IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chen-2011b-1 CCL18 from Tumor-Associated Macrophages Promotes Breast Cancer Metastasis via PITPNM3. human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Chen J., Yao Y., Gong C., Yu F., Su S., Chen J., Liu B., Deng H., Wang F., Lin L., Yao H., Su F., Anderson KS., Liu Q., Ewen ME., Yao X., Song E. 125I-labeled CCL18 was incubated with 6 X 105 cells resuspended in binding buffer in the presence or absence of unlabeled chemokines. After incubation at room temperature for 30 min, the cells were pelleted through a PBS cushion with 10% sucrose for 1 min at 10,000g. The supernatant was removed and the radioactivity associated with cell pellets was measured using a gamma-counter. yaoxb@ustc.edu.cn, songerwei02@yahoo.com.cn Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation chen-2011b-2 CCL18 from Tumor-Associated Macrophages Promotes Breast Cancer Metastasis via PITPNM3. human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen J., Yao Y., Gong C., Yu F., Su S., Chen J., Liu B., Deng H., Wang F., Lin L., Yao H., Su F., Anderson KS., Liu Q., Ewen ME., Yao X., Song E. 125I-labeled CCL18 was incubated with 6 X 105 cells resuspended in binding buffer in the presence or absence of unlabeled chemokines. After incubation at room temperature for 30 min, the cells were pelleted through a PBS cushion with 10% sucrose for 1 min at 10,000g. The supernatant was removed and the radioactivity associated with cell pellets was measured using a gamma-counter. yaoxb@ustc.edu.cn, songerwei02@yahoo.com.cn Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation chen-2011b-3 CCL18 from Tumor-Associated Macrophages Promotes Breast Cancer Metastasis via PITPNM3. human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Chen J., Yao Y., Gong C., Yu F., Su S., Chen J., Liu B., Deng H., Wang F., Lin L., Yao H., Su F., Anderson KS., Liu Q., Ewen ME., Yao X., Song E. 125I-labeled CCL18 was incubated with 6 X 105 cells resuspended in binding buffer in the presence or absence of unlabeled chemokines. After incubation at room temperature for 30 min, the cells were pelleted through a PBS cushion with 10% sucrose for 1 min at 10,000g. The supernatant was removed and the radioactivity associated with cell pellets was measured using a gamma-counter. yaoxb@ustc.edu.cn, songerwei02@yahoo.com.cn Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation chen-2011b-4 CCL18 from Tumor-Associated Macrophages Promotes Breast Cancer Metastasis via PITPNM3. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. competition binding competition binding predetermined predetermined participant predetermined Chen J., Yao Y., Gong C., Yu F., Su S., Chen J., Liu B., Deng H., Wang F., Lin L., Yao H., Su F., Anderson KS., Liu Q., Ewen ME., Yao X., Song E. 125I-labeled CCL18 was incubated with 6 X 105 cells resuspended in binding buffer in the presence or absence of unlabeled chemokines. After incubation at room temperature for 30 min, the cells were pelleted through a PBS cushion with 10% sucrose for 1 min at 10,000g. The supernatant was removed and the radioactivity associated with cell pellets was measured using a gamma-counter. yaoxb@ustc.edu.cn, songerwei02@yahoo.com.cn Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation ccl18_human C-C motif chemokine 18 CCL18 AMAC1 DCCK1 MIP4 PARC SCYA18 Dendritic cell chemokine 1 Small-inducible cytokine A18 Macrophage inflammatory protein 4 Pulmonary and activation-regulated chemokine CC chemokine PARC Alternative macrophage activation-associated CC chemokine 1 protein protein human Homo sapiens Human MKGLAAALLVLVCTMALCSCAQVGTNKELCCLVYTSWQIPQKFIVDYSETSPQCPKPGVILLTKRGRQICADPNKKWVQKYISDLKLNA pitm3_human Membrane-associated phosphatidylinositol transfer protein 3 PITPNM3 NIR1 Phosphatidylinositol transfer protein, membrane-associated 3 Pyk2 N-terminal domain-interacting receptor 1 protein protein human Homo sapiens Human MAKAGRAGGPPPGGGAPWHLRNVLSDSVESSDDEFFDAREEMAEGKNAILIGMSQWNSNDLVEQIETMGKLDEHQGEGTAPCTSSILQEKQRELYRVSLRRQRFPAQGSIEIHEDSEEGCPQRSCKTHVLLLVLHGGNILDTGAGDPSCKAADIHTFSSVLEKVTRAHFPAALGHILIKFVPCPAICSEAFSLVSHLNPYSHDEGCLSSSQDHVPLAALPLLAISSPQYQDAVATVIERANQVYREFLKSSDGIGFSGQVCLIGDCVGGLLAFDAICYSAGPSGDSPASSSRKGSISSTQDTPVAVEEDCSLASSKRLSKSNIDISSGLEDEEPKRPLPRKQSDSSTYDCEAITQHHAFLSSIHSSVLKDESETPAAGGPQLPEVSLGRFDFDVSDFFLFGSPLGLVLAMRRTVLPGLDGFQVRPACSQVYSFFHCADPSASRLEPLLEPKFHLVPPVSVPRYQRFPLGDGQSLLLADALHTHSPLFLEGSSRDSPPLLDAPASPPQASRFQRPGRRMSEGSSHSESSESSDSMAPVGASRITAKWWGSKRIDYALYCPDVLTAFPTVALPHLFHASYWESTDVVAFILRQVMRYESVNIKESARLDPAALSPANPREKWLRKRTQVKLRNVTANHRANDVIAAEDGPQVLVGRFMYGPLDMVALTGEKVDILVMAEPSSGRWVHLDTEITNSSGRITYNVPRPRRLGVGVYPVKMVVRGDQTCAMSYLTVLPRGMECVVFSIDGSFAASVSIMGSDPKVRPGAVDVVRHWQDLGYMILYITGRPDMQKQRVVSWLSQHNFPQGMIFFSDGLVHDPLRQKAIFLRNLMQECFIKISAAYGSTKDISVYSVLGLPASQIFIVGRPTKKYQTQCQFLSEGYAAHLAALEASHRSRPKKNNSRMILRKGSFGLHAQPEFLRKRNHLRRTMSVQQPDPPAANPKPERAQSQPESDKDHERPLPALSWARGPPKFESVP ccl18-pitpnm3 1154232 n/a 1154236 unspecified role unspecified role bait bait n/a 1154237 unspecified role unspecified role prey prey physical association physical association false false false Fig 4B ccl18-pitpnm3-1 1154233 n/a 1154236 unspecified role unspecified role bait bait n/a 1154237 unspecified role unspecified role prey prey physical association physical association false false false Fig 4D ccl18-pitpnm3-3 1154234 n/a 1154237 unspecified role unspecified role neutral component neutral component n/a 1154236 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 5D ccl18-pitpnm3-5 1154235 n/a 1154237 unspecified role unspecified role neutral component neutral component n/a 1154236 unspecified role unspecified role neutral component neutral component region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 5C CCL3 and CCL4 with low affinity