IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ braun-2011-1 The cul4-ddb1(cdt2) ubiquitin ligase inhibits invasion of a boundary-associated antisilencing factor into heterochromatin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Braun S., Garcia JF., Rowley M., Rougemaille M., Shankar S., Madhani HD. hitenmadhani@gmail.com Cell (0092-8674) 2011 Only protein-protein interactions imex curation braun-2011-2 The cul4-ddb1(cdt2) ubiquitin ligase inhibits invasion of a boundary-associated antisilencing factor into heterochromatin. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Braun S., Garcia JF., Rowley M., Rougemaille M., Shankar S., Madhani HD. hitenmadhani@gmail.com Cell (0092-8674) 2011 Only protein-protein interactions imex curation jhd1_schpo Putative JmjC domain-containing histone demethylation protein 1 jhd1 epe1 Heterochromatin-destabilizing protein epe1 [Histone-H3]-lysine-36 demethylase 1 SPCC622.16c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDSWLEYDDIINQDIDIPSNDLSGSGTLCVGVHSSLLENSLNSIDSFISSKEEISWCGNQSTPIATKSHLSCINPQYVNPFDTSPVSVDTEFQDTYLLDAPSFAQPHFSERQSVDKTRSRCLSRNRRRKRHPNLHKNHQRLLGMSFPQDGFRRMPAESVNFSYFRDTGFNEPTIFPSSDTQNTRQLNLSKIATLIGYDCPLALVDVVTQKQIPNKMDMESWVKYMSLEPSKRGRIYDVLSLEVSTTKLAYYVRKPNIVRDLDLVNTVWPPGSFALGEYPHVDTYCLMSAENSYTEFHIEFGGSSAYYNILDGCKIFYLIPGTSKNWEAYTAWLTSSNDSDKKFLPNMVDVCYCVEVHSQQTILVPSGWIYAVVTPCDTISIAGNFLTFLHIYPQLSIYNLELQLGIEKEYQYPYFESIMWYTAIHFYLAFPDNSSRDGIDDIIAEYETGRLFDINAFTEQELDGFEELLNYLYIRAQILRDCDIIIDIYNEPVKISKNNGYNSAYTMVPPDLDEICVDFVQKFGAWITYHHRRSAKHPSCNCFSHLQTKLIDSGPKPANNSYQHQSNFIGVVISTNHNIIKKCQESQIQTGKNNCSFQLVKKRIKSTKKAPSWRSIIKAFKKRENTRCNFLSSLHATTFREDIVVRPKIKSFVLEQLIFQALFSFAINWTPSFFLNHSNFENIALSKETFNFGGEANCENTDTTLFTTWGDQGFRPSDSICYNDFNLLETANSDAEASIHELELQPLNAVNEREVDISQTDMTPSTALDTRVDTRVDSLPEFSNLILSPSSNDDSFQLDDLLSPSSSNLKQQIQKVVPQNSLEFSVGEKEKKAAEYSLLHTFSYKRLSMENEKPDTTKVPLKYNIQHEEMKAYRRKNDLEYIDQHFASSKSGISNGRNNNKEVNLTKAENVGIKKRRIMKNENNIYDFEDHSPVREKWGHRLRSRGAS cdt2_schpo Cell division cycle protein cdt2 cdt2 SPAC17H9.19c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNMDIGKALSDVGGLRDITSRANNSLPPTPDSSPAAPSKKHKLYDFLESRGKIETPRKRIVFEKKPLLHKPVHIQKKPAQLCKELLIRQLGGSRSHPFSTKTSRHVGGRLNLETYYSRPSECLMMLNQLPFCLGFANNESLLAVCTETGALELFDSRFYDRQNEENQPSARRIHGWLAHNNAIFSVNFSKDDSLLATSSGDQTSKVFDLSTQQCITRLGRRGVDGYHSHSVKQVNFCNDSPYNLVSCSRDGSIIFWDMRTHGITIDGEHFQKPVLRIRKAHENSGRDCSITSATWLPQSTSQVISSCSANSALKLWDLRTVHTVRPLPAATTPELTTSKRDFGVTNVCTSPDGERIYAASRDSIIYEYSSRHLNSGFCKTYKDPRLRISSFYVKLACSPDGATLACGGGVQDKTSGVVVFDTTRNCSSSAMLTGGHTKDVTAVDWSSEGQLASISDDGSVRVWNSSLHGSAANLREKNFSEIFYWGFSEK swi6_schpo Chromatin-associated protein swi6 swi6 SPAC664.01c SPAC824.10c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKKGGVRSYRRSSTSKRSVIDDDSEPELPSMTKEAIASHKADSGSSDNEVESDHESKSSSKKLKENAKEEEGGEEEEEDEYVVEKVLKHRMARKGGGYEYLLKWEGYDDPSDNTWSSEADCSGCKQLIEAYWNEHGGRPEPSKRKRTARPKKPEAKEPSPKSRKTDEDKHDKDSNEKIEDVNEKTIKFADKSQEEFNENGPPSGQPNGHIESDNESKSPSQKESNESEDIQIAETPSNVTPKKKPSPEVPKLPDNRELTVKQVENYDSWEDLVSSIDTIERKDDGTLEIYLTWKNGAISHHPSTITNKKCPQKMLQFYESHLTFRENE F505CC8AC5E75EAB cdt2-jhd1 1013671 n/a 1013673 unspecified role unspecified role prey prey n/a 1013674 unspecified role unspecified role bait bait physical association physical association false false false Fig 3A, 3B swi6-jhd1 1013671 n/a 1013675 unspecified role unspecified role bait bait n/a 1013673 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3A, 3B jhd1-cdt2 1013672 n/a 1013674 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1013673 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3C