UniProt UniProt http://www.uniprot.org saita-2009-2 Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Saita S., Shirane M., Natume T., Iemura S., Nakayama KI. nakayak1@bioreg.kyushu-u.ac.jp J. Biol. Chem. (0021-9258) 2009 mimix curation saita-2009-1 Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Saita S., Shirane M., Natume T., Iemura S., Nakayama KI. nakayak1@bioreg.kyushu-u.ac.jp J. Biol. Chem. (0021-9258) 2009 mimix curation saita-2009-3 Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Saita S., Shirane M., Natume T., Iemura S., Nakayama KI. nakayak1@bioreg.kyushu-u.ac.jp J. Biol. Chem. (0021-9258) 2009 mimix curation saita-2009-4 Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Saita S., Shirane M., Natume T., Iemura S., Nakayama KI. nakayak1@bioreg.kyushu-u.ac.jp J. Biol. Chem. (0021-9258) 2009 mimix curation saita-2009-5 Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Saita S., Shirane M., Natume T., Iemura S., Nakayama KI. nakayak1@bioreg.kyushu-u.ac.jp J. Biol. Chem. (0021-9258) 2009 mimix curation saita-2009-6 Promotion of neurite extension by protrudin requires its interaction with vesicle-associated membrane protein-associated protein. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Saita S., Shirane M., Natume T., Iemura S., Nakayama KI. nakayak1@bioreg.kyushu-u.ac.jp J. Biol. Chem. (0021-9258) 2009 mimix curation zfy27_human Protrudin Spastic paraplegia 33 protein SPG33 Zinc finger FYVE domain-containing protein 27 ZFYVE27 protein protein human Homo sapiens Human MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMPLCSLLTCLGLNVLFLTLNEGAWYSVGALMISVPALLGYLQEVCRARLPDSELMRRKYHSVRQEDLQRGRLSRPEAVAEVKSFLIQLEAFLSRLCCTCEAAYRVLHWENPVVSSQFYGALLGTVCMLYLLPLCWVLTLLNSTLFLGNVEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK vapa_human Vesicle-associated membrane protein-associated protein A VAPA VAP33 33 kDa VAMP-associated protein protein protein human Homo sapiens Human MASASGAMAKHEQILVLDPPTDLKFKGPFTDVVTTNLKLRNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGSTVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNTSDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASFRDNVTSPLPSLLVVIAAIFIGFFLGKFIL vapb_human Vesicle-associated membrane protein-associated protein B/C VAPB UNQ484/PRO983 protein protein human Homo sapiens Human MAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVKTTAPRRYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFMVQSMFAPTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTASKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDGLRMRKTVQSNSPISALAPTGKEEGLSTRLLALVVLFFIVGVIIGKIAL tcpd_human T-complex protein 1 subunit delta CCT4 CCTD SRB CCT-delta Stimulator of TAR RNA-binding protein protein human Homo sapiens Human MPENVAPRSGATAGAAGGRGKGAYQDRDKPAQIRFSNISAAKAVADAIRTSLGPKGMDKMIQDGKGDVTITNDGATILKQMQVLHPAARMLVELSKAQDIEAGDGTTSVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGGAPEIELALRLTEYSRTLSGMESYCVRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILKIDDVVNTR tcpe_human T-complex protein 1 subunit epsilon CCT5 CCTE KIAA0098 CCT-epsilon protein protein human Homo sapiens Human MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAVANTMRTSLGPNGLDKMMVDKDGDVTVTNDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQAARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIKVEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE tcpg_human T-complex protein 1 subunit gamma CCT3 CCTG TRIC5 CCT-gamma hTRiC5 protein protein human Homo sapiens Human MMGHRPVLVLSQNTKRESGRKVQSGNINAAKTIADIIRTCLGPKSMMKMLLDPMGGIVMTNDGNAILREIQVQHPAAKSMIEISRTQDEEVGDGTTSVIILAGEMLSVAEHFLEQQMHPTVVISAYRKALDDMISTLKKISIPVDISDSDMMLNIINSSITTKAISRWSSLACNIALDAVKMVQFEENGRKEIDIKKYARVEKIPGGIIEDSCVLRGVMINKDVTHPRMRRYIKNPRIVLLDSSLEYKKGESQTDIEITREEDFTRILQMEEEYIQQLCEDIIQLKPDVVITEKGISDLAQHYLMRANITAIRRVRKTDNNRIARACGARIVSRPEELREDDVGTGAGLLEIKKIGDEYFTFITDCKDPKACTILLRGASKEILSEVERNLQDAMQVCRNVLLDPQLVPGGGASEMAVAHALTEKSKAMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRLLTSLRAKHTQENCETWGVNGETGTLVDMKELGIWEPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQGGAPDAGQE zfy27_mouse Protrudin Zinc finger FYVE domain-containing protein 27 Zfyve27 protein protein mouse Mus musculus Mouse MQTSDRDLSGPEASPSGMPEVLSECPPAPTKSAAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMPLCSLLTCLGLNILFLTLNEGAWYSMGALMISVPALLGYLQEVCRGQLPESELMRRKYHSIRQEDLQRVRLSRVHLSRPEAVAEVKSFLIQLEAFLARLCYTCESAYRVLHWENPVVSSQFYGALLGMVCMLYLLPLCWVLALLNSTLFLGNGDFFRVVCEYRACLQRRMNPRQEECACESSALQGAGGRGLLDSSPAPTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDEEGTPCPAEDELTLQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCAGCAATFSVLKKRRSCSNCGNSFCSRCCSFKVPRSSMGATAPEAQRETVCVCASCNQTLSK vapa_mouse Vesicle-associated membrane protein-associated protein A 33 kDa VAMP-associated protein Vapa Vap33 protein protein mouse Mus musculus Mouse MASASGAMAKHEQILVLDPPSDLKFKGPFTDVVTTNLKLQNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGSIVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNISDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLNDMEPSKAVPLNASKQDGPLPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSAVSFRDNVTSPLPSLLVVIAAIFIGFFLGKFIL zfy27-vapa-1 1143771 n/a 1143777 unspecified role unspecified role bait bait n/a 1143778 unspecified role unspecified role prey prey physical association physical association false false false 1A and 3B zfy27-vapb-1 1143771 n/a 1143777 unspecified role unspecified role bait bait n/a 1143779 unspecified role unspecified role prey prey physical association physical association false false false 1A zfy27-cct3-5 1143772 n/a 1143777 unspecified role unspecified role bait bait n/a 1143778 unspecified role unspecified role prey prey n/a 1143779 unspecified role unspecified role prey prey n/a 1143780 unspecified role unspecified role prey prey n/a 1143781 unspecified role unspecified role prey prey n/a 1143782 unspecified role unspecified role prey prey physical association physical association false false false table 1 zfy27-vapa 1143773 n/a 1143783 unspecified role unspecified role bait bait n/a 1143784 unspecified role unspecified role prey prey physical association physical association false false false 1B zfy27-vapa-3 1143774 n/a 1143777 unspecified role unspecified role bait bait n/a 1143778 unspecified role unspecified role prey prey physical association physical association false false false 2B vapa-zfy27 1143775 n/a 1143777 unspecified role unspecified role prey prey n/a 1143778 unspecified role unspecified role bait bait direct interaction direct interaction false false false 3E vapa-zfy27-1 1143776 n/a 1143777 unspecified role unspecified role neutral component neutral component n/a 1143778 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4A