IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ singh-2010-1 Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. human-sk_mel_28 Homo sapiens SK-MEL-28 cell line derived from melanoma sk_mel_28 human SK-MEL-28 Species: human male 51 years old; Tissue: skin; Tumor: melanoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Singh S., Davis R., Alamanda V., Pireddu R., Pernazza D., Sebti S., Lawrence N., Chellappan S. The cells were xenotransplanted to create tumors in athymic nude mice. The tumors were extracted after treatment with DMSO or RD-251 and this tissue was used to perform the coinmunoprecipitation. Srikumar.Chellappan@moffitt.org Mol. Cancer Ther. (1535-7163) 2010 Only protein-protein interactions imex curation singh-2010-2 Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. human-sk_mel_28 Homo sapiens SK-MEL-28 cell line derived from melanoma sk_mel_28 human SK-MEL-28 Species: human male 51 years old; Tissue: skin; Tumor: melanoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Singh S., Davis R., Alamanda V., Pireddu R., Pernazza D., Sebti S., Lawrence N., Chellappan S. The cells were xenotransplanted to create tumors in athymic nude mice. The tumors were extracted after treatment with DMSO or RD-251 and this tissue was used to perform the coinmunoprecipitation. Srikumar.Chellappan@moffitt.org Mol. Cancer Ther. (1535-7163) 2010 Only protein-protein interactions imex curation singh-2010-3 Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. human-sk_mel_2 Human Skin Melanoma cell line 2 sk-mel-2 Human Skin Melanoma cell line 2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Singh S., Davis R., Alamanda V., Pireddu R., Pernazza D., Sebti S., Lawrence N., Chellappan S. The cells were xenotransplanted to create tumors in athymic nude mice. The tumors were extracted after treatment with DMSO or RD-251 and this tissue was used to perform the coinmunoprecipitation. Srikumar.Chellappan@moffitt.org Mol. Cancer Ther. (1535-7163) 2010 Only protein-protein interactions imex curation raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK rb1-raf1 1174552 n/a 1174555 unspecified role unspecified role prey prey n/a 1174556 unspecified role unspecified role bait bait physical association physical association false false false 2b Small molecule RD-251 decreases interaction. raf1-rb1 1174553 n/a 1174556 unspecified role unspecified role prey prey n/a 1174555 unspecified role unspecified role bait bait physical association physical association false false false 2c Small molecule RD-251 decreases interaction. raf1-rb1-1 1174554 n/a 1174556 unspecified role unspecified role prey prey n/a 1174555 unspecified role unspecified role bait bait physical association physical association false false false 2b Small molecule RD-251 decreases the interaction.