mbinfo MBInfo http://www.mechanobio.info/ http://www.mechanobio.info/ martinez_quiles-2001-2 WIP regulates N-WASP-mediated actin polymerization and filopodium formation. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation martinez_quiles-2001-1 WIP regulates N-WASP-mediated actin polymerization and filopodium formation. mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation martinez_quiles-2001-3 WIP regulates N-WASP-mediated actin polymerization and filopodium formation. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. Could not curate interactions from fig 1b, 2 and 3b since the source of recombinant WIP and GST-CDC42 could not be traced. Interactions involving F-actin could not be curated since the number of actin monomers in the filament is not definite. Interaction detection method: GST pull down. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation martinez_quiles-2001-4 WIP regulates N-WASP-mediated actin polymerization and filopodium formation. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Martinez-Quiles N., Rohatgi R., Anton I.M., Medina M., Saville S.P., Miki H., Yamaguchi H., Takenawa T., Hartwig J.H., Geha R.S., Ramesh N. The source of WIP and N-WASP (both endogenous) inferred from the cells used (NIH3T3). raif.geha@tch.harvard.edu Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation wipf1_human WAS/WASL-interacting protein family member 1 WIPF1 WIP Wiskott-Aldrich syndrome protein-interacting protein Protein PRPL-2 WASPIP protein protein human Homo sapiens Human MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPLLPPGGRSTSAKPFSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSPHNRGSPPVPGGPRQPSPGPTPPPFPGNRGTALGGGSIRQSPLSSSSPFSNRPPLPPTPSRALDDKPPPPPPPVGNRPSIHREAVPPPPPQNNKPPVPSTPRPSASSQAPPPPPPPSRPGPPPLPPSSSGNDETPRLPQRNLSLSSSTPPLPSPGRSGPLPPPPSERPPPPVRDPPGRSGPLPPPPPVSRNGSTSRALPATPQLPSRSGVDSPRSGPRPPLPPDRPSAGAPPPPPPSTSIRNGFQDSPCEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNRRERGAPPLPPIPR wasl_human Neural Wiskott-Aldrich syndrome protein WASL protein protein human Homo sapiens Human MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDEEDFEDDDEWED wipf1_mouse WAS/WASL-interacting protein family member 1 Wiskott-Aldrich syndrome protein-interacting protein Wipf1 Wip Waspip protein protein mouse Mus musculus Mouse MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGASAGGYGGGGGGGGGGGGGGGGSGGNFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPILPPGGRATSAKPFSPPSGPGRFPAPSPGHRSGPPEPPRNRMPPPRPDVGSKPDSLPPPVPNTPRPVPSSLHNRGSPAGLGAPRPPFPGNRGAAFGAGSARQNPSGSSSPFPRPPLPPTPSRALDDKPPPPPPPVGNRPSMHREAVPPPPSQTSKPPVPSTPRPGLGSQAPPPPPPPSRPGPPPLPPASNDEIPRLPQRNLSLTSSAPPLPSPGRSGPLPPPPSERPPPPVRDPPGRSGPLPPPPPINRNGSTARALPATPQLPSRSGMDSPRSGPRPPLPPDRPGAGAPPPPPPSTSVRNGFQDSSCEDEWESRFYFHPISDLPPPEPYVPTTKTYPSKLARNESRSGSNRRERGAPPLPPIPR wasl_mouse Neural Wiskott-Aldrich syndrome protein Wasl protein protein mouse Mus musculus Mouse MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQLYAADRNCMWAKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDAPNGPNLPMATVDIKNPEITTNRFYGSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPPPPNRMYPPPPPALPSSAPSGPPPPPPPSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGDHQVPAPSGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFEDDDEWED acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF wasl_rat Neural Wiskott-Aldrich syndrome protein Wasl protein protein rat Rattus norvegicus (Rat) Rat MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDAPNGPNLPMATVDIKNPEITTNRFYSSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPPPPNRMYPPPPPALPSSAPSGPPPPPPLSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGDHQVPASSGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFQDDDEWED q2ydc4_human WIPF1 protein protein human Homo sapiens Human MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPLLPPGGRSTSAKPFSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSLHNRGSPPVPGGPRQPSPGPTPPPFPGNRGTALGGGSIRQSPLSSSSPFSNRPPLPPTPSRALDDKPPPPPPPVGNRPSIHREAVPPPPPQNNKPPVPSTPRPSASSQAPPPPPPPSRPGPPPLPPSSSGNDETPRLPQRNLSLSSSTPPLPSPGRSGPLPPPPSERPPPPVRDPPGRSGMTRRGVSCFQNPACPNVPIILSVKIV wasl-wipf1-1 1299093 n/a 1299097 unspecified role unspecified role prey prey n/a 1299098 unspecified role unspecified role bait bait n-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false This region includes the WH1 domain of N-WASP. physical association physical association false false false Fig 1d. wasl-wipf1 1299093 n/a 1299098 unspecified role unspecified role bait bait n/a 1299097 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1e. wipf1-wasl-1 1299094 n/a 1299099 unspecified role unspecified role bait bait n/a 1299100 unspecified role unspecified role prey prey physical association physical association false false false Fig 1a. wipf1-acta1 1299095 n/a 1299101 unspecified role unspecified role prey prey rabit Oryctolagus cuniculus (Rabbit) Rabbit n/a 1299097 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 3a. wipf1-wasl-3 1299096 n/a 1299102 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a 1299103 unspecified role unspecified role bait bait region uncategorized protein modification uncategorized protein modification undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction false false false Fig 1c.