MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint igarashi-2011-1 Autophosphorylation of heme-regulated eukaryotic initiation factor 2α kinase and the role of the modification in catalysis. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Igarashi J., Sasaki T., Kobayashi N., Yoshioka S., Matsushita M., Shimizu T. jotaro@tagen.tohoku.ac.jp FEBS J. (1742-464X) 2011 igarashi-2011-2 Autophosphorylation of heme-regulated eukaryotic initiation factor 2α kinase and the role of the modification in catalysis. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Igarashi J., Sasaki T., Kobayashi N., Yoshioka S., Matsushita M., Shimizu T. jotaro@tagen.tohoku.ac.jp FEBS J. (1742-464X) 2011 e2ak1_mouse Eukaryotic translation initiation factor 2-alpha kinase 1 Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase Hemin-sensitive initiation factor 2-alpha kinase Heme-regulated inhibitor Heme-controlled repressor Eif2ak1 Hri protein protein mouse Mus musculus Mouse MLGGSSVDGERDTDDDAAGAVAAPPAIDFPAEVSDPKYDESDVPAELQVLKEPLQQPTFPFLVANQLLLVSLLEHLSHVHEPNPLHSKQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCQDNSYMQKIRSREIAFEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSATKTDCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVVQPQDRVPIQLPSLEVLSEQEGDRDQGGVKDNESSSSIVFAELTPEKEKPFGESEVKNENNNLVSYTANLVVRNSSESESSIELQEDGLTDLSVRPVVRHQLPLGHSSELEGNFTSTDESSEGNLNLLGQTEVRYHLMLHIQMQLCELSLWDWITERNKRSREYVDEAACPYVMASVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACADIIQNADWTNRNGKGTRTHTSRVGTCLYASPEQLEGSQYDAKSDMYSLGVILLELFQPFGTEMERATVLTGVRTGRIPESLSKRCPVQAKYIQLLTGRNVSQRPSALQLLQSELFQTTGNVNLTLQMKIIEQEKEIEELKKQLSLLSQDRGLKR if2a_mouse Eukaryotic translation initiation factor 2 subunit 1 Eif2s1 Eif2a Eukaryotic translation initiation factor 2 subunit alpha protein protein mouse Mus musculus Mouse MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTETMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQLERLERENAEVDGDDDAEEMEAKAED eif2ak1-eif2s1-2 1009362 e2ak1_mouse HRI 1009364 predetermined predetermined participant predetermined enzyme enzyme unspecified role unspecified role purified purified Q9Z2R9:p.His120Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q9Z2R9:p.His119Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false if2a_mouse Eif2a 1009365 weight by comassie molecular weight estimation by coomasie staining weight by comassie enzyme target enzyme target substrate substrate neutral component neutral component purified purified phosphorylation phosphorylation reaction phosphorylation false false false homomint mint f3 sf2 f4 sf5 eif2ak1-1 1009362 e2ak1_mouse HRI 1009364 western blot western blot Immuno blot Immuno blot self self self self purified purified phosphorylation phosphorylation reaction phosphorylation false true false mint fs2 true eif2ak1-eif2s1-3 1009362 if2a_mouse Eif2a 1009365 weight by comassie molecular weight estimation by coomasie staining weight by comassie enzyme target enzyme target substrate substrate neutral component neutral component purified purified e2ak1_mouse HRI 1009364 predetermined predetermined participant predetermined enzyme enzyme unspecified role unspecified role purified purified Q9Z2R9:p.Lys196Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false phosphorylation phosphorylation reaction phosphorylation false false false homomint mint f3 sf2 f4 sf5 eif2ak1-eif2s1-4 1009362 e2ak1_mouse HRI 1009364 predetermined predetermined participant predetermined enzyme enzyme unspecified role unspecified role purified purified Q9Z2R9:p.Ser502Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false if2a_mouse Eif2a 1009365 weight by comassie molecular weight estimation by coomasie staining weight by comassie enzyme target enzyme target substrate substrate neutral component neutral component purified purified phosphorylation phosphorylation reaction phosphorylation false false false homomint mint f3 sf2 f4 sf5c eif2ak1-eif2s1-5 1009362 e2ak1_mouse HRI 1009364 predetermined predetermined participant predetermined enzyme enzyme unspecified role unspecified role purified purified Q9Z2R9:p.Thr490Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false if2a_mouse Eif2a 1009365 weight by comassie molecular weight estimation by coomasie staining weight by comassie enzyme target enzyme target substrate substrate neutral component neutral component purified purified phosphorylation phosphorylation reaction phosphorylation false false false homomint mint f3 sf2 f4 sf5a eif2ak1-eif2s1-6 1009362 if2a_mouse Eif2a 1009365 weight by comassie molecular weight estimation by coomasie staining weight by comassie enzyme target enzyme target substrate substrate neutral component neutral component purified purified e2ak1_mouse HRI 1009364 predetermined predetermined participant predetermined enzyme enzyme unspecified role unspecified role purified purified Q9Z2R9:p.Tyr193Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false phosphorylation phosphorylation reaction phosphorylation false false false homomint mint f3 sf2 f4 sf5a eif2ak1-eif2s1-7 1009362 e2ak1_mouse HRI 1009364 predetermined predetermined participant predetermined enzyme enzyme unspecified role unspecified role purified purified Q9Z2R9:p.Thr485Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false if2a_mouse Eif2a 1009365 weight by comassie molecular weight estimation by coomasie staining weight by comassie enzyme target enzyme target substrate substrate neutral component neutral component purified purified phosphorylation phosphorylation reaction phosphorylation false false false homomint mint f3 sf2 f4 sf5a eif2ak1-eif2ak1 1009363 e2ak1_mouse HRI- KD 1009364 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified binding site binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false e2ak1_mouse HRI - NTD 1009364 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false f2 homomint mint