InnateDB InnateDB glasmacher-2010-2 Roquin binds inducible costimulator mRNA and effectors of mRNA decay to induce microRNA-independent post-transcriptional repression. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy immunostaining immunostaining Glasmacher E., Hoefig KP., Vogel KU., Rath N., Du L., Wolf C., Kremmer E., Wang X., Heissmeyer V. ddx6 and fmr1 were identified via immunofluoresence whereas rc3h1 was identified through tag fluorescence Species of rc3h1 established through correspondence vigo.heissmeyer@helmholtz-muenchen.de species of rc3h1 established through correspondence Nat. Immunol. (1529-2908) 2010 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity glasmacher-2010-1 Roquin binds inducible costimulator mRNA and effectors of mRNA decay to induce microRNA-independent post-transcriptional repression. mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes confocal microscopy confocal microscopy immunostaining immunostaining Glasmacher E., Hoefig KP., Vogel KU., Rath N., Du L., Wolf C., Kremmer E., Wang X., Heissmeyer V. ddx6 and fmr1 were identified via immunofluoresence whereas rc3h1 was identified through tag fluorescence Species of rc3h1 established through correspondence vigo.heissmeyer@helmholtz-muenchen.de Nat. Immunol. (1529-2908) 2010 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity glasmacher-2010-3 Roquin binds inducible costimulator mRNA and effectors of mRNA decay to induce microRNA-independent post-transcriptional repression. mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Glasmacher E., Hoefig KP., Vogel KU., Rath N., Du L., Wolf C., Kremmer E., Wang X., Heissmeyer V. cells were taken from CD4+ T cells from Tg(DO11.10)-Tg(CAR-1) mice. ddx6 and fmr1 were identified via immunofluoresence whereas rc3h1 was identified through tag fluorescence Species of rc3h1 established through correspondence vigo.heissmeyer@helmholtz-muenchen.de Nat. Immunol. (1529-2908) 2010 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity glasmacher-2010-4 Roquin binds inducible costimulator mRNA and effectors of mRNA decay to induce microRNA-independent post-transcriptional repression. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Glasmacher E., Hoefig KP., Vogel KU., Rath N., Du L., Wolf C., Kremmer E., Wang X., Heissmeyer V. ddx6 and fmr1 were identified via immunofluoresence whereas rc3h1 was identified through tag fluorescence Species of rc3h1 established through correspondence vigo.heissmeyer@helmholtz-muenchen.de Nat. Immunol. (1529-2908) 2010 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity glasmacher-2010-5 Roquin binds inducible costimulator mRNA and effectors of mRNA decay to induce microRNA-independent post-transcriptional repression. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Glasmacher E., Hoefig KP., Vogel KU., Rath N., Du L., Wolf C., Kremmer E., Wang X., Heissmeyer V. ddx6 and fmr1 were identified via immunofluoresence whereas rc3h1 was identified through tag fluorescence Species of rc3h1 established through correspondence vigo.heissmeyer@helmholtz-muenchen.de Nat. Immunol. (1529-2908) 2010 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity ddx6_human Probable ATP-dependent RNA helicase DDX6 DDX6 HLR2 RCK DEAD box protein 6 ATP-dependent RNA helicase p54 Oncogene RCK protein protein human Homo sapiens Human MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP rc3h1_mouse Roquin-1 Rc3h1 Gm551 Kiaa2025 RING finger and C3H zinc finger protein 1 Protein Sanroque RING finger and CCCH-type zinc finger domain-containing protein 1 protein protein mouse Mus musculus Mouse MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQIPEQQPITLCSGVEDTKHYEEAKKCVEELALYLKPLSSARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLENGLVAVRTVVHGLVDYIQNHSKKGADQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPSAPILSDESAVDLSNRKPPALPNGIASSGSTVTQLIPRGTDPSFDSSLKPVKLDHLSSSAPGSPPDLLESAPKSISALPVNPHPVPPRGPTDLPPMPVTKPIQMVPRGSQLYPAQQADVYYQDPRGSAPAFETAPYQQGMYYTPPPCVSRFVRPPPSAPEPGPPYLDHYSPYLQDRVINSQYGTQPQQYPPMYPAHYDGRRVYPAQSYTREEMFRESPIPIDIPSAAVPSYVPESRERYQQVEGYYPVAPHPAQIRPSYPRDPPYSRLPPPQPHPSLDELHRRRKEIMAQLEERKVISPPPFAPSPTLPPAFHPEEFLDEDLKVAGKYKANDYSQYSPWSCDTIGSYIGTKDAKPKDVVAAGSVEMMNVESKGTREQRLDLQRRAVETSDDDLIPFGDRPTVSRFGAISRTSKTLYQGAGPLQAIAPQGAPTKSINISDYSAYGAHGGWGDSPYSPHANIPPQGHFIEREKMSMAEVASHGKPLLSAEREQLRLELQQLNHQISQQTQLRGLEAVSNRLVLQREVNTLASQPQPPQLPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSVDMKSKLGTSKQAENGQPEPQNKIRTEDLTLTFSDVPNGSALTQENLSLLSNKTSSLNLSEDSEGGGDNNDSQRSGVVSNSAP edc4_human Enhancer of mRNA-decapping protein 4 Human enhancer of decapping large subunit Autoantigen Ge-1 Autoantigen RCD-8 EDC4 HEDLS protein protein human Homo sapiens Human MASCASIDIEDATQHLRDILKLDRPAGGPSAESPRPSSAYNGDLNGLLVPDPLCSGDSTSANKTGLRTMPPINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAVSNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVNGKIQEEILVHIRQPEGTPLNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHSTWPVDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGYVKFWQIYIEGQDEPRCLHEWKPHDGRPLSCLLFCDNHKKQDPDVPFWRFLITGADQNRELKMWCTVSWTCLQTIRFSPDIFSSVSVPPSLKVCLDLSAEYLILSDVQRKVLYVMELLQNQEEGHACFSSISEFLLTHPVLSFGIQVVSRCRLRHTEVLPAEEENDSLGADGTHGAGAMESAAGVLIKLFCVHTKALQDVQIRFQPQLNPDVVAPLPTHTAHEDFTFGESRPELGSEGLGSAAHGSQPDLRRIVELPAPADFLSLSSETKPKLMTPDAFMTPSASLQQITASPSSSSSGSSSSSSSSSSSLTAVSAMSSTSAVDPSLTRPPEELTLSPKLQLDGSLTMSSSGSLQASPRGLLPGLLPAPADKLTPKGPGQVPTATSALSLELQEVEPLGLPQASPSRTRSPDVISSASTALSQDIPEIASEALSRGFGSSAPEGLEPDSMASAASALHLLSPRPRPGPELGPQLGLDGGPGDGDRHNTPSLLEAALTQEASTPDSQVWPTAPDITRETCSTLAESPRNGLQEKHKSLAFHRPPYHLLQQRDSQDASAEQSDHDDEVASLASASGGFGTKVPAPRLPAKDWKTKGSPRTSPKLKRKSKKDDGDAAMGSRLTEHQVAEPPEDWPALIWQQQRELAELRHSQEELLQRLCTQLEGLQSTVTGHVERALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRLERSIRDEIKKTVPPCVSRSLEPMAGQLSNSVATKLTAVEGSMKENISKLLKSKNLTDAIARAAADTLQGPMQAAYREAFQSVVLPAFEKSCQAMFQQINDSFRLGTQEYLQQLESHMKSRKAREQEAREPVLAQLRGLVSTLQSATEQMAATVAGSVRAEVQHQLHVAVGSLQESILAQVQRIVKGEVSVALKEQQAAVTSSIMQAMRSAAGTPVPSAHLDCQAQQAHILQLLQQGHLNQAFQQALTAADLNLVLYVCETVDPAQVFGQPPCPLSQPVLLSLIQQLASDLGTRTDLKLSYLEEAVMHLDHSDPITRDHMGSVMAQVRQKLFQFLQAEPHNSLGKAARRLSLMLHGLVTPSLP g3bp1_human Ras GTPase-activating protein-binding protein 1 G3BP1 G3BP ATP-dependent DNA helicase VIII GAP SH3 domain-binding protein 1 protein protein human Homo sapiens Human MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ ddx6_mouse Probable ATP-dependent RNA helicase DDX6 Ddx6 Hlr2 Rck ATP-dependent RNA helicase p54 DEAD box protein 6 Oncogene RCK homolog protein protein mouse Mus musculus Mouse MSTARTENPVIMGLSSQNGQLRGPVKASAGPGGGGTQPQPQLNQLKNTSTINNGTPQQAQSMAATIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPAEDEKP edc4_mouse Enhancer of mRNA-decapping protein 4 Edc4 protein protein mouse Mus musculus Mouse MASCASIDIEDATQHLRDILKLDRPAGGSNAESQRPSSAYNGDLNGLLVPDPLSSGDGNSTNKPGIRTMPPINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAVSNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVKGKIQEEILVHIRQPEGTALNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHNTWPVDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGFVKFWQIYIEGQDEPRCLHEWKPHDGRPLSCLLFCDNHKKQDPEVPFWRFLITGADQNRELKMWCTVSWTCLQTIRFSPDIFSSVSVPPSLKVCLDLSAEYLILSDVQRKVLYVMELLQNQDEGRACFSSISEFLLTHPVLSFGIQVVSRCRLRHTEVLPAEEENDSLGTESSHGAGALESAAGVLIKLFCVHTKALQDVQIRFQPQLNPDVVAPLSTHTAHEDFTFGESRPELGSEGLASAAHGSQPDLRRIVELPAPADFLSLSSETKPELMTPDAFMTPTASLQQISASPSSSSSSSSSSSSSSSSSSSSLTAVSAVSSSSAMDPSLPRPPEELTLSPKLQLDGSLTLNSSSSSLQASPRSLLPGLLPGPADKLISKGPGQVSTAASALSLDLQEVEPLGLPQASPSRTRSPDVISSASTALSQDIPEIASEALSRGFGSSVPEGLIEPNSMASAASALHLLSPRPRQGPELGSQLGLDGGPGDGDRHSTPSLLEAALTQEVATPDSQVWPTAPDITRETCSTLTESPRNGLQEKHKSLAFHRPPYHLLQQRDSQDTSAEQSDHDDEVASLASASGGFGSKIPTPRLPSKDWKTKGSPRTSPKLKRKSKKDDGDSAVGSRLTEHQVAEPPEDWPALIWQQQRELAELWHNQEELLQRLCAQLEGLQSTVTDHVERALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRLERSVRDEIKKTVPPCVSRSLEPVAGQLSNSVATKLTAVEGSMKENISKLLKSKNLTDAIARAAADTLQGPMQAAYREAFQSVVLPAFEKSCQAMFQQINDSFRLGTQEYLQQLESHMKSRKAREQEAREPVLAQLRGLVSTLQSATEQMAATVSSSVRAEVQHQLHVAVGSLQESILAQVQRIVKGEVSVALKEQQATVTSSIMQAMRSAAGTPVPSAHLDCQAQQAHILQLLQQGHLNQAFQQALTAADLNLVLYVCETVDPAQVFGQPPCPLSQPVLLSLIQQLASDLGTRSDLKLSYLEEAVMHLDHSDPITRDHMGSVMAQVRQKLFQFLQADPHNSLSKAARRLSLMLHGLVTPSLP ddx6-fmr1-1 1021067 n/a 1021072 unspecified role unspecified role neutral component neutral component n/a 1021073 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Fig. 3A, S1B rc3h1(1-749) mutant showed impaired foci formation in absence of arsenite, but were still able to translocate into stress granules arsenite ddx6-edc4 1021067 n/a 1021074 unspecified role unspecified role neutral component neutral component n/a 1021072 unspecified role unspecified role neutral component neutral component n/a 1021073 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. S2D arsenite edc4 was detected using an antibody against the nuclear p70 s6 kinase a that is known to crossreact with the cytoplasmic edc4 protein ddx6-g3bp1-1 1021067 n/a 1021075 unspecified role unspecified role neutral component neutral component n/a 1021072 unspecified role unspecified role neutral component neutral component n/a 1021073 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Fig. 3A, S1B rc3h1(1-749) mutant showed impaired foci formation in absence of arsenite, but were still able to translocate into stress granules arsenite ddx6-rc3h1 1021068 n/a 1021076 unspecified role unspecified role neutral component neutral component n/a 1021073 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 2D primary CD4+ T cells were stimulated with anti-CD3 and anti-CD28 for 48hrs, many of the rc3h1-enriched foci in CD4+ T cells showed full colocalization with Rck and Fmrp in 62% of the cells arsenite treatment induced cytoplasmic foci formation rc3h1-ddx6-1 1021069 n/a 1021076 unspecified role unspecified role prey prey n/a 1021073 unspecified role unspecified role bait bait n/a 1021077 unspecified role unspecified role prey prey association association false false false Fig. 4A. rc3h1-ddx6-2 1021070 n/a 1021076 unspecified role unspecified role prey prey n/a 1021073 unspecified role unspecified role bait bait n/a 1021077 unspecified role unspecified role prey prey association association false false false Fig. 4B. rc3h1-edc4-3 1021071 n/a 1021073 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1021077 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4C.