UniProt UniProt http://www.uniprot.org serrano-2008-1 Processing of a membrane protein required for cell-to-cell signaling during endospore formation in Bacillus subtilis. bacsu Bacillus subtilis comigration in gel comigration in gel electrophoresis comigration in gel anti tag western anti tag western blot anti tag western Serrano M., Vieira F., Moran C.P., Henriques A.O. Proteins were cross-linked in vivo with DSP prior to purification. aoh@itqb.unl.pt J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation serrano-2008-2 Processing of a membrane protein required for cell-to-cell signaling during endospore formation in Bacillus subtilis. bacsu Bacillus subtilis crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Serrano M., Vieira F., Moran C.P., Henriques A.O. Proteins were cross-linked in vivo with DSP prior to purification. Proteins were cross-linked in vivo with DSP prior to purification. aoh@itqb.unl.pt J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation misca_bacsu Membrane protein insertase MisCA misCA Foldase YidC 2 Membrane integrase YidC 2 Membrane protein YidC 2 Stage III sporulation protein J spoIIIJ BSU41040 protein protein bacsu Bacillus subtilis MLLKRRIGLLLSMVGVFMLLAGCSSVKEPITADSPHFWDKYVVYPLSELITYVAKLTGDNYGLSIILVTILIRLLILPLMIKQLRSSKAMQALQPEMQKLKEKYSSKDQKTQQKLQQETMALFQKHGVNPLAGCFPILIQMPILIGFYHAIMRTQAISEHSFLWFDLGEKDPYYILPIVAGVATFVQQKLMMAGNAQQNPQMAMMLWIMPIMIIVFAINFPAALSLYWVVGNLFMIAQTFLIKGPDIKKNPEPQKAGGKKK miscb_bacsu Membrane protein insertase MisCB misCB Foldase YidC 1 Membrane integrase YidC 1 Membrane protein YidC 1 yqjG BSU23890 protein protein bacsu Bacillus subtilis MLKTYQKLLAMGIFLIVLCSGNAAFAATNQVGGLSNVGFFHDYLIEPFSALLKGVAGLFHGEYGLSIILVTIIVRIVVLPLFVNQFKKQRIFQEKMAVIKPQVDSIQVKLKKTKDPEKQKELQMEMMKLYQEHNINPLAMGCLPMLIQSPIMIGLYYAIRSTPEIASHSFLWFSLGQSDILMSLSAGIMYFVQAYIAQKLSAKYSAVPQNPAAQQSAKLMVFIFPVMMTIFSLNVPAALPLYWFTSGLFLTVQNIVLQMTHHKSKKTAALTESVK sp3ae_bacsu Stage III sporulation protein AE spoIIIAE BSU24390 protein protein bacsu Bacillus subtilis MKRFQWVLLLAVLIIAGRAEIVQAAGNAEQTEDHAETAEQLAERTAASLETDKIGEFWNDIMTEYGGLLPESQKGSLMEFINGDKSFSPQEWLKALFSYLFHEVLANGKLLGTLILLTIFCVILQLLQNAFQQSTVSKVAYSIVYMVLIILALNSFHVAINYATEAIQTMTSFILALIPLLLALLASSGGAVSAAFFHPVILFLMNTSGLLIQNIVMPLIFLSAILSIVSTMTEQYKVTQLANLLRNIAIGALAVFLTIFLGVISVQGASAAVTDGITLRTAKFITGNFIPVLGRMFTDATDTVISASLLLKNTVGILGVAILICIAAFPAIKVLSLAFIYKLAAAILQPLGGGPVITCLDVISKSVIYIFAALAIVSLMFFLSLTVIITAGNLTMMMK oxaa1 1243068 n/a 1243070 unspecified role unspecified role neutral component neutral component c-terminal tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag anti tag western anti tag western blot anti tag western c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false Stoichiometry: 2.0 physical association physical association false false false Fig 2A oxaa2-1 1243068 n/a 1243071 unspecified role unspecified role neutral component neutral component c-terminal tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag anti tag western anti tag western blot anti tag western c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false Stoichiometry: 2.0 physical association physical association false false false Fig 2A spoiiij-spoiiiae 1243069 n/a 1243070 unspecified role unspecified role bait bait c-terminal tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag anti tag western anti tag western blot anti tag western c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false n/a 1243072 unspecified role unspecified role prey prey physical association physical association false false false Fig 2C yqjg-spoiiiae 1243069 n/a 1243072 unspecified role unspecified role prey prey n/a 1243071 unspecified role unspecified role bait bait c-terminal tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag anti tag western anti tag western blot anti tag western c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false physical association physical association false false false Fig 2C