IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ serasinghe-2010-2 Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. in vitro In vitro phage display phage display elisa enzyme linked immunosorbent assay ELISA ELISA elisa Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation serasinghe-2010-1 Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. in vitro In vitro phage display phage display nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation serasinghe-2010-3 Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation serasinghe-2010-4 Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation fis1_human Mitochondrial fission 1 protein FIS1 TTC11 FIS1 homolog Tetratricopeptide repeat protein 11 CGI-135 protein protein human Homo sapiens Human MEAVLNELVSVEDLLKFEKKFQSEKAAGSVSKSTQFEYAWCLVRSKYNDDIRKGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS ph1 Peptide 1 group 1, expressed in M13 phage. PMID: 19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro QHTPHPLPPFLI ph3 Peptide 3, group 3, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WKTQTHPFWDDL ph4 Peptide 4, group 1, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro STPNPLPRWLVP ph9 Peptide 9, group 3, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro SGIHAYPHWLDH ph2 peptide 2, group 1, expressed in M13 phage. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro SHKQDPLPWPRF ph5 Peptide 5, group 1, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro KVQLPLPYPFPF ph13 Peptide 13, group 1, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro KHDPLPYPHFLL ph8 Peptide 8, group 2, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro ESSWQDMLYLLR ph7 peptide 7, group 2, expressed in M13 phage. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro HLWEHEMILDLL ph10 Peptide 10, group 2, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro THRWQEELTLLL pep2 peptide 2, group 1, chemically synthesized. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SHKQDPLPWPRF pep13 peptide 13, group 1, chemically synthesized. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KHDPLPYPHFLL pep1 Peptide 1, group 1. PMID:19864624 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) QHTPHPLPPFLI pep4 Peptide 4, group 1, chemically synthesized. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) STPNPLPRWLVP fis1-ph1-1 1179507 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu91Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1179512 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 1c, 1d, 2a and 2b fis1-ph3-2 1179507 n/a 1179511 unspecified role unspecified role bait bait purified purified Q9Y3D6:p.Leu91Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1179513 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 1c, 1d, 2a and 2b fis1-ph4-2 1179507 n/a 1179514 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu91Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1c, 1d, 2a and 2b fis1-ph9-3 1179507 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9Y3D6:p.Leu58Pro mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu42Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu91Pro mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu77Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1179515 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 1179507 1c, 1d, 2a and 2b The units of the EC50 parameter are number of phage units. fis1-ph2-3 1179507 n/a 1179516 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu91Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1c, 1d, 2a and 2b fis1-ph5-2 1179507 n/a 1179511 unspecified role unspecified role prey prey purified purified Q9Y3D6:p.Leu91Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179517 unspecified role unspecified role bait bait infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1c, 1d, 2a and 2b fis1-ph13-2 1179507 n/a 1179511 unspecified role unspecified role bait bait purified purified Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9Y3D6:p.Leu91Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1179518 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 1179507 1c, 1d, 2a and 2b The units of the EC50 parameter are number of phage units. fis1-ph8-2 1179507 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu77Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu91Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu42Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu58Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1179519 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 1179507 1c, 1d, 2a and 2b The units of the EC50 parameter are number of phage units. fis1-ph7-2 1179507 n/a 1179520 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu77Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu91Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1c, 1d, 2a and 2b fis1-ph10-2 1179507 n/a 1179521 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu91Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1c, 1d, 2a and 2b fis1-ph10-4 1179507 n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179521 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179522 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3b fis1-ph4-4 1179507 n/a 1179522 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179514 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3b fis1-ph7-4 1179507 n/a 1179522 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179520 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 physical association physical association false false false 3b fis1-ph1-3 1179507 n/a 1179512 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1179522 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 3b fis1-ph3-4 1179507 n/a 1179522 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179513 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 3b fis1-ph13-4 1179507 n/a 1179522 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179518 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 3b The units of the EC50 parameter are number of phage units. fis1-ph5-3 1179507 n/a 1179522 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179517 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 physical association physical association false false false 3b fis1-ph8-4 1179507 n/a 1179522 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179519 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 3b The units of the EC50 parameter are number of phage units. fis1-ph2-5 1179507 n/a 1179516 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179522 inhibitor inhibitor unspecified role unspecified role purified purified pep2:p.Leu7Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false pep2:p.Pro8Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1179507 3a, 3b, 4b, 4e and 4f fis1-ph7-6 1179507 n/a 1179520 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179523 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 3c fis1-ph1-5 1179507 n/a 1179512 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179523 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3c fis1-ph5-4 1179507 n/a 1179517 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179523 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 3b fis1-ph2-7 1179507 n/a 1179516 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179523 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3c fis1-ph10-6 1179507 n/a 1179523 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179521 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3c fis1-ph4-6 1179507 n/a 1179514 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1179523 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3c fis1-ph3-6 1179507 n/a 1179523 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179513 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3c fis1-ph13-6 1179507 n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179518 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179523 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1179507 4d and 4e The units of the EC50 parameter are number of phage units. fis1-ph8-6 1179507 n/a 1179523 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179519 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3c The units of the EC50 parameter are number of phage units. fis1-ph1-7 1179507 n/a 1179524 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179512 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1179507 4a and 4e fis1-ph4-8 1179507 n/a 1179525 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179514 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 1179507 4c and 4e fis1-ph1-1 1179508 n/a 1179512 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1b fis1-ph2-1 1179508 n/a 1179516 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1b fis1-ph3 1179508 n/a 1179513 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1b fis1-ph4 1179508 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179514 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 1b fis1-ph5 1179508 n/a 1179517 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1b fis1-ph13 1179508 n/a 1179518 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1b fis1-ph7 1179508 n/a 1179520 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1b fis1-ph8 1179508 n/a 1179519 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1b fis1-ph10 1179508 n/a 1179521 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1b fis1-ph9-1 1179508 n/a 1179511 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179515 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction false false false 1b fis1-pep1 1179509 n/a 1179511 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179524 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1179509 5a and 5k fis1-pep2 1179509 n/a 1179511 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu42Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1179522 unspecified role unspecified role prey prey purified purified pep2:p.Leu7Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1179509 5b, 5e, 5g and 5k fis1-pep4 1179509 n/a 1179525 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1179509 5c and 5k fis1-pep13 1179509 n/a 1179511 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1179523 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1179509 5d, 5k and 5h fis1-pep13-1 1179509 n/a 1179511 unspecified role unspecified role bait bait purified purified cytosolic region experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179523 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1179509 5j and 5k fis1-pep2-2 1179509 n/a 1179522 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179511 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1179509 5i and 5k fis1-pep2-4 1179510 n/a 1179511 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1179522 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1179510 5f fis1-pep13-3 1179510 n/a 1179523 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1179511 unspecified role unspecified role neutral component neutral component purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1179510 5f