MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint sim_es-2011-1 Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis. in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Simões I., Faro R., Bur D., Kay J., Faro C. isimoes@biocant.pt FEBS J. (1742-464X) 2011 a1s3n8_sheam Sama_0787 protein protein sheam Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) MSKRIIELKLTNLLAEGGYTACLRLGSEAQSVNLIIDTGSSTLVVHESGYKAGNDKELKPTTLAQEVNYGLGGWLGAVVHTSIHAGELAMADTPLALVHAEAEHTFLDADGIWGMAYHPLNRGYDLTTYLTSHGIDPASTFPWPFPDEAHPLVKQQLDDFKALLPQLPEEDVATCFTLMEERGLCANRFTLITRRSSIHVTAPNACANTLAADPLNQGLLILGSHAADAAPNHGCAVDLKVVHDRYYNVNLKAVRWQNDTDIALPTAQAAHLCRGSSNAIVDSGASLICLPAVAFTPLMQRLWARLPDSKQLTSPFMGDISQIMAAQKQGIDSSLLNLDDWPRLEFVFEGANGENVCLGVDAHHYWQVNAPDYGKAVFKLMGQLPHWPAQSILGLPLFNPYRVVFRRDLGEQGIVRFIPHPPLSQGPVSQSPLNQNPINQSKAMP mint ins_bovin Insulin INS protein protein bovin Bos taurus (Bovine) Bovine MALWTRLRPLLALLALWPPPPARAFVNQHLCGSHLVEALYLVCGERGFFYTPKARREVEGPQVGALELAGGPGAGGLEGPPQKRGIVEQCCASVCSLYQLENYCN cdr1 peptide CDR1 Peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KLHPEVLFVLEK mint synthetic fluorogeni KKPAEFFALK peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KKPAEFFALK mint a1s3n8-ins 1167116 a1s3n8_sheam Shewasin A 1167117 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ins_bovin Oxidized Insulin B chain 1167118 ms participant Identification by mass spectrometry ms participant enzyme target enzyme target substrate substrate neutral component neutral component purified purified other modification other modification uncategorized protein modification uncategorized protein modification modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false resulting-ptm binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false cleavage cleavage reaction cleavage false false false t1 mint a1s3n8-ins-1 1167116 a1s3n8_sheam Shewasin A 1167117 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ins_bovin Oxidized Insulin B chain 1167118 ms participant Identification by mass spectrometry ms participant enzyme target enzyme target substrate substrate neutral component neutral component purified purified other modification other modification uncategorized protein modification uncategorized protein modification modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false resulting-ptm binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false cleavage cleavage reaction cleavage false false false t1 mint a1s3n8-cdr1_peptide 1167116 a1s3n8_sheam Shewasin A 1167117 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cdr1 peptide CDR1 substrate peptide 1167119 ms participant Identification by mass spectrometry ms participant enzyme target enzyme target substrate substrate neutral component neutral component purified purified other modification other modification uncategorized protein modification uncategorized protein modification modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false resulting-ptm cleavage cleavage reaction cleavage false false false t1 mint a1s3n8-cdr1_peptid-1 1167116 a1s3n8_sheam Shewasin A 1167117 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cdr1 peptide CDR1 substrate peptide 1167119 ms participant Identification by mass spectrometry ms participant enzyme target enzyme target substrate substrate neutral component neutral component purified purified other modification other modification uncategorized protein modification uncategorized protein modification modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false resulting-ptm cleavage cleavage reaction cleavage false false false t1 mint a1s3n8-synthetic_flu 1167116 a1s3n8_sheam Shewasin A 1167117 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false A1S3N8:p.Asp37Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false synthetic fluorogeni Typical peptide 1167120 ms participant Identification by mass spectrometry ms participant enzyme target enzyme target substrate substrate neutral component neutral component purified purified other modification other modification uncategorized protein modification uncategorized protein modification modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false resulting-ptm cleavage cleavage reaction cleavage false false false mint t1 f4 a1s3n8-synthetic_f-1 1167116 synthetic fluorogeni Typical peptide 1167120 ms participant Identification by mass spectrometry ms participant enzyme target enzyme target substrate substrate neutral component neutral component purified purified other modification other modification uncategorized protein modification uncategorized protein modification modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false resulting-ptm a1s3n8_sheam Shewasin A 1167117 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false A1S3N8:p.Asp37Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false cleavage cleavage reaction cleavage false false false mint t1 f4