InnateDB InnateDB anders-2011-2 HLA-DM captures partially empty HLA-DR molecules for catalyzed removal of peptide. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Anders AK., Call MJ., Schulze MS., Fowler KD., Schubert DA., Seth NP., Sundberg EJ., Wucherpfennig KW. kai_wucherpfennig@dfci.harvard.edu Nat. Immunol. (1529-2908) 2011 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity anders-2011-1 HLA-DM captures partially empty HLA-DR molecules for catalyzed removal of peptide. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Anders AK., Call MJ., Schulze MS., Fowler KD., Schubert DA., Seth NP., Sundberg EJ., Wucherpfennig KW. kai_wucherpfennig@dfci.harvard.edu Nat. Immunol. (1529-2908) 2011 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity dma_human HLA class II histocompatibility antigen, DM alpha chain HLA-DMA DMA RING6 MHC class II antigen DMA Really interesting new gene 6 protein protein protein human Homo sapiens Human MGHEQNQGAALLQMLPLLWLLPHSWAVPEAPTPMWPDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFFFDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFCEWMIQQIGPKLDGKIPVSRGFPIAEVFTLKPLEFGKPNTLVCFVSNLFPPMLTVNWHDHSVPVEGFGPTFVSAVDGLSFQAFSYLNFTPEPSDIFSCIVTHEIDRYTAIAYWVPRNALPSDLLENVLCGVAFGLGVLGIIVGIVLIIYFRKPCSGD a0a1v1igj9_human HLA-DRB1 protein protein human Homo sapiens Human MVCLKFPGGSCMAALTVTLMVLSSPLALAGDTRPRFLEQVKHECHFFNGTERVRFLDRYFYHQEEYVRFDSDVGEYRAVTELGRPDAEYWNSQKDLLEQKRAAVDTYCRHNYGVGESFTVQRRVYPEVTVYPAKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKTGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSLTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS dra_human HLA class II histocompatibility antigen, DR alpha chain HLA-DRA MHC class II antigen DRA HLA-DRA1 protein protein human Homo sapiens Human MAISGVPVLGFFIIAVLMSAQESWAIKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENVVCALGLTVGLVGIIIGTIFIIKGLRKSNAAERRGPL mbp_human Myelin basic protein MBP Myelin A1 protein Myelin membrane encephalitogenic protein protein protein human Homo sapiens Human MGNHAGKRELNAEKASTNSETNRGESEKKRNLGELSRTTSEDNEVFGEADANQNNGTSSQDTAVTDSKRTADPKNAWQDAHPADPGSRPHLIRLFSRDAPGREDNTFKDRPSESDELQTIQEDSAATSESLDVMASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARR dmb_human HLA class II histocompatibility antigen, DM beta chain HLA-DMB DMB RING7 MHC class II antigen DMB Really interesting new gene 7 protein protein protein human Homo sapiens Human MITFLPLLLGLSLGCTGAGGFVAHVESTCLLDDAGTPKDFTYCISFNKDLLTCWDPEENKMAPCEFGVLNSLANVLSQHLNQKDTLMQRLRNGLQNCATHTQPFWGSLTNRTRPPSVQVAKTTPFNTREPVMLACYVWGFYPAEVTITWRKNGKLVMPHSSAHKTAQPNGDWTYQTLSHLALTPSYGDTYTCVVEHIGAPEPILRDWTPGLSPMQTLKVSVSAVTLGLGLIIFSLGVISWRRAGHSSYTPLPGSNYSEGWHIS hg2a_human HLA class II histocompatibility antigen gamma chain DHLAG HLA-DR antigens-associated invariant chain Ia antigen-associated invariant chain CD74 protein protein human Homo sapiens Human MHRRRSRSCREDQKPVMDDQRDLISNNEQLPMLGRRPGAPESKCSRGALYTGFSILVTLLLAGQATTAYFLYQQQGRLDKLTVTSQNLQLENLRMKLPKPPKPVSKMRMATPLLMQALPMGALPQGPMQNATKYGNMTEDHVMHLLQNADPLKVYPPLKGSFPENLRHLKNTMETIDWKVFESWMHHWLLFEMSRHSLEQKPTDAPPKVLTKCQEEVSHIPAVHPGSFRPKCDENGNYLPLQCYGSIGYCWCVFPNGTEVPNTRSRGHHNCSESLELEDPSSGLGVTKQDLGPVPM drb1_human HLA class II histocompatibility antigen, DRB1 beta chain HLA-DRB1 Human leukocyte antigen DRB1 protein protein human Homo sapiens Human MVCLKLPGGSCMTALTVTLMVLSSPLALSGDTRPRFLWQPKRECHFFNGTERVRFLDRYFYNQEESVRFDSDVGEFRAVTELGRPDAEYWNSQKDILEQARAAVDTYCRHNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFLNGQEEKAGMVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS dma-hla_dmb-2 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P28067:p.Arg124Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133491 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133493 competitor competitor competitor neutral component neutral component P02686:p.Pro89Val mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P02686:p.Pro89Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false P02686:p.Pro85Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false P02686:p.Pro89Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 unspecified role unspecified role prey prey region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association false false false Fig. 1B, 1E, 2E, 2F CLIP 15mer is convalently attached via thrombin-cleavable linker at the n' of HLA-DRB injection of MBP peptide at stage 3 caused disassembly of the quaternary complex dma-hla_dmb-3 1133488 n/a 1133490 unspecified role unspecified role bait bait P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P28067:p.Arg124Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role prey prey P01903:p.Phe49Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false P01903:p.Gly53His mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P01903:p.Gly53Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P01903:p.Gln43Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133491 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false Fig. 1B, 1D, 6C, 6F, S2, S9 CLIP 15mer is convalently attached via thrombin-cleavable linker at the n' of HLA-DRB injection of MBP peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-4 1133488 n/a 1133490 unspecified role unspecified role bait bait P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P28067:p.Ile98Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role prey prey P01903:p.Trp68Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association false false false Fig. 1D, 2A, 2B, 2C, 2D, 6C, S9 CLIP 15mer is convalently attached via thrombin-cleavable linker at the n' of HLA-DRB injection of free CLIP peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-19 1133488 n/a 1133490 unspecified role unspecified role bait bait P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P28067:p.Arg124Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false Fig. 3A, S1 injection of CLIP peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-6 1133488 n/a 1133490 unspecified role unspecified role bait bait P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P28067:p.Ile98Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133495 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association false false false Fig. 3A injection of CLIP peptide at stage 3 cause disassembly of the quaternary complex dma-2b11-1 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 unspecified role unspecified role prey prey P04233:p.[Met107Ala;Pro112_Leu113delinsGlyAla;Met115Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association false false false Fig. 3B, 3E, 3F, 4D, 4E, 4F, 5A, 5B, 7A, 7B, 7C, 7D, 7E, 7F, 7G, S8 Fig. 3E, 3F used HLA-DR preloaded with HA (306-318). Fig. 4D, 4E, 4F, 5A, 5B, 7B, 7D, 7E used HLA-DR linked with HA6 or HA3 peptides injection of HA peptide(306-318) at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-8 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133495 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association false false false Fig. 3C, S4, S7A, S7B HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with CLIP (103-117) peptide injection of free CLIP (87-101) or HA (306-318) peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-9 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association false false false Fig. 3C, S7A HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with CLIP (103-117) peptide injection of free CLIP (87-101) or HA (306-318) peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-10 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133493 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false Fig. 3C HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with MBP peptide injection of free CLIP (87-101) peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-11 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133493 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false Fig. 3C HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with MBP peptide injection of free CLIP (87-101) peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-12 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133493 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133493 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false Fig. 3D HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with MBP peptide injection of free MBP(85-99) peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-13 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association false false false Fig. 3E injection of HA peptide(306-318) at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-16 1133488 n/a 1133490 unspecified role unspecified role bait bait P28067:p.[Arg124Ala;Arg220Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P28067:p.Arg124Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false dnp tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role prey prey P01903:p.Gly74Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false P01903:p.Ser78His mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P01903:p.Trp68Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133491 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false Fig. S3 DNP-CLIP 15mer is convalently attached via thrombin-cleavable linker at the n' of HLA-DRB injection of MBP peptide at stage 3 cause disassembly of the quaternary complex dma-hla_dmb-18 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133493 competitor competitor competitor ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133495 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association false false false Fig. S4 HLA-DR2 (HLA-DRA :: HLA-DRB1) is preloaded with CLIP (103-117) peptide injection of free MBP peptide at stage 3 cause disassembly of the quaternary complex dma-2b1f 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false Fig. 3G injection of HA peptide(306-318) at stage 3 cause disassembly of the quaternary complex HLA-DR4 (HLA-DRA :: HLA-DRB4) was preloaded with HA(306-318) peptide dma-hla_dmb-15 1133488 n/a 1133490 unspecified role unspecified role bait bait region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133495 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133494 unspecified role unspecified role prey prey protein c tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133496 unspecified role unspecified role prey prey crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster association association false false false Fig. 3G HLA-DR4 (HLA-DRA :: HLA-DRB4) was preloaded with CLIP peptide injection of free HA peptide(306-318) at stage 3 cause disassembly of the quaternary complex dma-hla_dmb 1133489 n/a 1133490 competitor competitor competitor neutral component neutral component region biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P28067:p.Ile98Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false P28067:p.[Ile98Ala;Pro194Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm n/a 1133492 unspecified role unspecified role neutral component neutral component P01903:p.Gly53His mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P01903:p.Gly53Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false P01903:p.Phe49Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133496 unspecified role unspecified role neutral component neutral component crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133493 unspecified role unspecified role neutral component neutral component region alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1133494 competitor competitor competitor neutral component neutral component protein c tag tag tag range range range range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false Fig. 1A, 1C, 6B, 6D, 6E DRB1*04:01 was used in combination with DRA to assemble HLA-DR2, which then interacted with the MBP peptide. Interaction is disrupted by the presence of HLA-DM, which is made up of HLA-DMA and HLA-DMB. dma-hla_dmb-1 1133489 n/a 1133496 unspecified role unspecified role neutral component neutral component crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133492 unspecified role unspecified role neutral component neutral component P01903:p.Gly53His mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P01903:p.Gly53Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false P01903:p.Phe49Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster n/a 1133493 unspecified role unspecified role neutral component neutral component region alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false association association false false false Fig. 1A (black line) DRB1*04:01 was used in combination with DRA to assemble HLA-DR2, which then interacted with the MBP peptide. Interaction is disrupted by the presence of HLA-DM, which is made up of HLA-DMA and HLA-DMB.