IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ demarco-2010-1 RACK-1 acts with Rac GTPase signaling and UNC-115/abLIM in Caenorhabditis elegans axon pathfinding and cell migration. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Demarco R.S., Lundquist E.A. erikl@ku.edu PLoS Genet. (1553-7390) 2010 Only protein-protein interactions imex curation demarco-2010-2 RACK-1 acts with Rac GTPase signaling and UNC-115/abLIM in Caenorhabditis elegans axon pathfinding and cell migration. caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Demarco R.S., Lundquist E.A. erikl@ku.edu PLoS Genet. (1553-7390) 2010 Only protein-protein interactions imex curation q95qm5_caeel unc-115 F09B9.2 CELE_F09B9.2 protein protein caeel Caenorhabditis elegans MKFSRPVLSVNHLILGKKCDVCRKKCSGDVLKANDKYFHINCFQCKKCGRNLGETGFYTTPENAYLCPDDFRAVSKEITVKTTTQQAHASSSSAATPKSPEKSNGTTDVSSSGAANATLQQISPLGSPTTCAACDQALHSGQVLLALGLSWHVYCFKCSECSAVLHGEYMSHHGKPLCLRDYNEKFGVKCYECEKFIAGKVLQAGGYKFHPTCARCSRCGSHFGDGEEMYMQGDEIWHPSCEHARTTENIAPTGRAATLSRNEPKYQSTFGQHQAYMYLLPEVEQTYLRHPVMNAKEPNAPQYHVPQGPIKIRKSRMSMLKTGMQRLTEDLEKNIPRPKSPHMDNEEPIELAHYPAAHVPDPDTLPAIEREDFPAPQFPYAVEELKRRLSTSSVENEISDDEYSESDKVDEDKLRKTVETLEKYNDSSIAHVIRQNIEDSHKKQRLPLHWDPRNASRTPSGKKMPHLKFRYDVPINASPSRHLNRPRPWVVWQGGERDQGNNTLPCFHIPEDSRANTLRAATLPTEFGQNLSLENLDTTISSHYSEHSTTESGTAGGPRSAGGAVLRSSLPDMSKPAKQYDLLFLQTTNSDLPEDVDRQHLERHLPRDQFEEIFKMSLIEFYKLPEWKRINLKRKHKLF gblp_caeel Guanine nucleotide-binding protein subunit beta-2-like 1 K04D7.1 rack-1 protein protein caeel Caenorhabditis elegans MVQEQMKLTGTLEGHTGWVTQIATYTRNDKTTVLSSSRDKTILVWDVDSVAPVDEGPIGRPVRSLTGHNHFVSDVVISSDGQFALSGSWDKTLRLWDLNQGVSTRQFISHTKDVLSVAFSADNRQIVSGSRDKSIKLWNTLAQCKYTITDDCHTDWVSTVRFSPSNRDPVIVSAGWDKVVKVWNLGNCRLKTNHIGHTGYVNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLPGNDVINAMSFSPNRYWLCAAVGSSIKIWDLEDKKEIEELKPEIASSGSSRGSSPQCISLAWSQDGQTLFAGYTDNIIRVYQVSIRASN unc_115-rack_1 1184374 n/a 1184376 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1184377 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A rack_1-unc_115 1184375 n/a 1184376 unspecified role unspecified role prey prey n/a 1184377 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1B