IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kim-2007e-1 The Caenorhabditis elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim Y., Kipreos ET. ekipreos@cb.uga.edu Mol. Cell. Biol. (0270-7306) 2007 Only protein-protein interactions imex curation kim-2007e-2 The Caenorhabditis elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Kim Y., Kipreos ET. ekipreos@cb.uga.edu Mol. Cell. Biol. (0270-7306) 2007 Only protein-protein interactions imex curation kim-2007e-3 The Caenorhabditis elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim Y., Kipreos ET. ekipreos@cb.uga.edu Mol. Cell. Biol. (0270-7306) 2007 Only protein-protein interactions imex curation kim-2007e-4 The Caenorhabditis elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kim Y., Kipreos ET. ekipreos@cb.uga.edu Mol. Cell. Biol. (0270-7306) 2007 Only protein-protein interactions imex curation cul4_caeel Cullin-4 F45E12.3 cul-4 protein protein caeel Caenorhabditis elegans MTSGAPPTISTEKSRSKQLRKSSKRTASTVEGTEAKQMRGDTENRSDGEMDDVSTSSEIRHGNGFIENFRSQTGNSSRTTATNERIKKKLVIKNFKANANQNDVAMGDTNIDSTDGSVGRDWAVLSDNVFAILEDRKTVTTLEGLFSKVRSVCDKNQSKVLYDRLVAIVVQFAKSLKESLNAVEQVPLAEDNCEQYLEKFGQIWQAYPVKINLIRNIFLHLDRIALGATDTEILPLWECFMQIFQKTFFPNIFKEFKATKLFNALYMAMQKIMQRYPVDSPLRSLVDMLQTVHVSEEFAKFLISQLREHYNNERIDKVPKMNCNDYMEYCEDQINRYSQLVKVNFDEPSALKDVQATVTNCLIQQAIPEILTHDFDDLIDSDNISDIGRMFNLCRQCVGGEDEVRTQFSKYLKKRGEKLIATCPDEDLVSELLAFKKKVDFIMTGSFKSANDPVKMRQCLSDAFESFVNKQVDRSAELISKHFHTLLHSSNKNVSDDTTLDQMVDEAIVLFRYLRGKDVFEAYYKRGLAKRLFLERSASVDAEKMVLCKLKTECGSAFTYKLEGMFKDMDASENYGRLFNQYLEHMNKEKANFTARVITPEYWPTYDTYEINIPKEMRDTLTDYQDFYRVQHGNRNVKWHHGLASAVISASFRPGCKKELIATMYQTVILLLFNKCETWTVAEIVEHTKILEIEVVKNVLALLGGRDKPKVLQRVEGGGSEKKEGTVENLKNEKFVVNSKFTEKRCRVRIAQVNIKTAVEETKEVKEEVNSDRQYKIDAAVVRIMKARKQLNHQTLMTELLQQLRFPVSTADIKKRLESLIEREYISRDPEEASSYNYVA ddb1_caeel DNA damage-binding protein 1 M18.5 ddb-1 Damage-specific DNA-binding protein 1 protein protein caeel Caenorhabditis elegans MPISYCVSAKKASVVVESVVGNFTGHENVNLIVARGNRIDVQLVSPEGLKNVCEIPIYGQVLTIALVKCKRDKRHSLIVVTEKWHMAILAYRDGKVVTRAAGCIADPTGRATDNLFSLTIHRNGLIAIRAFEGSVKMIQWESGTDLRHFNVRFDYPNVSDFKFVDTGEDDVYRVAFIYDDDHGKHLQFSDLNMHDKEFRTYSRQASIAADSSVLIPVPHAIGGVIVLGSNSVLYKPNDNLGEVVPYTCSLLENTTFTCHGIVDASGERFLLSDTDGRLLMLLLNVTESQSGYTVKEMRIDYLGETSIADSINYIDNGVVFVGSRLGDSQLIRLMTEPNGGSYSVILETYSNIGPIRDMVMVESDGQPQLVTCTGADKDGSLRVIRNGIGIDELASVDLAGVVGIFPIRLDSNADNYVIVSLSDETHVLQITGEELEDVKLLEINTDLPTIFASTLFGPNDSGIILQATEKQIRLMSSSGLSKFWEPTNGEIISKVSVNAANGQIVLAARDTVYLLTCIVDEMGALDIQLTAEKKFENEIACLDLSNEGDDPNNKATFLVLAFWSTFAMEVIQLPDLITVCHTDLPTKIIPRSIIATCIEEVHYLLVAFGDGALVYYVFDIKTGTHGEPKKSNVGTRPPSLHRVRNKNRQHLFVCSDRPVIIFSASKKLVFSNVNVKLVDTVCSLSSSAYRDCLVISDGNSMVFGTVDDIQKIHVRSIPMGESVLRIAYQKSTSTYGVCSNRTESKAERVFASKNALVTSQSRPKVASTRADMDESPPNTTSSFMVLDQNTFQVLHSHEFGPWETALSCISGQFTNDSSTYYVVGTGLIYPDETETKIGRIVVFEVDDVERSKLRRVHELVVRGSPLAIRILNGKLVAAINSSIRLFEWTTDKELRLECSSFNHVIALDLKVMNEEVAVADVMRSVSLLSYRMLEGNFEEVAKDWNSQWMVTCEFITAESILGGEAHLNLFTVEVDKTRPITDDGRYVLEPTGYWYLGELPKVMTRSTLVIQPEDSIIQYSQPIMFGTNQGTIGMIVQIDDKWKKFLIAIEKAIADSVKNCMHIEHSSYRTFVFQKRAEPPSGFVDGDLVESILDMDRSVAMDILSKVSDKGWDPSLPRDPVEILKVIEDLARMH q86s68_caeel Y54E10A.15 cdt-1 CELE_Y54E10A.15 protein protein caeel Caenorhabditis elegans MSSRVTRSRSQTPQTAVTDFFKTTRKPRARGEKKIIDESITKTTTIKEVQIPAAPIVENKSSRTRARTPSRQAELSSSGTPPKKAREAEAEAPELPKIHEDIILENPAPQKSAIDKLMEASGPAKKKAGIRTVADLQARIAAKGAAKAIHEQNLKNKTKQVDDHVELLKSPKKPVIVEHHKSPQKSKAARNLFSPKKPVPDYIIPGFNVLKSAEKVRGEQDDAHEEEGKRMTAEFLKSSRLIDEVKESARQRIELPTAYEHLADTFKRIDQITAIFIGQNRSCVVPELIKNVKNTSGKDFSNEHLSQILHVYPQSYHIEMREQRKAFGQGGKYELEVRPNLVEDLRGYIYEKSPTDGDTEDLPLVCPSKLLSPKKSPRKQVQPALRKPEVDGRIRLDAARQRDRAHVFRYKLASIVTKCHTEFLESQGLCASSNIKRMHPLFRLDKHCPSLPQVKLPEPPLQKSSIHIGMKEALDKYMDQVSLPSFVQKAYQDLKSPLKSTTGASGTVPISPKKFSEMQKEQKSQGAMSLLERIRAKEAIKKAAEACIDKDLERRKQRLTLLRDRYVRIVCNHYTAKRAQTMEIDVICKFVQFSSSNSSSIPDIMDHLRLLCEVAPMYISEASLMNKKYFRFTDNNIEAINELLDEELQITQKKIDEQRNTQIAQMSHHHTPRPSKAARSLKFH cul_4-ddb_1-1 1145111 n/a 1145115 unspecified role unspecified role bait bait n/a 1145116 unspecified role unspecified role prey prey physical association physical association false false false Fig 1D q86s68-ddb_1 1145112 n/a 1145117 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1145116 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 7A ddb_1-q86s68 1145113 n/a 1145116 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1145117 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 7A q86s68-ddb_1-1 1145114 n/a 1145117 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1145116 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 7C