IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ liu-2011d-2 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Liu W., Wen W., Wei Z., Yu J., Ye F., Liu C.H., Hardie R.C., Zhang M. mzhang@ust.hk Cell (0092-8674) 2011 Only protein-protein interactions imex curation liu-2011d-4 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Liu W., Wen W., Wei Z., Yu J., Ye F., Liu C.H., Hardie R.C., Zhang M. mzhang@ust.hk Cell (0092-8674) 2011 Only protein-protein interactions imex curation liu-2011d-3 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Liu W., Wen W., Wei Z., Yu J., Ye F., Liu C.H., Hardie R.C., Zhang M. mzhang@ust.hk Cell (0092-8674) 2011 Only protein-protein interactions imex curation liu-2011d-7 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Liu W., Wen W., Wei Z., Yu J., Ye F., Liu C.H., Hardie R.C., Zhang M. mzhang@ust.hk Cell (0092-8674) 2011 Only protein-protein interactions imex curation liu-2011d-1 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Liu W., Wen W., Wei Z., Yu J., Ye F., Liu C.H., Hardie R.C., Zhang M. mzhang@ust.hk Cell (0092-8674) 2011 Only protein-protein interactions imex curation liu-2011d-6 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Liu W., Wen W., Wei Z., Yu J., Ye F., Liu C.H., Hardie R.C., Zhang M. mzhang@ust.hk Cell (0092-8674) 2011 Only protein-protein interactions imex curation liu-2011d-5 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Liu W., Wen W., Wei Z., Yu J., Ye F., Liu C.H., Hardie R.C., Zhang M. mzhang@ust.hk Cell (0092-8674) 2011 Only protein-protein interactions imex curation liu-2011d-8 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liu W., Wen W., Wei Z., Yu J., Ye F., Liu C.H., Hardie R.C., Zhang M. mzhang@ust.hk Cell (0092-8674) 2011 Only protein-protein interactions imex curation liu-2011d-9 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Liu W., Wen W., Wei Z., Yu J., Ye F., Liu C.H., Hardie R.C., Zhang M. mzhang@ust.hk Cell (0092-8674) 2011 Only protein-protein interactions imex curation liu-2011d-10 The INAD Scaffold Is a Dynamic, Redox-Regulated Modulator of Signaling in the Drosophila Eye. in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Liu W., Wen W., Wei Z., Yu J., Ye F., Liu C.H., Hardie R.C., Zhang M. mzhang@ust.hk Cell (0092-8674) 2011 Only protein-protein interactions imex curation cspg4_rat Chondroitin sulfate proteoglycan 4 Cspg4 Ng2 Chondroitin sulfate proteoglycan NG2 HSN tumor-specific antigen protein protein rat Rattus norvegicus (Rat) Rat MLLSPGHPLSAPALALILTLALLVRSTAPASFFGENHLEVPVPSALTRVDLLLQFSTSQPEALLLLAAGQTDHLLLQLQSGHLQVRLALGQNELSLQTPADTVLSDSTTHTVVLTVSNSWAVLSVDGVLNTSAPIPKASHLKVPYGLFVGSSGSLDLPYLKGISRPLRGCLHSAILNGRNLLRPLTPDVHEGCAEEFSAGDEVGLGFSGPHSLAAFPAWSTREEGTLEFTLTTRSQQAPLAFQAGDKRGNFIYVDIFEGHLRAVVEKGQGTMLLRNSVPVADGQPHEVSVHIDVHRLEISVDQYPTRTFNRGVLSYLEPRGSLLLGGLDTEASRHLQEHRLGLTPGAANISLVGCIEDFSVNGRRLGLRDAWLTRDMAAGCRPEEDEYEEEVYGPFEAFSTLAPEAWPVMDLPEPCVPEPGLPAVFANFTQLLTISPLVVAEGGTAWLEWRHVQPTLDLTEAELRKSQVLFSVSQGARHGELELDIPGAQTRKMFTLLDVVNRKARFVHDGSEDTSDQLMLEVSVTSRAPVPSCLRRGQIYILPIQVNPVNDPPRIVFPHGSLMVILEHTQKPLGPEIFQAYDPDSACEGLTIQLLGVSASVPVEHRDQPGEPVTEFSCRDLEAGNIVYVHRGGPAQDLTFRVSDGMQASGPATLKVVAVRPAIQILHNTGLRLAQGSAAAILPANLSVETNAVGQDVSVLFRVTGTLQFGELQKQGAGGVEGTEWWDTLAFHQRDVEQGRVRYLSTDPQHHTQDTVEDLTLEVQVGQETLSNLSFPVTIQRATVWMLQLEPLHTQNPHQETLTSAHLEASLEEEGEGGPYPHIFHYELVQAPRRGNLLLQGTRLSDGQSFSQSDLQAGRVTYRATTRTSEAAEDSFRFRVTSPPHFSPLYTFPIHIGGDPNAPVLTNVLLMVPEGGEGVLSADHLFVKSLNSASYLYEVMEQPHHGSLAWRDPKGRATPVTSFTNEDLLHGRLVYQHDDSETIEDDIPFVATRQGEGSGDMAWEEVRGVFRVAIQPVNDHAPVQTISRVFHVARGGQRLLTTDDVAFSDADSGFSDAQLVLTRKDLLFGSIVAMEEPTRPIYRFTQEDLRKKQVLFVHSGADHGWLQLQVSDGQHQATAMLEVQASEPYLHVANSSSLVVPQGGQGTIDTAVLHLDTNLDIRSGNEVHYHVTAGPHWGQLLRDGQSVTSFSQRDLLDGAILYSHNGSLSPQDTLALSVAAGPVHTSTVLQVTIALEGPLAPLQLVQHKRIYVFQGEAAEIRRDQLEVVQEAVLPADIMFSLRSPPNAGYLVMVSHGASADGPPSLDPVQRFSQEAINSGRVLYLHSRPGAWSDSFSLDVASGLGDPLEGISVELEVLPTVIPLDVQNFSVPEGGTRTLAPPLIQITGPYLGTLPGLVLQVLEPPQHGALQKEDRPQDGTLSTFSWREVEEQLIRYVHDGSETQTDGFILLANASEMDRQSQPMAFTITILPVNDQPPVITTNTGLQIWEGAIVPIPPEALRGIDSDSGPEDLVYTIEQPSNGRIALRVAPDAEAHRFTQAQLDSGLVLFSHRGALEGGFHFDLSDGVHTSPGHFFRVVAQKQVLLSLEGSRKLTVCPESVQPLSSQSLSASSSTGSDPRHLLYQVVRGPQLGRLLHAQQGSAEEALVNFTQAEVNAGNILYEHEISSEPFWEAHDTIGLLLSSSPARDLAATLAVTVSFDAACPQRPSRLWRNKGLWVPEGQRAKITVAALDAANLLASVPASQRGRHDVLFQITQFPTRGQLLVSEEPLHARRPHFLQSELTAGQLVYAHGGGGTQQDGFRFRAHLQGPTGASVAGPQTSEAFVITVRDVNERPPQPQASIPLRITRGSRAPVSRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLAGDNTGPVTHFTQADVDAGRLAFVANGSSVAGVFQLSMSDGASPPIPMSLAVDVLPSTIEVQLRAPLEVPQALGRSSLSRQQLQVISDREEPDVAYRLTQGPLYGQVLVGGQPASAFSQLQVDQGDVVFAFTNFSSSQDHFKVLALARGVNASATVNVTVQALLHVWAGGPWPQGTTLRLDPTVLDASELANRTGSMPRFRLLEGPRYGRVVRVSQGRAESRTNQLVEDFTQQDLEEGRLGLEVGRPEGRSTGPTGDRLTLELQATGVPPAVALLDFATEPYHAAKFYKVTLLSVPEAARTETEKTGKSTPTGQPGQAASSPMPTVAKSGFLGFLEANMFSVIIPVCLVLLLLALILPLLFYLRKRNKTGKHDVQVLTAKPRNGLAGDTETFRKVEPGQAIPLTTVPGQGPPPGGQPDPELLQFCRTPNPALRNGQYWV inad_drome Inactivation-no-after-potential D protein inaD CG3504 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVQFLGKQGTAGELIHMVTLDKTGKKSFGICIVRGEVKDSPNTKTTGIFIKGIVPDSPAHLCGRLKVGDRILSLNGKDVRNSTEQAVIDLIKEADFKIELEIQTFDKSDEQQAKSDPRSNGYMQAKNKFNQEQTTNNNASGGQGMGQGQGQGQGMAGMNRQQSMQKRNTTFTASMRQKHSNYADEDDEDTRDMTGRIRTEAGYEIDRASAGNCKLNKQEKDRDKEQEDEFGYTMAKINKRYNMMKDLRRIEVQRDASKPLGLALAGHKDRQKMACFVAGVDPNGALGSVDIKPGDEIVEVNGNVLKNRCHLNASAVFKNVDGDKLVMITSRRKPNDEGMCVKPIKKFPTASDETKFIFDQFPKARTVQVRKEGFLGIMVIYGKHAEVGSGIFISDLREGSNAELAGVKVGDMLLAVNQDVTLESNYDDATGLLKRAEGVVTMILLTLKSEEAIKAEKAAEEKKKEEAKKEEEKPQEPATAEIKPNKKILIELKVEKKPMGVIVCGGKNNHVTTGCVITHVYPEGQVAADKRLKIFDHICDINGTPIHVGSMTTLKVHQLFHTTYEKAVTLTVFRADPPELEKFNVDLMKKAGKELGLSLSPNEIGCTIADLIQGQYPEIDSKLQRGDIITKFNGDALEGLPFQVCYALFKGANGKVSMEVTRPKPTLRTEAPKA nrx1a_rat Neurexin-1 Nrxn1 Neurexin I-alpha Neurexin-1-alpha protein protein rat Rattus norvegicus (Rat) Rat MGTALVQHGGCCLLCLSLLLLGCWAELGSGLEFPGAEGQWTRFPKWNACCESEMSFQLKTRSARGLVLYFDDEGFCDFLELILTRGGRLQLSFSIFCAEPATLLADTPVNDGAWHSVRIRRQFRNTTLYIDRAEAKWVEVKSKRRDMTVFSGLFVGGLPPELRAAALKLTLASVREREPFKGWIRDVRVNSSQALPVDGSEVKLDEEPPNSGGGSPCEAGDEGDGGVCLNGGVCSVVDDQAVCDCSRTGFRGKDCSQEDNNVEGLAHLMMGDQGKSKEDNNVEGLAHLMMGDQGKEEYIATFKGSEYFCYDLSQNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVNKLHCSVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKQGDPKMKIHGVVAFKCENVATLDPITFETPESFISLPKWNAKKTGSISFDFRTTEPNGLILFSHGKPRHQKDAKHPQMIKVDFFAIEMLDGHLYLLLDMGSGTIKIKALQKKVNDGEWYHVDFQRDGRSGTISVNTLRTPYTAPGESEILDLDDELYLGGLPENKAGLVFPTEVWTALLNYGYVGCIRDLFIDGQSKDIRQMAEIQSTAGVKPSCSRETAKPCLSNPCKNNGMCRDGWNRYVCDCSGTGYLGRSCEREATVLSYDGSMFMKIQLPVVMHTEAEDVSLRFRSQRAYGILMATTSRDSADTLRLELDAGRVKLTVNLDCIRINCNSSKGPETLFAGYNLNDNEWHTVRVVRRGKSLKLTVDDQQAMTGQMAGDHTRLEFHNIETGIITERRYLSSVPSNFIGHLQSLTFNGMAYIDLCKNGDIDYCELNARFGFRNIIADPVTFKTKSSYVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKGYLHYVFDLGNGANLIKGSSNKPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNLDLKSDLYIGGVAKETYKSLPKLVHAKEGFQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDGFSCDCSMTSFSGPLCNDPGTTYIFSKGGGQITHKWPPNDRPSTRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVKFNVGTDDIAIEESNAIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGNNDNERLAIARQRIPYRLGRVVDEWLLDKGRQLTIFNSQATIIIGGKEQGQPFQGQLSGLYYNGLKVLNMAAENDANIAIVGNVRLVGEVPSSMTTESTATAMQSEMSTSIMETTTTLATSTARRGKPPTKEPISQTTDDILVASAECPSDDEDIDPCEPSSGGLANPTRVGGREPYPGSAEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSAQSNGAVVKEKQPSSAKSANKNKKNKDKEYYV nect3_mouse Nectin-3 Nectin3 Nectin cell adhesion molecule 3 Poliovirus receptor-related protein 3 Pvrl3 protein protein mouse Mus musculus Mouse MARTPGPAPLCPGGGKAQLSSAFPPAAGLLLPAPTPPPLLLLLIPLLLFSRLCGALAGSIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSTQTVAVHHPQYGFSVQGDYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAVCVAATGKPVAQIDWEGDLGEMESSTTSFPNETATIVSQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNWFVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTVNYSGVYVCKVSNSLGQRSDQKVIYISDPPTTTTLQPTVQWHSSPADVQDIATEHKKLPFPLSTLATLKDDTIGTIIASVVGGALFLVLVSILAGVFCYRRRRTFRGDYFAKNYIPPSDMQKESQIDVLHQDELDSYPDSVKKENKNPVNNLIRKDYLEEPEKTQWNNVENLTRFERPMDYYEDLKMGMKFVSDERYNESEDGLVSHVDGSVISRREWYV jam1_rat Junctional adhesion molecule A F11r Jam1 Junctional adhesion molecule 1 protein protein rat Rattus norvegicus (Rat) Rat MGTEGKAGSKLLFLFTSMILGSLVQGKGSVYSPQTAVQVPENDSVKLPCIYSGFSSPRVEWKFVQGSTTALVCYNNQITVPYADRVTFSSSGITFSSVTRKDNGEYTCMVSEDGGQNYGEVSIHLTVLVPPSKPTVSIPSSVTIGNRAVLTCSEHDGSPPSEYSWFKDGVPMLTADAKKTRAFINSSYTIDPKSGDLVFDPVSAFDSGEYYCEAQNGYGTAMRSEAVRMEAVELNVGGIVAAVLVTLILLGLLIFGIWFAYSRGYFERTKKGTAPGKKVIYSQPSARSEGEFKQTSSFLV apba1_rat Amyloid-beta A4 precursor protein-binding family A member 1 Neuron-specific X11 protein Neuronal Munc18-1-interacting protein 1 Adapter protein X11alpha Apba1 Mint1 X11 protein protein rat Rattus norvegicus (Rat) Rat MNHLEGSAEVEVADEAPGGEVNESVEADLEHPEVEEEQQPSPPPPAGHAPEDHRAHPAPPPPPPPQEEEEERGECLARSASTESGFHNHTDTAEGDVLAAARDGYEAERAQDADDESAYAVQYRPEAEEYTEQAEAEHAEAAQRRALPNHLHFHSLEHEEAMNAAYSGYVYTHRLFHRAEDEPYAEPYADYGGLQEHVYEEIGDAPELEARDGLRLYERERDEAAAYRQEALGARLHHYDERSDGESDSPEKEAEFAPYPRMDSYEQEEDIDQIVAEVKQSMSSQSLDKAAEDMPEAEQDLERAPTPGGGHPDSPGLPAPAGQQQRVVGTPGGSEVGQRYSKEKRDAISLAIKDIKEAIEEVKTRTIRSPYTPDEPKEPIWVMRQDISPTRDCDDQRPVDGDSPSPGSSSPLGAESSITPLHPGDPTEASTNKESRKSLASFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKTAQKLAKSRKKAPEGESQPMTEVDLFISTQRIKVLNADTQEPMMDHPLRTISYIADIGNIVVLMARRRMPRSNSQENVEASHPSQDAKRQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVFIEKQKGEILGVVIVESGWGSILPTVIIANMMHGGPAEKSGKLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRVKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI q9w2s5_drome X11Lbeta CG1852 CG1861 Dmel\CG32677 Dmint2 X11B x11Lbeta Dmel_CG32677 CG32677 X11LB X11lbeta protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVSTTLTTLAAGQTTSSSNHHHHHHHSHQQQQQQQQQDQSQQQPHQQLPYQQRGYYALNGSLELDCDTSNTNSSSSSNNIGYQQHQQQQQQQQQPPLILSQALLSPSPPLNDQISLLFPKCRPKQQQQQQQQQQQQQQQQQQQLQPAQQHQQHVHPTDVNSSCNSNRSSSPTTAGAVGEPLASVSVSPPPPKATPTSSTPSYYKSVNIITQSPPPHSASSHSSESLSSVTPRISTNPFLCAPLTPPTPPHHHHHHHHQQQHQQKQQQPKQQHQTQQQEQQEEDTTGESVVEHSRSGAAGEVLEYPASFYYHTVGDSRRGPGSYTEMPRKRSSALPASNRQQQLHNGNGSQNPFIKENYWEAPPTRASLLLHSPTEYSEEPQQQQQIQQQQQQQQQQQQQQQQQQQQHLHASARRAYHQQREQVYGINQRPQQQQQQNILRVGRSPVNRSFPQQHQQQQQQQQQQQQQQQQQQQPHQQIPTARSNPCADGLTPLASHYLYGSKSSLDQHPGDWEPREQRKLRLREEREREREREREREREREREHLLLEQQQQLQLQEVQVARDNHLSHLAGQQQQQQQRKRHVYGEERERERDRERDMDRDHRLVNGNADPNESWQHLRVTTETLAEEGGKHGKPADKAQHQMHNTNQSQNSDVNVYREHQLDAWKLERSYTLAVESRHGIIEYEGSPRRIGVTTTSNLGATGAAAASAAADESEPPAAAEGAAAATATSQPQQQVPMATVPPLSSTATSLQKTAARAALAALAQSHPHSSHNILSTLLPHSPNPVNPGQMHAAQQSIQNYPVRPGFPQRIISPPSRDPRSSSPPLQQQQYQQQQQQQLQHQQQHFASLMSSHSNNNSNTNKQPLSSDNNAEDTSNDVSEIGTISDLTTPEAVGLSIGIAAGEMASRASTPPQTAQAPPTAVAGSTANGTGSGNGTATGNATAAGSGLGPLSLHTKSSTFDYLYEFSETRKVLEEFFKCPSTDEQPIMENGSDVDSIDIQYEFHSNFERDEEDLAEEEEAEDDEEVDDDADEENDEAEDEDEPLEQDRDQNYRAQAPPQSGHIDSERLVYSGYGQQPQLQAQLTAQPMSGARRHYSGSPLMRDFDFFLDSTSRSSGERDADQDGLNHNQLLSTSSGHGGGVGVSGGLGVGPGGGHNYRYSPETTDYDSNCGDLDSLSGEMNGGVSTSCSNYAKFYASAMPVLEDGLSSGHTSDTENNNNNQKNLQQSTVNQGGQCPTSLSGSTSIGGSGGISMLMDMKRISTNHSLNSMNNQTTTASTTTDSNGGVGGVGHHLVATLVASPNNEQAKSQCQTQLQSHPPKTLSLDQSPSNHSKVFKNIDPELDSLYSIGVFHRPDTVQMTPPPPAPAPHRKPNNNGCSSGVVNTNGSLAGGSNDVDLLQPSSKHTPLAAAISSTLQRSSPTSTITGNGIGNGSGKTRSAGSNCSSSANGGMPPPIPERTNLVSAVQRSPSPGPNSPVWLSRHLDGGAGKDLLESGSDNHSKNHSADEEDVDTDLETDRLLGHQRLDDQGYYDENKSWDRKPRSLLSKISPKQQIPSTKTRNGYNALLSSTPELPPPIPPKGQSGLGLGLTLGAGNGGKLLDLVGGMVQRSLSRSSSEHSEKSLSKMPISGGDLCAGGVDVVASTVAATSATVVASTPALGSPGNGGGSERSAAEMANPGGAVKLSEQEIGTINGGSVMPLGSGSGTGSGAGSGAGSGTGTNAAVVANSNANNNVGGGGGGGNNSSGSGAGSNSNSGEKKVKKSKSKEGGAVLIEGVLFRAKYLGSTQLVCEGQPTKSTRMMQAEEAVSRIKALAPDGDVQPSTEVDLFISTEKIMVLNTDLKEIMMDHALRTISYIADIGDLVVLMARRRFVPQDIDDAPKPNRTPKMICHVFESDEAQFIAQSIGQAFQVAYMEFLKANGIEDHRFVKEMDYQEVLNSQEIFGDELEIFAKKELQKEVVVPKAKGEILGVVIVESGWGSMLPTVVIANLMSSGAAARCGQLNIGDQLIAINGLSLVGLPLSTCQTYIKNTKNQTVVKFTVVPCAPVVEVKIKRPETKYQLGFSVQNGVICSLLRGGIAERGGVRVGHRIIEINNQSVVAVPHEKIVNLLATSVGEILMKTMPTSMFRLLTGQENPIYI a3rlx3_drome Kon protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSRLRGLGFGWLWLAWLAILQLQTVAAMKVSLFGDGYVSMPLQEAKMSTNIRVKFRTRQENAFLFLAAGRTDYCLLRLESGLISFSYKIERDVVQLRSPKKQKLNDLEWHDVAVQRFENNITLQVDGYIMRKQLPGDLAALNIHFGTFLGGVGDFTAEFLDDVIGFRGCISDVFYNNINIIKRAKDRTSHTTSTGVAWTCSTEFEGSIQDSISFMRNDSYSLMMKESYAMGETLSLQFRTMASAGVIFFNGGFDFILLEIEDQHLKVTFNKAGSLVQFMTNEHISDGKWHRVFLRYNAAIAELILDDATSGYRGTHANETKASINLEKSVFFGGVQEEMRRRLISKGLRINEISFKGCMRDILVNDLPLGFAEMTISRSLALNCLWKYPCVEYNPCLKSGICSQHGVDGFICYCDQSYCIKADFQGPFKIFTETSPELELLYVSPMQLLEGGTAFLSPHFIDIILDLRRYPSLNEQSIIFHVVHQPKYGQLLQYSAEKAIFVPCRTFNLVDLATDKLKYVHNGQENFNDHATLDMQIFGDVHKIPDNILGKHRFLLHANITPINDPPQLRLHSHKILRVIEGIERVLDVDLFNIDDPDSEPGNLIYTILPTQSPQETFGCFIVGGATTSAFSQAEVNVGKVSYLYNSTTAESFSYELQLQVSDGIETSETVYLPVSVHPLELRLVNNTGLIMIHKSSLPISTANLSIGTNAVDDHIDIRYDIVKAPQHGVLQRLRQIDGSWVNVDWFSDSQLLLGHIRYLHSSDFPWQDEFKFIASFGFVTTQTFDFRITFTRLRITSTRPSQISINGSREILLTSDVLSYETTPIGSFARSVIYKITKPTRYGGIYVEGSRKAAKKLDSFTQQDIEKRRIRYQTHHTSYSSFSDHLEFVVSVAECDDVAGVLEINYRPPDELINKLGYQNHEPLQVQEGERALITKNHFGIRFNIYESLQFQVSLSPEHGVICKYDEQTGLTTPVEMFTLEQLFRNDIYYCHDDTESTRDTFELLILSGDETDLQFVSNMEVHIKLVNDNEPYRTAIERVFHVVRNGIRTLNPTVLQYLDADVNTNHTDIHYIHVSSTNGAFYKSGHYIDSFTQDDIANRRIMFQHTGADSGTASFIVTDREHEVNGLLEIRASDPFVSMMATNASIVQEGKFVVLKNKDFILETNLDMKLDEIYYEVIKPPSYGILMYLSRANEGENGTTIYKATNYTSLSNFTHLDIERERLVYWNTEIASMDKVRYRVHIKNITAEGEVMFRIYPSAYWEPLQVKQNHTLYVEESTSVLISRDVLEVVHPNISPGDITFLVTSSPMHGYLEMQSMSFDDEYNCKVFDQSSVNTEKMFYIQAGVNQSTDYFVFDVTNGITWLRQLMIKIVIIPEKLYMHSNIISVVEGKTVQLNPTDIQPYSEYYRGKILEYIVTITPSSGHVLAGNSKVKRFTQKQLEQGSIQYVHNGSENATDSITLVAMARNKESVPFELEFAVVQVNDEEPMMVTNTGLQVWNGGRYVIKNTDLLAQDYDTPPENLTFVVNHIYGGYLARKSAPHQKIEHFTQAQINLEEIYFMHDSNSRRNELSFVVTDGLFNTTTQMLNIEIKPIEILAEHNENLHVFPLTKKQILRDYLHFKCSDEEREIRYNITVPPSLGRIVNEFIDNGFTKEVSEFTQNDVDNGHIFYEHTAVIMEFRTNDSFYFDVVAERSDRLLNQKFNIEISVSSGGLLRFLPVNKLNVDEGGSVPIKLDFSKILEYLKTKAGINNPELFIEAIQKPTHGNIGLGHEFKHMQRYHPSDFFTKKVYYIHDHSDTLEDTILMSVYLTQGNIFLCNLTIPVSINPINDQPFHLVTHLPQMTVVEGENRTITRNDLLTEDADTPPEEIIYDVMSGPTLGVLRKITNDGRPEDLLAFSNQFTQADINNDRIIYVHFGMPQSTTFCFTVSDGQSNPAYEIFTIKIDSIHLQPSAMQAPVKVQQGATTAPMRLDHIGVSTNVHMERLSYNVTNSPLFGIIVYKHQPTLRFTQQQLETSQISYMQTDLNRSNDSFQVSAYVPGTNYVAQVDVVMEVEPVIQINDIVMKEAESSGGKIKLITSLDNDNPLSLKLNKFNPKFVITRMPSTGQIRKIIRSTGLTTDGQSDRSTNIFSYKELRSGVVYFVPHESVEETEADHDSFDYQLLIKTVQPAQATVSIEYRSRVEETETVHLGSAGLSINYLAIGCAIFIVLICLLIVLLILKIRKLRKHKADISKDQPPALPCPPDLTSVSPQHHLHHHHGHHYASSEADSVPATGNSTPLPGFSNIPHCKIIPVESYKHEYPDYDPDEEETDDQCDPQQMMLPQRYNPYHMEHDAWSSSCDMANDFAGYASVPQSISGSVSSPPSAPPTNPLLRRNQYWV trp_drome Transient receptor potential protein trp CG7875 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGSNTESDAEKALGSRLDYDLMMAEEYILSDVEKNFILSCERGDLPGVKKILEEYQGTDKFNINCTDPMNRSALISAIENENFDLMVILLEHNIEVGDALLHAISEEYVEAVEELLQWEETNHKEGQPYSWEAVDRSKSTFTVDITPLILAAHRNNYEILKILLDRGATLPMPHDVKCGCDECVTSQMTDSLRHSQSRINAYRALSASSLIALSSRDPVLTAFQLSWELKRLQAMESEFRAEYTEMRQMVQDFGTSLLDHARTSMELEVMLNFNHEPSHDIWCLGQRQTLERLKLAIRYKQKTFVAHPNVQQLLAAIWYDGLPGFRRKQASQQLMDVVKLGCSFPIYSLKYILAPDSEGAKFMRKPFVKFITHSCSYMFFLMLLGAASLRVVQITFELLAFPWMLTMLEDWRKHERGSLPGPIELAIITYIMALIFEELKSLYSDGLFEYIMDLWNIVDYISNMFYVTWILCRATAWVIVHRDLWFRGIDPYFPREHWHPFDPMLLSEGAFAAGMVFSYLKLVHIFSINPHLGPLQVSLGRMIIDIIKFFFIYTLVLFAFGCGLNQLLWYYAELEKNKCYHLHPDVADFDDQEKACTIWRRFSNLFETSQSLFWASFGLVDLVSFDLAGIKSFTRFWALLMFGSYSVINIIVLLNMLIAMMSNSYQIISERADTEWKFARSQLWMSYFEDGGTIPPPFNLCPNMKMLRKTLGRKRPSRTKSFMRKSMERAQTLHDKVMKLLVRRYITAEQRRRDDYGITEDDIIEVRQDISSLRFELLEIFTNNNWDVPDIEKKSQGVARTTKGKVMERRILKDFQIGFVENLKQEMSESESGRDIFSSLAKVIGRKKTQKGDKDWNAIARKNTFASDPIGSKRSSMQRHSQRSLRRKIIEQANEGLQMNQTQLIEFNPNLGDVTRATRVAYVKFMRKKMAADEVSLADDEGAPNGEGEKKPLDASGSKKSITSGGTGGGASMLAAAALRASVKNVDEKSGADGKPGTMGKPTDDKKAGDDKDKQQPPKDSKPSAGGPKPGDQKPTPGAGAPKPQAAGTISKPGESQKKDAPAPPTKPGDTKPAAPKPGESAKPEAAAKKEESSKTEASKPAATNGAAKSAAPSAPSDAKPDSKLKPGAAGAPEATKATNGASKPDEKKSGPEEPKKAAGDSKPGDDAKDKDKKPGDDKDKKPGDDKDKKPADNNDKKPADDKDKKPGDDKDKKPGDDKDKKPSDDKDKKPADDKDKKPAAAPLKPAIKVGQSSAAAGGERGKSTVTGRMISGWL pipa_drome 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase norpA Phosphoinositide phospholipase C No receptor potential A protein 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta PLC-beta Phosphoinositide phospholipase C-beta CG3620 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTKKYEFDWIIPVPPELTTGCVFDRWFENEKETKENDFERDALFKVDEYGFFLYWKSEGRDGDVIELCQVSDIRAGGTPKDPKILDKVTKKNGTNIPELDKRSLTICSNTDYINITYHHVICPDAATAKSWQKNLRLITHNNRATNVCPRVNLMKHWMRLSYCVEKSGKIPVKTLAKTFASGKTEKLVYTCIKDAGLPDDKNATMTKEQFTFDKFYALYHKVCPRNDIEELFTSITKGKQDFISLEQFIQFMNDKQRDPRMNEILYPLYEEKRCTEIINDYELDEEKKKNVQMSLDGFKRYLMSDENAPVFLDRLDFYMEMDQPLAHYYINSSHNTYLSGRQIGGKSSVEMYRQTLLAGCRCVELDCWNGKGEDEEPIVTHGHAYCTEILFKDCIQAIADCAFVSSEYPVILSFENHCNRAQQYKLAKYCDDFFGDLLLKEPLPDHPLDPGLPLPPPCKLKRKILIKNKRMKPEVEKVELELWLKGELKTDDDPEEDASAGKPPEAAAAPAPAPEAAAAAEGAAEGGGGAEAEAAAANYSGSTTNVHPWLSSMVNYAQPIKFQGFDKAIEKNIAHNMSSFAESAGMNYLKQSSIDFVNYNKRQMSRIYPKGTRADSSNYMPQVFWNAGCQMVSLNFQSSDLPMQLNQGKFEYNGGCGYLLKPDFMRRADKDFDPFADAPVDGVIAAQCSVKVIAGQFLSDKKVGTYVEVDMFGLPSDTVKKEFRTRLVANNGLNPVYNEDPFVFRKVVLPDLAVLRFGVYEESGKILGQRILPLDGLQAGYRHVSLRTEANFPMSLPMLFVNIELKIYVPDGFEDFMAMLSDPRGFAGAAKQQNEQMKALGIEEQSGGAARDAGKAKEEEKKEPPLVFEPVTLESLRQEKGFQKVGKKQIKELDTLRKKHAKERTSVQKTQNAAIDKLIKGKSKDDIRNDANIKNSINDQTKQWTDMIARHRKEEWDMLRQHVQDSQDAMKALMLTVQAAQIKQLEDRHARDIKDLNAKQAKMSADTAKEVQNDKTLKTKNEKDRRLREKRQNNVKRFMEEKKQIGVKQGRAMEKLKLAHSKQIEEFSTDVQKLMDMYKIEEEAYKTQGKTEFYA kpc2_drome Protein kinase C, eye isozyme inaC PKC2 CG6518 Eye-PKC Photoreceptor-specific PKC dPKC53E(EY) Inactivation no after-potential C protein protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAAAAVATPGATVLPPSVPSAAPGAKAPAAGAGKGPGNLLEITGEANIVNYMKNRLRKGAMKRKGLEMVNGHRFGVRFFKNPTYCGHCKDFIWGFGKQGFQCEECRFNIHQKCCKFVVFKCPGKDTDFDADCAKVKHGWISTTYTTPTFCDECGLLLHGVAHQGVKCENCNLNVHHACQETVPPMCGADISEVRGKLLLYVELKGNNLKVDIKEAANLIPMDTNGFSDPYIAVQMHPDRSGRTKKKTKTIQKNLNPVFNETFTFELQPQDRDKRLLIEVWDWDRTSRNDFMGSFSFSLEELQKEPVDGWYKFLSQVEGEHYNIPCVDAFNDIARLRDEVRHDRRPNEKRRMDNKDMPHNMSKRDMIRAADFNFVKVIGKGSFGKVLLAERRGTDELYAVKVLRKDVIIQTDDMELPMNEKKILALSGRPPFLVSMHSCFQTMDRLFFVMEYCKGGDLMYHMQQYGRFKESVAIFYAVEVAIALFFLHERDIIYRDLKLDNILLDGEGHVKLVDFGLSKEGVTERQTTRTFCGTPNYMAPEIVSYDPYSIAADWWSFGVLLFEFMAGQAPFEGDDETTVFRNIKDKKAVFPKHFSVEAMDIITSFLTKKPNNRLGAGRYARQEITTHPFFRNVDWDKAEACEMEPPIKPMIKHRKDISNFDDAFTKEKTDLTPTDKLFMMNLDQNDFIGFSFMNPEFITII kpca_human Protein kinase C alpha type PRKCA PKCA PRKACA protein protein human Homo sapiens Human MADVFPGNDSTASQDVANRFARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVADEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNMELRQKFEKAKLGPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSVCKGLMTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV cspg4-inad 1168585 n/a 1168595 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1168596 unspecified role unspecified role prey prey pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1168611 1168609 direct interaction direct interaction false false false Fig. 1A nrxn1-inad 1168585 n/a 1168597 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1168596 unspecified role unspecified role prey prey pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1168616 1168614 direct interaction direct interaction false false false Fig. 1A pvrl3-inad 1168585 n/a 1168596 unspecified role unspecified role prey prey pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1168598 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) 1168621 1168619 direct interaction direct interaction false false false Fig. 1A f11r-inad 1168585 n/a 1168599 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1168596 unspecified role unspecified role prey prey pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1A apba1-inad 1168585 n/a 1168600 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1168596 unspecified role unspecified role prey prey pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1A apba1-inad-2 1168586 n/a 1168596 unspecified role unspecified role neutral component neutral component pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1168600 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) 1168636 1168634 direct interaction direct interaction false false false Fig. 1A inad-nrxn1 1168586 n/a 1168597 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1168596 unspecified role unspecified role neutral component neutral component pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1168641 1168639 direct interaction direct interaction false false false 1168586 Fig. 1A inad-pvrl3 1168586 n/a 1168596 unspecified role unspecified role neutral component neutral component pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1168598 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) 1168646 1168644 direct interaction direct interaction false false false 1168586 Fig. 1A cspg4-inad-3 1168586 n/a 1168596 unspecified role unspecified role neutral component neutral component pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1168595 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) 1168651 1168649 direct interaction direct interaction false false false 1168586 Fig. 1A f11r-inad-2 1168586 n/a 1168599 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1168596 unspecified role unspecified role neutral component neutral component pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1168656 1168654 direct interaction direct interaction false false false 1168586 Fig. 1A inad-x11lbeta 1168587 n/a 1168596 unspecified role unspecified role neutral component neutral component pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1168601 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1168587 Fig. 1A inad-kon-1 1168587 n/a 1168602 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1168596 unspecified role unspecified role neutral component neutral component pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1168587 Figs. 1A, 1B, 5F, 5G, Supp. Fig. 4A Binding is not affected by a change in pH from 7.5 to 5.8. inad-trp-1 1168587 n/a 1168603 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1168596 unspecified role unspecified role neutral component neutral component pdz4_5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1168587 Fig. 6B inad-norpa 1168587 n/a 1168604 unspecified role unspecified role neutral component neutral component c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1168596 unspecified role unspecified role neutral component neutral component pdz4_5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1168676 1168674 direct interaction direct interaction false false false 1168587 Supp. Fig. 6C norpA will bind to the PDZ5 domain of inad but with a Kd=30uM. The oxidation of PDZ45 abolished its binding to the norpA peptide inac-inad-2 1168588 n/a 1168605 enzyme enzyme neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a 1168596 enzyme target enzyme target substrate substrate neutral component neutral component Q24008:p.Thr513Ala mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false pdz4_5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q24008:p.Thr669Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figs. 5A, 5B Wild type Inad was not phosphorylated by ePKC, however substitution of Thr669 with Ala, a mutation originally designed to mimic non-phosphorylated state of the residue, led to dramatically increased phosphorylation. Thr513Ala in the PDZ45 interface also led to an increase of its phosphorylation prkca-inad-1 1168588 n/a 1168606 enzyme enzyme neutral component neutral component n/a 1168596 enzyme target enzyme target substrate substrate neutral component neutral component pdz2 region sufficient to bind sufficient binding region sufficient to bind range range range range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp. Fig. 5B prkca-inad 1168588 n/a 1168606 enzyme enzyme neutral component neutral component n/a 1168596 enzyme target enzyme target substrate substrate neutral component neutral component pdz2_4_5 region sufficient to bind sufficient binding region sufficient to bind range range range range false range range range range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp. Fig. 5B inac-1 1168588 n/a 1168605 putative self putative self putative self putative self region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction false true false Supp. Fig. 5C kon-inad 1168589 n/a 1168602 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1168596 unspecified role unspecified role prey prey pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1168699 1168697 direct interaction direct interaction false false false Fig. 1A x11lbeta-inad 1168589 n/a 1168601 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1168596 unspecified role unspecified role prey prey pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1168704 1168702 direct interaction direct interaction false false false Fig. 1A inad-kon 1168590 n/a 1168596 unspecified role unspecified role neutral component neutral component pdz5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1168602 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1168710 1168707 direct interaction direct interaction false false false Supp. Figs. 1, 4B inad-trp-1 1168590 n/a 1168596 unspecified role unspecified role neutral component neutral component pdz4_5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1168603 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Fig. 6C cspg4-inad-2 1168591 n/a 1168596 unspecified role unspecified role prey prey pdz4_5 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1168595 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) 1168719 1168717 direct interaction direct interaction false false false Supp. Table 2 2.6A Rworking=19.8% Rfree=26.3% inad-trp-1 1168592 n/a 1168603 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false gb1 tag region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1168596 unspecified role unspecified role neutral component neutral component pdz 3_4_5 region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 6F trp-inad-1 1168593 n/a 1168596 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) n/a 1168603 unspecified role unspecified role bait bait P19334:p.[Val1266Ala;Trp1274Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P19334:p.Val1266Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 6D trp-inad-2 1168594 n/a 1168603 unspecified role unspecified role bait bait gb1 tag region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1168596 unspecified role unspecified role prey prey trp 3_4_5 region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp Fig. 6A