InnateDB InnateDB yuk-2011-1 The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr Nat. Immunol. (1529-2908) 2011 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity yuk-2011-2 The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. mouse-bmm mouse bone marrow derived macrophages bmm bone marrow derived macrophages Bone marrow derived macrophages (BMM) were generated in vitro from precursor cells harvested from the femurs of C57BL/6 mice (7�8 weeks) by proliferation and differentiation in the presence of L-cell conditioned medium. as a source of CSF-1 confocal microscopy confocal microscopy immunostaining immunostaining Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr Nat. Immunol. (1529-2908) 2011 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity yuk-2011-3 The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. mouse-bmm mouse bone marrow derived macrophages bmm bone marrow derived macrophages Bone marrow derived macrophages (BMM) were generated in vitro from precursor cells harvested from the femurs of C57BL/6 mice (7�8 weeks) by proliferation and differentiation in the presence of L-cell conditioned medium. as a source of CSF-1 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr Nat. Immunol. (1529-2908) 2011 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity yuk-2011-4 The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yuk J.M., Shin D.M., Lee H.M., Kim J.J., Kim S.W., Jin H.S., Yang C.S., Park K.A., Chanda D., Kim D.K., Huang S.M., Lee S.K., Lee C.H., Kim J.M., Song C.H., Lee S.Y., Hur G.M., Moore D.D., Choi H.S., Jo E.K. hsc@chonnam.ac.kr Nat. Immunol. (1529-2908) 2011 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity tf65_mouse Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit Rela Nfkb3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein mouse Mus musculus Mouse MDDLFPLIFPSEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGYYEADLCPDRSIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFHVPIEEQRGDYDLNAVRLCFQVTVRDPAGRPLLLTPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFNGPTEPRPPTRRIAVPTRNSTSVPKPAPQPYTFPASLSTINFDEFSPMLLPSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTPGPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSEFQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDEDFSSIADMDFSALLSQISS nr0b2_mouse Nuclear receptor subfamily 0 group B member 2 Nr0b2 Shp Orphan nuclear receptor SHP Small heterodimer partner protein protein mouse Mus musculus Mouse MSSGQSGVCPCQGSAGRPTILYALLSPSPRTRPVAPASHSHCLCQQQRPVRLCAPHRTCREALDVLAKTVAFLRNLPSFCHLPHEDQRRLLECCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEASSGTQGAQPSDRPQPSLAAVQWLQRCLESFWSLELGPKEYAYLKGTILFNPDVPGLRASCHIAHLQQEAHWALCEVLEPWYPASQGRLARILLMASTLKNIPGTLLVDLFFRPIMGDVDITELLEDMLLLR traf6_mouse TNF receptor-associated factor 6 RING-type E3 ubiquitin transferase TRAF6 Traf6 E3 ubiquitin-protein ligase TRAF6 protein protein mouse Mus musculus Mouse MSLLNCENSCGSSQSSSDCCAAMAASCSAAVKDDSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYDLDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSRGCRPEDPNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDKVAEMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIKDDTLLVRCEVSTRFDMGGLRKEGFQPRSTDAGV nr0b2_human Nuclear receptor subfamily 0 group B member 2 NR0B2 SHP Orphan nuclear receptor SHP Small heterodimer partner protein protein human Homo sapiens Human MSTSQPGACPCQGAASRPAILYALLSSSLKAVPRPRSRCLCRQHRPVQLCAPHRTCREALDVLAKTVAFLRNLPSFWQLPPQDQRRLLQGCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEPSSSGGSGQLPDRPQPSLAAVQWLQCCLESFWSLELSPKEYACLKGTILFNPDVPGLQAASHIGHLQQEAHWVLCEVLEPWCPAAQGRLTRVLLTASTLKSIPTSLLGDLFFRPIIGDVDIAGLLGDMLLLR nr0b2-rela-1 1170201 n/a 1170205 unspecified role unspecified role prey prey n/a 1170206 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4E, 7H, S19 LPS or MSP nr0b2-traf6 1170201 n/a 1170207 unspecified role unspecified role prey prey n/a 1170206 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5D LPS traf6-nr0b2 1170201 n/a 1170206 unspecified role unspecified role prey prey n/a 1170207 unspecified role unspecified role bait bait physical association physical association false false false Fig. S7 LPS nr0b2-rela-4 1170201 n/a 1170205 unspecified role unspecified role prey prey n/a 1170207 unspecified role unspecified role prey prey n/a 1170206 unspecified role unspecified role bait bait association association false false false Fig. 5F nr0b2-rela-3 1170202 n/a 1170206 unspecified role unspecified role neutral component neutral component n/a 1170205 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 4F, 4G LPS nr0b2-tf65 1170202 n/a 1170205 unspecified role unspecified role neutral component neutral component n/a 1170208 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 4F, 4G LPS nr0b2-traf6-1 1170202 n/a 1170207 unspecified role unspecified role neutral component neutral component n/a 1170206 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 5E LPS traf6-nr0b2-1 1170203 n/a 1170206 unspecified role unspecified role prey prey n/a 1170207 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5B, 5C LPS nr0b2-traf6-2 1170204 n/a 1170207 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 1170208 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5G