UniProt UniProt http://www.uniprot.org dahl-2007-1 The transcriptional repressor GFI-1 antagonizes PU.1 activity through protein-protein interaction. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dahl R., Iyer S.R., Owens K.S., Cuylear D.D., Simon M.C. Protein identity: gfi-1 was identified as human from atcc image clone no; pu.1 as mouse from ref 23 rao-1999 RDahl@salu.unm.edu RDahl@salud.unm.edu J. Biol. Chem. (0021-9258) 2007 mimix curation dahl-2007-3 The transcriptional repressor GFI-1 antagonizes PU.1 activity through protein-protein interaction. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Dahl R., Iyer S.R., Owens K.S., Cuylear D.D., Simon M.C. Protein identity: gfi-1 was identified as human from atcc image clone no; pu.1 as mouse from ref 23 rao-1999 RDahl@salu.unm.edu RDahl@salud.unm.edu J. Biol. Chem. (0021-9258) 2007 mimix curation dahl-2007-2 The transcriptional repressor GFI-1 antagonizes PU.1 activity through protein-protein interaction. human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dahl R., Iyer S.R., Owens K.S., Cuylear D.D., Simon M.C. Protein identity: gfi-1 was identified as human from atcc image clone no; pu.1 as mouse from ref 23 rao-1999 RDahl@salu.unm.edu RDahl@salud.unm.edu J. Biol. Chem. (0021-9258) 2007 mimix curation spi1_mouse Transcription factor PU.1 Spi1 Sfpi1 Sfpi-1 31 kDa-transforming protein SFFV proviral integration 1 protein protein protein mouse Mus musculus Mouse MLQACKMEGFSLTAPPSDDLVTYDSELYQRPMHDYYSFVGSDGESHSDHYWDFSAHHVHNNEFENFPENHFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHTGLSHQVSYMPRMCFPYQTLSPAHQQSSDEEEGERQSPPLEVSDGEADGLEPGPGLLHGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRLPPH gfi1_human Zinc finger protein Gfi-1 Growth factor independent protein 1 Zinc finger protein 163 GFI1 ZNF163 protein protein human Homo sapiens Human MPRSFLVKSKKAHSYHQPRSPGPDYSLRLENVPAPSRADSTSNAGGAKAEPRDRLSPESQLTEAPDRASASPDSCEGSVCERSSEFEDFWRPPSPSASPASEKSMCPSLDEAQPFPLPFKPYSWSGLAGSDLRHLVQSYRPCGALERGAGLGLFCEPAPEPGHPAALYGPKRAAGGAGAGAPGSCSAGAGATAGPGLGLYGDFGSAAAGLYERPTAAAGLLYPERGHGLHADKGAGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK gfi1_mouse Zinc finger protein Gfi-1 Gfi1 Gfi-1 Growth factor independent protein 1 protein protein mouse Mus musculus Mouse MPRSFLVKSKKAHSYHQPRSPGPDYSLRLETVPAPGRAEGGAVSAGESKMEPRERLSPDSQLTEAPDRASASPNSCEGSVCDPCSEFEDFWRPPSPSVSPASEKSLCRSLDEAQPYTLPFKPYAWSGLAGSDLRHLVQSYRQCSALERSAGLSLFCERGSEPGRPAARYGPEQAAGGAGAGQPGRCGVAGGATSAAGLGLYGDFAPAAAGLYERPSTAAGRLYQDHGHELHADKSVGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK spi1_human Transcription factor PU.1 SPI1 31 kDa-transforming protein protein protein human Homo sapiens Human MLQACKMEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSPAQPSSDEEEGERQSPPLEVSDGEADGLEPGPGLLPGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRHPPH spi1-gfi1 1164524 n/a 1164527 unspecified role unspecified role bait bait n/a 1164528 unspecified role unspecified role prey prey physical association physical association false false false 3a gfi1-spi1 1164524 n/a 1164527 unspecified role unspecified role prey prey n/a 1164528 unspecified role unspecified role bait bait physical association physical association false false false 3a gfi1-spi1-1 1164525 n/a 1164529 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) n/a 1164527 unspecified role unspecified role prey prey in vitro In vitro physical association physical association false false false 3c spi1-gfi1-3 1164525 n/a 1164527 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) n/a 1164529 unspecified role unspecified role prey prey in vitro In vitro physical association physical association false false false 3d spi1-gfi1-2 1164526 n/a 1164528 unspecified role unspecified role prey prey n/a 1164530 unspecified role unspecified role bait bait physical association physical association false false false 3b