UniProt UniProt http://www.uniprot.org jimeno-2011-1 New suppressors of THO mutations identify Thp3 (Ypr045c)-Csn12 as a protein complex involved in transcription elongation. yeast Saccharomyces cerevisiae Baker's yeast tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Jimeno S., Tous C., Garcia-Rubio ML., Ranes M., Gonzalez-Aguilera C., Marin A., Aguilera A. aguilo@us.es Mol. Cell. Biol. (0270-7306) 2011 Only protein-protein interactions imex curation jimeno-2011-2 New suppressors of THO mutations identify Thp3 (Ypr045c)-Csn12 as a protein complex involved in transcription elongation. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jimeno S., Tous C., Garcia-Rubio ML., Ranes M., Gonzalez-Aguilera C., Marin A., Aguilera A. aguilo@us.es Mol. Cell. Biol. (0270-7306) 2011 Only protein-protein interactions imex curation rl7a_yeast 60S ribosomal protein L7-A YGL076C RPL7A RPL6A RPL8A YL8A L6 YL8 RP11 Large ribosomal subunit protein uL30-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MAAEKILTPESQLKKSKAQQKTAEQVAAERAARKAANKEKRAIILERNAAYQKEYETAERNIIQAKRDAKAAGSYYVEAQHKLVFVVRIKGINKIPPKPRKVLQLLRLTRINSGTFVKVTKATLELLKLIEPYVAYGYPSYSTIRQLVYKRGFGKINKQRVPLSDNAIIEANLGKYGILSIDDLIHEIITVGPHFKQANNFLWPFKLSNPSGGWGVPRKFKHFIQGGSFGNREEFINKLVKSMN rl7b_yeast 60S ribosomal protein L7-B RPL6B YL8B RPL7B L6 YL8 RP11 YPL198W Large ribosomal subunit protein uL30-B protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTEKILTPESQLKKTKAQQKTAEQIAAERAARKAANKEKRAIILERNAAYQKEYETAERNIIQAKRDAKAAGSYYVEAQHKLVFVVRIKGINKIPPKPRKVLQLLRLTRINSGTFVKVTKATLELLKLIEPYVAYGYPSYSTIRQLVYKRGFGKINKQRVPLSDNAIIEANLGKYGILSIDDLIHEIITVGPHFKQANNFLWPFKLSNPSGGWGVPRKFKHFIQGGSFGNREEFINKLVKAMN rs13_yeast 40S ribosomal protein S13 RPS13 RPS13C YDR064W YD9609.18 D4252 S27a YS15 Small ribosomal subunit protein uS15 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGRMHSAGKGISSSAIPYSRNAPAWFKLSSESVIEQIVKYARKGLTPSQIGVLLRDAHGVTQARVITGNKIMRILKSNGLAPEIPEDLYYLIKKAVSVRKHLERNRKDKDAKFRLILIESRIHRLARYYRTVAVLPPNWKYESATASALVN csn12_yeast Cop9 signalosome complex subunit 12 J1860 YJR084W CSN12 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDVDIGCYFEEKRYDDKLLDFIRYDVKTPKKTKYILQRPTATDEESVRLQRFYQLGVDLKLKYSKRRSLKKQGRIKNATEELLRLANEQLKLFNRIVERETNWIIYPLWVMAKQLIRLANESSELNKDSIEECGRTIHRSFTICLNDRNPRLNENKKIGCYMFANLEFSIYHRLSNKDMIKNLVKVLESRVNARDIPPLNKSLAMEHKSQVVLYNYYLGQYYGCLENDHERGFFHLNEALLQCPMLYVESTGKFVLQGQMEKIMILLVPLALLTKRLYPHWDHPVIAGVITRSKRLSQVYPTLVRSVISGNLSLYEATAASHERFFLSQGLHVVITLLREVVFTRLVQRCWQWGNDRKSIMPLKILLATKQHDSSANEDEEEQLDALECRLASAIASGLLRAYLSHSNRCIVFSKKEPFPHSK rl3_yeast 60S ribosomal protein L3 MAK8 RPL3 TCM1 YOR063W YOR29-14 YL1 RP1 Maintenance of killer protein 8 Trichodermin resistance protein Large ribosomal subunit protein uL3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSHRKYEAPRHGHLGFLPRKRAASIRARVKAFPKDDRSKPVALTSFLGYKAGMTTIVRDLDRPGSKFHKREVVEAVTVVDTPPVVVVGVVGYVETPRGLRSLTTVWAEHLSDEVKRRFYKNWYKSKKKAFTKYSAKYAQDGAGIERELARIKKYASVVRVLVHTQIRKTPLAQKKAHLAEIQLNGGSISEKVDWAREHFEKTVAVDSVFEQNEMIDAIAVTKGHGFEGVTHRWGTKKLPRKTHRGLRKVACIGAWHPAHVMWSVARAGQRGYHSRTSINHKIYRVGKGDDEANGATSFDRTKKTITPMGGFVHYGEIKNDFIMVKGCIPGNRKRIVTLRKSLYTNTSRKALEEVSLKWIDTASKFGKGRFQTPAEKHAFMGTLKKDL rl8b_yeast 60S ribosomal protein L8-B RPL8B KRB1 RPL4B L4 L4-1 YL5 RP6 YLL045C Large ribosomal subunit protein eL8-B protein protein yeast Saccharomyces cerevisiae Baker's yeast MAPGKKVAPAPFGAKSTKSNKAKNPLTHSTPKNFGIGQAVQPKRNLSRYVKWPEYVRLQRQKKILSIRLKVPPTIAQFQYTLDRNTAAETFKLFNKYRPETAAEKKERLTKEAAAIAEGKSKQDASPKPYAVKYGLNHVVSLIENKKAKLVLIANDVDPIELVVFLPALCKKMGVPYAIIKGKARLGTLVNQKTSAVAALTEVRAEDEAALAKLVSTIDANFADKYDEVKKHWGGGILGNKAQAKMDKRAKTSDSA thp3_yeast Protein THP3 YP9499.03c YPR045C THP3 THO-related protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MQNPYGHFTNNTTEDREASSQGGPFGQSLNRPLDYAGSFPSLTYNNNNFIANQQPSLPLPEPRLSWNNVNQVSNPLMVTPLPGLQKRMNKNIKKKLPRVSKKASALSNGVSGNVMSNSNIVGHGAVGSASGWKVEMGGSDELERRKRRAERFSQGPSATTNSNDNLNEDFANLNAISSKSHQYDKKIHVVGRCQTLEKSYLRLTSEPNPDLIRPPNILQKMYCLLMDKYQSKTATYTYLCDQFKSMRQDLRVQMIENSFTIKVYQTHARIALENGDLGEFNQCQNRIMALFENPTIPKKSYSEFICYSVLYSMLTEDYPSISHLKLKLIDDGSSEILEDEHVKMIFELSDMKLVGNYHYFMKNYLKLHKFEKCLINSFLNLEKLIFLTIICKSYNQVNLDFVKSEFNFNSIEETTNFLNEQNLTEFILNKQITDSNGKSSNIKILNTKGCRVQLIQNYMKSKKIDIKGQK rl8a_yeast 60S ribosomal protein L8-A RPL8A RPL4A L4 L4-2 YL5 RP6 L7a-1 Maintenance of killer protein 7 MAK7 YHL033C Large ribosomal subunit protein eL8-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MAPGKKVAPAPFGAKSTKSNKTRNPLTHSTPKNFGIGQAVQPKRNLSRYVKWPEYVRVQRQKKILSIRLKVPPTIAQFQYTLDRNTAAETFKLFNKYRPETAAEKKERLTKEAAAVAEGKSKQDASPKPYAVKYGLNHVVALIENKKAKLVLIANDVDPIELVVFLPALCKKMGVPYAIVKGKARLGTLVNQKTSAVAALTEVRAEDEAALAKLVSTIDANFADKYDEVKKHWGGGILGNKAQAKMDKRAKNSDSA rs16b_yeast 40S ribosomal protein S16-B YDL083C Small ribosomal subunit protein uS9-B RPS16B RP61R protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAVPSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVEPEILRFKVYEPLLLVGLDKFSNIDIRVRVTGGGHVSQVYAIRQAIAKGLVAYHQKYVDEQSKNELKKAFTSYDRTLLIADSRRPEPKKFGGKGARSRFQKSYR rs16a_yeast 40S ribosomal protein S16-A Small ribosomal subunit protein uS9-A YM9375.12 YMR143W RPS16A RP61R protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAVPSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVEPEILRFKVYEPLLLVGLDKFSNIDIRVRVTGGGHVSQVYAIRQAIAKGLVAYHQKYVDEQSKNELKKAFTSYDRTLLIADSRRPEPKKFGGKGARSRFQKSYR thp3-csn12-1 1157525 n/a 1157527 unspecified role unspecified role prey prey n/a 1157528 unspecified role unspecified role prey prey n/a 1157529 unspecified role unspecified role prey prey n/a 1157530 unspecified role unspecified role prey prey n/a 1157531 unspecified role unspecified role prey prey n/a 1157532 unspecified role unspecified role prey prey n/a 1157533 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1157534 unspecified role unspecified role prey prey n/a 1157535 unspecified role unspecified role prey prey n/a 1157536 unspecified role unspecified role prey prey association association false false false Fig. 7 csn12-yp045 1157526 n/a 1157530 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1157533 unspecified role unspecified role prey prey region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 9