IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ meloni-2010-1 DAXX is a new AIRE-interacting protein. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation meloni-2010-3 DAXX is a new AIRE-interacting protein. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation meloni-2010-6 DAXX is a new AIRE-interacting protein. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation meloni-2010-2 DAXX is a new AIRE-interacting protein. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it A human thymus Matchmaker cDNA library. J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation meloni-2010-4 DAXX is a new AIRE-interacting protein. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Untagged proteins recognised by anti-bait Ab, and a fluorescently labelled secondary antibody. Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation meloni-2010-5 DAXX is a new AIRE-interacting protein. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Meloni A., Meloni A., Fiorillo E., Corda D., Incani F., Serra M.L., Contini A., Cao A., Rosatelli M.C. Apoptosis - Interactions involving proteins with a function related to apoptosis Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of AIRE construct inferred from sequence length and mutation mapping. rosatelli@unica.it J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation daxx_human Death domain-associated protein 6 DAXX BING2 DAP6 Daxx Fas death domain-associated protein ETS1-associated protein 1 protein protein human Homo sapiens Human MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDPVLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADTPEASLDSGEGPSGMASQGCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDEEEDEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSGPPCKKSRKEKKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDSPSHGLVTSSLCIPSPARLSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD aire_human Autoimmune regulator AIRE APECED Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein protein protein human Homo sapiens Human MATDAALRRLLRLHRTEIAVAVDSAFPLLHALADHDVVPEDKFQETLHLKEKEGCPQAFHALLSWLLTQDSTAILDFWRVLFKDYNLERYGRLQPILDSFPKDVDLSQPRKGRKPPAVPKALVPPPRLPTKRKASEEARAAAPAALTPRGTASPGSQLKAKPPKKPESSAEQQRLPLGNGIQTMSASVQRAVAMSSGDVPGARGAVEGILIQQVFESGGSKKCIQVGGEFYTPSKFEDSGSGKNKARSSSGPKPLVRAKGAQGAAPGGGEARLGQQGSVPAPLALPSDPQLHQKNEDECAVCRDGGELICCDGCPRAFHLACLSPPLREIPSGTWRCSSCLQATVQEVQPRAEEPRPQEPPVETPLPPGLRSAGEEVRGPPGEPLAGMDTTLVYKHLPAPPSAAPLPGLDSSALHPLLCVGPEGQQNLAPGARCGVCGDGTDVLRCTHCAAAFHWRCHFPAGTSRPGTGLRCRSCSGDVTPAPVEGVLAPSPARLAPGPAKDDTASHEPALHRDDLESLLSEHTFDGILQWAIQSMARPAAPFPS daxx_cerae_protein Death domain-associated protein 6 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to O35613 hdac1_cerae_protein Histone deacetylase 1 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to O09106 hdac2_cerae_protein Histone deacetylase 2 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey aire-daxx 1172377 n/a 1172383 unspecified role unspecified role prey prey n/a 1172384 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O43918:p.Trp78Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1 aire-daxx-2 1172377 n/a 1172384 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1172383 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1 aire-daxx-3 1172378 n/a 1172383 unspecified role unspecified role prey prey n/a 1172384 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 1 aire-daxx-4 1172378 n/a 1172384 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1172385 unspecified role unspecified role prey prey physical association physical association false false false Fig 2C hdac1-aire 1172379 n/a 1172384 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1172386 unspecified role unspecified role bait bait physical association physical association false false false Fig. 8 hdac2-aire 1172379 n/a 1172384 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1172387 unspecified role unspecified role bait bait physical association physical association false false false Fig. 8 aire-daxx-1 1172380 n/a 1172384 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1172383 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1172419 1172417 physical association physical association false false false aire-daxx-5 1172381 n/a 1172383 unspecified role unspecified role neutral component neutral component n/a 1172384 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 4 aire-aire-6 1172382 n/a 1172384 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1172384 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 6