koch T., Brandenburg L.O., Schulz S., Liang Y., Klein J., Hollt V. Thomas.Koch@Medizin.Uni-Magdeburg.de Rat brain MATCHMAKER cDNA library (Clontech). J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation oprm_rat Mu-type opioid receptor Oprm1 Ror-b Opioid receptor B protein protein rat Rattus norvegicus (Rat) Rat MDSSTGPGNTSDCSDPLAQASCSPAPGSWLNLSHVDGNQSDPCGLNRTGLGGNDSLCPQTGSPSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTIEQQNSTRVRQNTREHPSTANTVDRTNHQLENLEAETAPLP pld2_rat Phospholipase D2 Pld2 Choline phosphatase 2 PLD1C Phosphatidylcholine-hydrolyzing phospholipase D2 protein protein rat Rattus norvegicus (Rat) Rat MTVTQTDLFPYGDYLNSSQLHMEPDVVDTLKEGEDPADRMHPFLAIYDLQPLRAHPLVFAPGVPVIAQVVGTERYTSGSKVGTCTLYSVRLTHGDFTWTTKKKFRHFQELHRDLQRHKVLMSLLNLARFAAAHSPAREAANENIPSLPRGGSEGSARHTASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGSKGLEGVIRKRSGGHRVPGFTCCGRDQVCYRWSKRWLVVKDSFLLYMRPETGAISFVQLFDPGFEVQVGKRSTEARYGVRIDTSHRSLILKCSSYRQARWWGQEITELAQGPGRDFLQLHQHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAREEIFITDWWLSPEIYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRTLMLLHPNIKVMRHPDLVTLWAHHEKLLVVDQAVAFLGGLDLAYGRWDDVQYRLTDLGDPSESADSQTPTPGSDPAATPDLSHNHFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGVAARDLARHFIQRWNFTKTIKARYKIPQYPYLLPKSASTANHLPFIIPGGQCATVQVLRSVDRWSAGTLESSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHEQGQCFRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYMSICGLRTHGELGGHPISELIYIHSKLLIADDRTVIIGSANINDRSLLGKRDSELAILIKDTEMEPSLMDGVEYQAGRFALSLRGRCFSVILGANTWPDLDLRDPVCDDFFQLWQETAENNATIYEQIFRCLPSNATRSLRALREYVAVESLATVSPSLAQSELAHIRGHLVHFPLKFLEDESLLPHWGAKRGMIPLEVWT arfg1_human ADP-ribosylation factor GTPase-activating protein 1 ADP-ribosylation factor 1 GTPase-activating protein ARF1-directed GTPase-activating protein ARFGAP1 ARF1GAP protein protein human Homo sapiens Human MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW oprm1-pld2 1295107 n/a 1295110 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1295111 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1295117 1295115 physical association physical association false false false no figure oprm1-pld2-1 1295108 n/a 1295110 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1295111 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure oprm1-arfgap1 1295109 n/a 1295112 unspecified role unspecified role prey prey n/a 1295111 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n/a 1295110 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false 2a DAMGO; increases association of ARFGAP1/ARF1