IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kasturi-2010-1 The C. elegans sex determination protein MOG-3 functions in meiosis and binds to the CSL co-repressor CIR-1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kasturi P., Zanetti S., Passannante M., Saudan Z., Muller F., Puoti A. alessandro.puoti@unifr.ch Alessandro Puoti Dev. Biol. (0012-1606) 2010 Only protein-protein interactions imex curation kasturi-2010-2 The C. elegans sex determination protein MOG-3 functions in meiosis and binds to the CSL co-repressor CIR-1. caeel-gavid adult Caenorhabditis elegans - pregnant adult gravid_adult pregnant adult anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kasturi P., Zanetti S., Passannante M., Saudan Z., Muller F., Puoti A. alessandro.puoti@unifr.ch Alessandro Puoti Dev. Biol. (0012-1606) 2010 Only protein-protein interactions imex curation kasturi-2010-3 The C. elegans sex determination protein MOG-3 functions in meiosis and binds to the CSL co-repressor CIR-1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kasturi P., Zanetti S., Passannante M., Saudan Z., Muller F., Puoti A. alessandro.puoti@unifr.ch Alessandro Puoti C.elegans two-hybrid library Dev. Biol. (0012-1606) 2010 Only protein-protein interactions imex curation mog1_caeel Probable pre-mRNA-splicing factor ATP-dependent RNA helicase mog-1 mog-1 Masculinization of germline protein 1 Sex determination protein mog-1 K03H1.2 protein protein caeel Caenorhabditis elegans MSDKRADGRLEGTSDTFGGLVIKKKKVEGDSKPTEPSGKSLLGLDRLASTKREHARKRLEDDDDRGVTESVRKGIEKVHEKHRDRDDRGMKYKSRDDDRRRDRDRSERREPSSRRGWKDRSGDQTPRFKVPDTPSRMSWDQDDREGSSRKRNSWDMPTPRGERDRKRYMDSERSISSAWRSERRNRDDEKRRRHRKPEDSVRSVKEEKAEPTFHDDEERAQWEEEQKNLDREWYDNEGAFDDEYNPFNKVSDEFVEKREKQWQEKTQKPRLTVKQQAIKRENELWENNRLHRSGVVAMADELSSIFEDETDENRVTILVQNIVPPFLDGRIVFTKQAQPIIPVVDTTCDMAVSAARGSVAVRKRREVEDRKKAQDKHWELAGSKLGNLMGVKEKKDETADPEDDDSGNYKESHQFASHMKDNEAVSDFAMEKSIKQQREYLPVFACRQKMMNVIRENNVVIIVGETGSGKTTQLAQYLLEDGFGDSGLIGCTQPRRVAAMSVARRVADEMGVDLGQDVGYAIRFEDCTSEKTIIKYMTDGILLRECLGDGSLDQYSAIIMDEAHERSLNTDVLFGLLREVIAKRADLKLIVTSATMDADKFADFFGGNCPTFTIPGRTFPVELFHARTPVEDYVDAAVKQAVTIHLGGMDGDILIFMPGQEDIECTCEMIKEKLGELDEAPPLAVLPIYSQLPSDLQAKIFQRAPGGMRKAIVATNIAETSLTVDGILFVIDPGFCKMKVYNPRIGMDALSIFPVSQASANQRTGRAGRTGPGQCYRLYTERQFKDELLKSTVPEIQRTNLANVVLLLKSLGVDDLLKFHFMDAPPQDNMLNSMYQLWTLGALDNTGQLTPMGRKMVEFPLDPTLSKMLIMSAEMGCSDEVLTIVSMLSVPAIFFRPKGREEEADAKKEKFQVPESDHLTFLNVYIQWRTHKYSAKWCADNYLHVKALKKVREVRAQLKEIMQDLKLPLISNGSEWDIVRKCICSAYFHNAARLKGIGEYVNVRTGIPCFLHPTSALFGMGFMPDYVVYHELIMTAKEYMQCVTAVDAIWLAELGPMFYSIKESKQSRKELKMESVRTVETMEAEMREAQKEMERRKEESDKAFKRPESSRRVVEVGSKSARSERRKLWGL g5ebt5_caeel F52C9.7 mog-3 CELE_F52C9.7 protein protein caeel Caenorhabditis elegans MSKDVSIGWMYEGAKGNVHREDYLLGKKVDKNFEKYSDVVNAQKAEAIDSIVGTRAVFNSGQSTGLKTSSLQKDIIKSEDPFVAVKVREETKRREIMDNPLTKMRLQAALKSMMTSKAEKSKEKKSKKDKKKKKKKRSSSSSDESSDERVERKRRRSPSPKRRRRPSEKPRKRSRSPRRERPRSPKRSREESRKLSRKPSSSRSKSPRRSREDPRKVARKPSRSRSRSAERLRRKSRSPRRSREEPRKMSRSRSRSADKASSSRQFDSHIPRHLQQQRSRHDSSSGSESEHETRSSHRRRNSDDDERQDPRKKSYGLVEMRKRTAEEREEESRAPTKEYKLVKIPTGRGTGNTPREPRRALSEAEKAARLAEMQQDNVKWRDEIRERNVTKGRKEDDEEKEEADKNGYAPSFIRSQMREACDDMTVEKRLQSNKRGVQRAHGYMDRSFAKK mep1_caeel MOG interacting and ectopic P-granules protein 1 M04B2.1 Nuclear zinc finger protein mep-1 protein protein caeel Caenorhabditis elegans MVTADETVLATTTNTTSMSVEPTDPRSAGESSSDSEPDTIEQLKAEQREVMADAANGSEVNGNQENGKEEAASADVEVIEIDDTEESTDPSPDGSDENGDAASTSVPIEEEARKKDEGASEVTVASSEIEQDDDGDVMEITEEPNGKSEDTANGTVTEEVLDEEEPEPSVNGTTEIATEKEPEDSSMPVEQNGKGVKRPVECIELDDDDDDEIQEISTPAPAKKAKIDDVKATSVPEEDNNEQAQKRLLDKLEEYVKEQKDQPSSKSRKVLDTLLGAINAQVQKEPLSVRKLILDKVLVLPNTISFPPSQVCDLLIEHDPEMPLTKVINRMFGEERPKLSDSEKRERAQLKQHNPVPNMTKLLVDIGQDLVQEATYCDIVHAKNLPEVPKNLETYKQVAAQLKPVWETLKRKNEPYKLKMHRCDVCGFQTESKLVMSTHKENLHFTGSKFQCTMCKETDTSEQRMKDHYFETHLVIAKSEEKESKYPCAICEEDFNFKGVREQHYKQCKKDYIRIRNIMMPKQDDHLYINRWLWERPQLDPSILQQQQQAALQQAQQKKQQQLLHQQQAAQAAAAAQLLRKQQLQQQQQQQQARLREQQQAAQFRQVAQLLQQQSAQAQRAQQNQGNVNHNTLIAAMQASLRRGGQQGNSLAVSQLLQKQMAALKSQQGAQQLQAAVNSMRSQNSQKTPTHRSSKLVTTPSHATVGSSSAPTFVCEICDASVQEKEKYLQHLQTTHKQMVGKVLQDMSQGAPLACSRCRDRFWTYEGLERHLVMSHGLVTADLLLKAQKKEDGGRCKTCGKNYAFNMLQHLVADHQVKLCSAEIMYSCDVCAFKCSSYQTLEAHLTSNHPKGDKKTSTPAKKDDCITLDD g5ebe3_caeel cir-1 CELE_F55F8.4 F55F8.4 protein protein caeel Caenorhabditis elegans MGKGFQNFMSKKDFHPSAFRNLKMVWEARQKKSLEDKRQEELRVAYEKEQEILNNKALLGDEKAKMGLSFMYDAPAGMTKREEPKEEPKFEWQRKYQAPREDWAKGNEEIQDQPFGIQVRNVRCCKCHKWGHINTDRECPLFGKSGNFEDEGYANNPSDLIKDLRRRRQEGKAGPSTSKSSAAAATGEDDDEDEWMDHQQLASDMREEHGIKLKSSVLAGMQTDQQLTRMKKEMTEEEMMLEFFNSMTEKEKKKLHKKLMSGADLEDVMKKKSKKEKKKEKKEKRKNKDKKKDKKKNKKSKSRETDDDSDGSDSEDDWKEKSSKRIKREVESSPEYRSKYIKQEVLSEDENSHGRKDSSRKRAHNDSESSNEVRSSERKRSRRDDSPNAKRSIGLKSPERRSSRDRKSSPKQRQDSPVRRRRSPSSIRKESQRVRKHSPEQKRNGRHDSRSVSPVRRRRSPSPDNRQLGSRKQYSPLRRRQSSPMVASPRRRRSPSPERQRKRRSPSDSPPTRRLSTSPIRRRRSPSPNKLPVRRRRHDSGSPDRDGSESPKMWRKKSHK lag1_caeel Suppressor of hairless protein homolog lag-1 CSL transcription factor lag-1 Lin-12 and glp-1 phenotype protein K08B4.1 protein protein caeel Caenorhabditis elegans MFSWRRGDCESQKEENRSEERKGEETIRFPTRSPFHCVLFLLTDGFVLHKPTASPTVGFSPTIFSFYYSRWESSHRISHDESGFCTAKTPLQDSTFTRHPSTSVPSSPSTPRHSGMDYHQSSSVASSESTASTVAAAAAAAAAASLNQHHHPHLYCDDGLLSRSLTDMVSSGGYDSSSSSLSAAASMCYPTPDAYYYHAPPPPPPPQAQQGFSSTDAWLQMQMQPTYHNFGSTVVSTNSPLPSHLLSYGGQPAFADPFYTIGQSTPNTSSFLDTSNSSFGAPSTVVANPMTNYQLAFQAKLGSLHSLIGDSVQSLTSDRMIDFLSNKEKYECVISIFHAKVAQKSYGNEKRFFCPPPCIYLIGQGWKLKKDRVAQLYKTLKASAQKDAAIENDPIHEQQATELVAYIGIGSDTSERQQLDFSTGKVRHPGDQRQDPNIYDYCAAKTLYISDSDKRKYFDLNAQFFYGCGMEIGGFVSQRIKVISKPSKKKQSMKNTDCKYLCIASGTKVALFNRLRSQTVSTRYLHVEGNAFHASSTKWGAFTIHLFDDERGLQETDNFAVRDGFVYYGSVVKLVDSVTGIALPRLRIRKVDKQQVILDASCSEEPVSQLHKCAFQMIDNELVYLCLSHDKIIQHQATAINEHRHQINDGAAWTIISTDKAEYRFFEAMGQVANPISPCPVVGSLEVDGHGEASRVELHGRDFKPNLKVWFGATPVETTFRSEESLHCSIPPVSQVRNEQTHWMFTNRTTGDVEVPISLVRDDGVVYSSGLTFSYKSLERHGPCRIVSNY mog_3-mog_1 1136525 n/a 1136528 unspecified role unspecified role prey prey n/a 1136529 unspecified role unspecified role bait bait physical association physical association false false false 3A mog_3-mog_3-1 1136525 n/a 1136529 unspecified role unspecified role bait bait n/a 1136529 unspecified role unspecified role prey prey physical association physical association false false false 3A mog_3-mep_1-1 1136525 n/a 1136530 unspecified role unspecified role prey prey n/a 1136529 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3A, 3C cir_1-mog_3-1 1136525 n/a 1136531 unspecified role unspecified role bait bait n/a 1136529 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4B, 4C cir_1-mog_1 1136525 n/a 1136531 unspecified role unspecified role bait bait n/a 1136528 unspecified role unspecified role prey prey physical association physical association false false false 4B cir_1-mep_1-1 1136525 n/a 1136530 unspecified role unspecified role prey prey n/a 1136531 unspecified role unspecified role bait bait physical association physical association false false false 4B lag_1-cir_1 1136525 n/a 1136531 unspecified role unspecified role prey prey n/a 1136532 unspecified role unspecified role bait bait physical association physical association false false false 4B mog_3-mep_1-2 1136526 n/a 1136530 unspecified role unspecified role prey prey n/a 1136529 unspecified role unspecified role bait bait physical association physical association false false false 3B mog_3-cir_1-3 1136526 n/a 1136529 unspecified role unspecified role bait bait n/a 1136531 unspecified role unspecified role prey prey physical association physical association false false false 34D cir_1-mog_3 1136526 n/a 1136531 unspecified role unspecified role bait bait n/a 1136529 unspecified role unspecified role prey prey physical association physical association false false false 4D mog_3-cir_1-1 1136527 n/a 1136529 unspecified role unspecified role bait bait n/a 1136531 unspecified role unspecified role prey prey physical association physical association false false false 4A