UniProt UniProt http://www.uniprot.org kumar-2011-1 Hepatitis B virus regulatory HBx protein binds to adaptor protein IPS-1 and inhibits the activation of beta interferon. mouse-liver Mus musculus liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Kumar M., Jung SY., Hodgson AJ., Madden CR., Qin J., Slagle BL. Apoptosis - Interactions involving proteins with a function related to apoptosis bslagle@bcm.edu J. Virol. (0022-538X) 2011 Only protein-protein interactions imex curation kumar-2011-2 Hepatitis B virus regulatory HBx protein binds to adaptor protein IPS-1 and inhibits the activation of beta interferon. human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Kumar M., Jung SY., Hodgson AJ., Madden CR., Qin J., Slagle BL. Apoptosis - Interactions involving proteins with a function related to apoptosis bslagle@bcm.edu J. Virol. (0022-538X) 2011 Only protein-protein interactions imex curation kumar-2011-3 Hepatitis B virus regulatory HBx protein binds to adaptor protein IPS-1 and inhibits the activation of beta interferon. human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Kumar M., Jung SY., Hodgson AJ., Madden CR., Qin J., Slagle BL. Apoptosis - Interactions involving proteins with a function related to apoptosis bslagle@bcm.edu J. Virol. (0022-538X) 2011 Only protein-protein interactions imex curation p20d1_mouse N-fatty-acyl-amino acid synthase/hydrolase PM20D1 Pm20d1 Peptidase M20 domain-containing protein 1 protein protein mouse Mus musculus Mouse MAELLASLPAWAAVLLLFFATVSGSTGPRSRENRGASRIPSQFSEEERVAIKEALKGAIQIPTVSFSHEESNTTALAEFGEYIRKAFPTVFHSSLVQHEVVAKYSHLFTIQGSDPSLQPYMLMAHIDVVPAPEEGWEVPPFSGLERNGFIYGRGALDNKNSVMAILHALELLLIRNYSPKRSFFIALGHDEEVSGEKGAQKISALLQARGVQLAFLVDEGSFILEGFIPNLEKPVAMISVTEKGALDLMLQVNMTPGHSSAPPKETSIGILSAAVSRLEQTPMPNMFGGGPLKKTMKLLANEFSFPINIVLRNLWLFHPIVSRIMERNPITNALVRTTTALTMFNAGIKVNVIPPLAQATINCRIHPSQTVHEVLELVKNTVADDRVQLHVLRSFEPLPISPSDDQAMGYQLLQETIRSVFPEVDIVVPGICIANTDTRHYANITNGMYRFNPLPLNPQDFSGVHGINEKVSVQNYQNQVKFIFEFIQNADTYKEPVPHLHEL a2as03_mouse Helz2 BC006779 protein protein mouse Mus musculus Mouse MASVGCSLRSASTSATNGPSLAGLCAKVDLYLGCSRCTQCLNESTYILREVEHTCPREILLARFKQAAESKIWRKVGRRPSFPTPMRYQVCHYYRPGLGCRRHWNRCTFARSPEEALVWTFELKNNLPRLKLKEAVQGTRAPDRLQTPADTIRAEFGGHFQLLCAICFTCCPPCLCPVDPRGHCPKHQICPTLLIHVIVEGLKRQFVEVRPLPQRRHPLNYCMYVGRGVPCRHGASRCEYAHSAVEMAVWKAEQLDGLQRGDLLTYPLFGENKWKASPNPNPPVTKLYCHACLVTCNSQEAFENHCSSLEHAQMVAFDQAVPWKHRAPPMGLSKFDLCPRPDLCEHGEVCIKAHSKQELQEWVQRAQDMELREQAAWQDGLVPYQARLLAEYQRSSKEVSVMAETIRGVSVTCHPPPVHQAQEKIQHQWVFTIHSEDPLLHVALLNQEPGAAFSLVAPSLPPHQLYAQGKHFCVQSSPAQYKVGVLVQAVAFGSFEQWVVFDFGRRPVLLQKLKLQLGQTHSQGLNGKPAPSHPQELECWHTGNRHVVLEVDWTPEQEALMAKYKLPSLALEFNQIVPDWGPISRSNYRQRMHKFLYEEEAAQQQLVAKLAMKGQVSLKTALETPALGMLFAPPGALYAKVPFHSSLLPDTDQGFLLSRAVSTALVAPVPAPNSTVYQVRLEARASSDHALWLLLPARCCMALGLQAQDSPILEVQFQIDPMTFRFWHQAVDALLEEHLVVPDLPACTLPHPWPTPPSFRGNHKQKLAVGLIAGRRPEGTKHIPPLLIYGPFGTGKTYTLAMAALEVVQQPHTKVLICTHTNSAADIYIREYFHDYVSSGHPEATPLRVMYADRPPRQTDPTTLQYCCLTEDRQAFRPPTGPELVHHRLVVTTTSQARELQVPAGFFSHIFIDEAAQMLECEALIPLSYALSLTRVVLAGDHMQVTPRLFSVPRDKSARHTLLHRLFLYYQQEAHKIAQQSRIIFHENYRSTAAIINFVSHHFYLAKGNPIQASGKVPRHPQHYPLMFCHVAGSPEQDMSMTSWLNSAEVTQVVEKVREIYNTWPHCWGPREQRHICAVSHGAQVSALRQELRRRNLGEVSVGSFEILPGREFRVVVLSSVHNRNSLLSPGAPTSEFFTEPRVLNTVMTRAQSQLVAVGDAVALCSSGACRNLWRSFIRECIEHHSAFPEELSLEQIEQGVAQRQNWASLTLKARGPETEQKSMAQGPQRLIAEGTMVTVKAETRAEAAAKAQTAAVAAEDTASGNSASRDAAAEVSTLEGGMSEEDSESDFWPSDWELNADDAILKELLDESQQVTVTVREDGLLDTVVCSAPQKAREYTNLPSSVLWKFLRSNSKQFRRCSFLQETFERALATPLDDMASSPIQVRGRLNCGMAFTGDEVLVQILGPAGDDRCVPGSLQGRVMGVLKRRRHELAFVCRMDEWDPRIMIPINGSVTKIFVAEMKDPQQVPIHRLIQGQVQRVRHETLKPEDRSTRLFWVRIVLWRERFYYPLGIVLEVLPKAITWEQGLYILDLEHGLKAHTPDPASVSKALQRYRSELNTAAGHREDYRHFLTFTVDPQGACNLDDALSVRDLGPVYEVAVHIADVASLVPKDGALDVEARQQGTVFYAPNREPVLMLPASLCQDALSLLPGQDRLAISLFLTMEKGGGQIKSLRFAPSIIRSDRQLSYEEAEELIKRHPGAGLELPAHLDSVEACVVAACYFSWMLRRQRLSAACYYEPPDEDSVLGFRTAHIMVQEYMIQFNSHVAEFLVSNKHTQTLTPLRWQPTPSRQQLDSVFKKYRGLVPLSLHLCHHSNTDYTPNKQLHLLTSLWKQVQLAAGTQDYSQMVDLIAADDMHPSLAPACLDLRRALGRSVFGRSSQGKQQPAVHHSLQVDWYTWATSPIRRYLDVVLQRLILLALGHRGSTYSNRDIDGLCLDFSRQYASAQSYQRRAYSLHLAIQLKSQPQNKLGFVVDVEMGARCFKVLFPINRETLPDPCPIHYHSLQLAEHPQELVSQTGVRLVWRRRMYSVQASKLPLPLLGTSLDPHTQTVDAALWMKLLMLLKEQRWPEIAALIQEQDKRFHPREKVKIHQSRCGHFVEVVYELGSGDTLQVQLGSSLQRGFLAPTLKLWTVVPGFSLCLEHMERPGDCFSSHVHQALQDQYLQVGEYSGAWGPRCALESLTNAVTENDSIVLHDVHISWDTSQGQLQGTFQLEAAFLQEKCINIHFGCCYLCIRLEGLPLPLDSSLPGPSGLGPFLNIDPNTYTWVAHGLSGDWDHELAGGDWDQENVDDRQEAPKQVYFLIHHMTMEKVPEEVLRPSARFTVEVLSKQLPDLRKEEAVRQLKTASPLVISIALGLPIPEIRWPISGPRRLVSELRWPIPGPRRPVSEPHRPMSGPCGPISEPCRSIPEPCRGNWPRQHSFHKASTSRFLERQNYNIPAGHHKLNQSQDRAVRSALQKQFTVIQGPPGTGKTVVGFHIVYWFHRSNQEQMPTDSSPSGEEQLGGPCVLYCGPSNKSVDVLGGLLLRRKTEMKPLRVYGEQAEATEFPLPGVSNRSLFGKTSQEGRPNQSLRSITLHHRIRQAPNPYAAEIRKFDAQLREGKIFSKEDLRVYRRVLGKARKHELERHSVILCTCSCAASKSLKILNVRQILIDEAGMATEPETLIPLVCFSKTVEKVVLLGDHKQLRPVVKSEQLQSLGMDRSLFERYHRDAIMLDTQYRMHKDICSFPSVEFYGGKLKTWSDLRRLPSILGHTGKPSCSVIFGSVQGHEQKLLVSTEDGNENSRANPEEVTQVVRIIKQLTLDRTVDPKDIAVLTPYNAQAAAISRGLMQRGVTGVTVTSITKSQGSEWRYVIVSTVRTCPRSDVDQRPTKSWLKKFLGFVVDPHQVNVAITRAQEALCIIGDHLLLRCCPLWHRLLDFCEAQHSLVSAEKCDNPTPNPAEVRVAASEIYTRTSGASKAWLQNLL mavs_mouse Mitochondrial antiviral-signaling protein Mavs Ips1 Visa CARD adapter inducing interferon beta Interferon beta promoter stimulator protein 1 Virus-induced-signaling adapter protein protein mouse Mus musculus Mouse MTFAEDKTYKYIRDNHSKFCCVDVLEILPYLSCLTASDQDRLRASYRQIGNRDTLWGLFNNLQRRPGWVEVFIRALQICELPGLADQVTRVYQSYLPPGTSLRSLEPLQLPDFPAAVSGPSAFAPGHNIPDHGLRETPSCPKPVQDTQPPESPVENSEQLLQTNSGAVARMSGGSLIPSPNQQALSPQPSREHQEQEPELGGAHAANVASVPIATYGPVSPTVSFQPLPRTALRTNLLSGVTVSALSADTSLSSSSTGSAFAKGAGDQAKAATCFSTTLTNSVTTSSVPSPRLVPVKTMSSKLPLSSKSTAAMTSTVLTNTAPSKLPSNSVYAGTVPSRVPASVAKAPANTIPPERNSKQAKETPEGPATKVTTGGNQTGPNSSIRSLHSGPEMSKPGVLVSQLDEPFSACSVDLAISPSSSLVSEPNHGPEENEYSSFRIQVDESPSADLLGSPEPLATQQPQEEEEHCASSMPWAKWLGATSALLAVFLAVMLYRSRRLAQ x_hbvcj Protein X X HBx Peptide X pX protein protein hbvcj Hepatitis B virus genotype C subtype ayr MAARLCCQLDPARDVLCLRPVGAESRGRPVSGPFGPLPSPSSSAVPADHGAHLSLRGLPVCAFSSAGPCALRFTSARSMETTVNAHQVLPKVLHKRTLGLSAMSTTDLEAYFKDCLFKDWEELGEEIRLKVFVLGGCRHKLVCSPAPCNFFPSA ank3_mouse Ankyrin-3 Ankyrin-G Ank3 protein protein mouse Mus musculus Mouse MSEEPKEKPAKPAHRKRKGKKSDANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTLKVVTEEIMTTTTITEKHKMNVPETMNEVLDMSDDEVRKASAPEKLSDGEYISDGEEGDKCTWFKIPKVQEVLVKSEDAITGDTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASLRSFSSDRSYTLNRSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVSSPVHSGFLVSFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLAELLNGMDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPSGEGVSNGYKGDATPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLADCHQVLETVGLASQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQENFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQEPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKAEKADRRQSFASLALRKRYSYLTEPSMSPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDGHPSFQVELETPMGLYCTPPNPFQQDDHFSDISSIESPFRTPSRLSDGLVPSQGNIEHPTGGPPVVTAEDTSLEDSKMDDSVTVTDPADPLDVDESQLKDLCQSECAQCWASVPGIPNDGRQAEPLRPQTRKVGMSSEQQEKGKSGPDEEVTEDKVKSLFEDIQLEEVEAEEMTEDQGQAMLNRVQRAELAMSSLAGWQNETPSGSLESPAQARRLTGGLLDRLDDSSDQARDSITSYLTGEPGKIEANGNHTAEVIPEAKAKPYFPESQNDIGKQSIKENLKPKTHGCGRTEEPVSPLTAYQKSLEETSKLVIEDAPKPCVPVGMKKMTRTTADGKARLNLQEEEGSTRSEPKQGEGYKVKTKKEIRNVEKKTH mavs_human Mitochondrial antiviral-signaling protein Interferon beta promoter stimulator protein 1 Virus-induced-signaling adapter CARD adapter inducing interferon beta Putative NF-kappa-B-activating protein 031N MAVS IPS1 KIAA1271 VISA protein protein human Homo sapiens Human MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLPINSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSSENRGLGSELSKPGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPDGGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH x-ank3 1022944 n/a 1022947 unspecified role unspecified role prey prey n/a 1022948 unspecified role unspecified role prey prey n/a 1022949 unspecified role unspecified role prey prey n/a 1022950 unspecified role unspecified role bait bait n/a 1022951 unspecified role unspecified role prey prey physical association physical association false false false Table 1 mavs-x-1 1022945 n/a 1022952 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1022950 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2A x-mavs 1022946 n/a 1022950 unspecified role unspecified role bait bait n/a 1022952 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2B