IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ asahina-2006-1 Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation asahina-2006-2 Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation asahina-2006-3 Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation asahina-2006-4 Crosstalk between a nuclear receptor and beta-catenin signaling decides cell fates in the C. elegans somatic gonad. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Asahina M., Valenta T., Silhankova M., Korinek V., Jindra M. masako@paru.cas.cz, jindra@entu.cas.cz Dev. Cell (1534-5807) 2006 Only protein-protein interactions imex curation nhr25_caeel Nuclear hormone receptor family member nhr-25 nhr-25 F11C1.6 protein protein caeel Caenorhabditis elegans MTDVERMVLRPNHEGEMCPVCGDRVSGYHYGLLTCESCKGFFKRTVQNKKQYQCSAEANCHVDRTCRKRCPSCRFQKCLTMGMKMEAVRADRMRGGRNKFGSFYKKDRAHRMQRNAMRVSTVQVPAVLGAQSQAQTFYQPPEHQVSSSTTDQNNQINYFDQTKIKTEYIKTEYDAHLQSPTLSSSTNQQLSVSDFIMRPGYLVDPQDSLAVLLGSTIDDPLLRHTFPAAYQLNEVKQEPFDYSEQFIHHSLHDYPTYTSNTTNYATMMPMTTVSSTQSLVTSTSSTTTGRMTEASSTSPILPLCPAPTEKTVDHFYNSSIAEMCKTLPDDAQIARIFTSVKGTSKPEKHAFSIQVAEENLKDIVIWAKNDQLFSKLSLDDQMILLQTSWTTVHIVDITNAMVHGNLLSQYKMSNGDEVPVGLVALLGNQTFVSSWNDVVIRLRNMGFTNFDYCAFRFLALFDQSMDSFPAVSTARSRVLQSWREVRCTTAFLEIFEQIRRLAYDSLRYLWNLHSNCPTNWEQFFPEASLVLEMIRTTVNRSASSSVTAITQVPAIQLPTPQATYTAVPYMAS wrm1_caeel Armadillo repeat-containing protein wrm-1 wrm-1 Worm armadillo protein 1 B0336.1 protein protein caeel Caenorhabditis elegans MDVDCAETFSQPCTPLNFNPMTPSTSRVSTPVRPSSTMSARQYSGSPFKAQPQNMEPSNSRVQELREAAVGKRSYTNAWMQGTYAPPQMAGQQQRFSRPPSVIGSTMSHMTNMSEMTAYSYGGLSMLSVNTEMGEFNNFVNQAPYQRALTRVSQVSENQDPTNRQYPMTAPEIIENLESTELINQAAAIRALEPIVKAGGMLQTWGPKGAEPIIRALFQVLIPRPVENENVIRKAFEILHHSILLSNKEIRRIDRMFFRLNAALMDPNGPPVFNVPKPYSIYEIVMTRAIQLDTKFESSAMVLLVHLCCKPHFMKIFFGEDETQQSPAHRRLHKIVIEFAIGNLRRPETKSKNKGLCVSIIKNLSNKNATIKDMSERLGVVSLFHQIMQNEVIHEDLLWSTMQALTVFCGDVKNGTHFVQMGGAQVLCGLLSHGSTRLLHELLKCLRRVSDLPAIQEQDMKESIHCIVQLIGCSDVTIVELATGTLRNIGLHNKMNKAFMVQDGVTSHAIAVLRTSEQFTYQPHANIDLYRKQILSIYENCLSVLNNVTSMAPQDIKESAVSACRMISENADSAYVLLHYFNVGNRKCRKLAVTVMKRVIETVPAFADPFVDLLGTTNEPLPILLLQRAFQSLDEWRKTSVEMMNCDGRSAEQRRELDDRRKDHEDIVKRSVGLLTNLCSQANPRFFHSLKLVLTNGTLNPFQWLTHEMSDGILQEWLAFILSICSRDESLQTFMMYRFLEQAKMTEAFFAELKARRQNSNIQTMLSKIIDLGRHQQRIVSQQHQQHQMQHHRQLM nlk_caeel Serine/threonine kinase NLK nlk W06F12.1 lit-1 Nemo-like kinase Loss of intestine protein 1 protein protein caeel Caenorhabditis elegans MVSWGRGKDAYYLYISREQEEDDDDSLSFYSSQKDTEDEFCGCLFPDPEIPGSSSSSGCSSSSTELYDLAAAHAALISRQQQILSQAIPIIPEHQLAAVAAHHQHHQQLHPSVQYQLVAAATHHNHHQPQAAQPHYSAVVPRSDVIQQPPHFALHHHLQNLVQQQQQQQAHHHHQQLVGEMALVSHTHPAAVGSTTCYEKNQQKQQQVQQIPTQPQVAHVSSNAILAAAQPFYPPPVQDSQPDRPIGYGAFGVVWSVTDPRSGKRVALKKMPNVFQNLASCKRVFREIKMLSSFRHDNVLSLLDILQPANPSFFQELYVLTELMQSDLHKIIVSPQALTPDHVKVFVYQILRGLKYLHTANILHRDIKPGNLLVNSNCILKICDFGLARTWDQRDRLNMTHEVVTQYYRAPELLMGARRYTGAVDIWSVGCIFAELLQRKILFQAAGPIEQLQMIIDLLGTPSQEAMKYACEGAKNHVLRAGLRAPDTQRLYKIASPDDKNHEAVDLLQKLLHFDPDKRISVEEALQHRYLEEGRLRFHSCMCSCCYTKPNMPSRLFAQDLDPRHESPFDPKWEKDMSRLSMFELREKMYQFVMDRPALYGVALCINPQSAAYKNFASSSVAQASELPPSPQAW pop1_caeel Protein pop-1 pop-1 W10C8.2 Posterior pharynx defect protein 1 protein protein caeel Caenorhabditis elegans MMADEELGDEVKVFRRDEDADDDPMISGETSEQQLADDKKEAVMEAELDGAGRNPSIDVLKSAFPKVEPMSPSFPGLMSHFSPGYSAAALPMFMPLFMNPYAAALRSPSLMFPMGAMSPTFPMFPPSPVYGAAIAAAAAKQHFENMAPLNMRAGHPMNQMGMPPYMHPSSMAPQNVDRRAQGGGKAKKDDHVKKPLNAFMWFMKENRKALLEEIGNNEKQSAELNKELGKRWHDLSKEEQAKYFEMAKKDKETHKERYPEWSARENYAVNKKKTKKRRDKSIPSENNDQKKCRARFGVNNTEMWCKFCKRKKKCEYATDRSGGSDITDSQDGRGTSGAYSSSSESPSPKANAGIALTTQQQQAAMMHTMLMQMRLGSTTGASTHVPSPLASSSAGRSPLDANASDSESDVEEEEDEQIDPTVMQQTHDMLMQESMCTI q9xvi2_caeel sys-1 T23D8.9 CELE_T23D8.9 protein protein caeel Caenorhabditis elegans MHSTGEPQRGPAGPYHHPMPYHVDQIPTTSHPSWHQAPHPGLPTPPYHPQQHPNYISMQPPPSQQQQMTPQALSTQQQQQVQQQQQRQLYSSPSPSRGPAAPAQNQKFRTEQWINNQAWPYSQSPAPPVAPSVMSYHQGDDRMSMLSVNTTMTNQFPDSQRCYSSNGSTCENVNPEMMQMNITQAAEQAIRLWFNTPDPMQRLHMAKTIRTWIRQDKFAQVDQANMPNCVQQILNIIYDGLKPQPVQLPISYYAQLWYNLLDILRRFTFLPIISPYIHQVVQMFCPRENGPQDFRELICNLISLNWQKDPHMKHCANQVFQIFNCIIMGVKNEKLRTEFAQHLKFEKLVGTLSEYFNPQVHPGMINPAIFIIFRFIISKDTRLKDYFIWNNNPHDQPPPPTGLIIKLNAVMIGSYRLIAGQNPETLPQNPELAHLIQVIIRTFDLLGLLLHDSDAIDGFVRSDGVGAITTVVQYPNNDLIRAGCKLLLQVSDAKALAKTPLENILPFLLRLIEIHPDDEVIYSGTGFLSNVVAHKQHVKDIAIRSNAIFLLHTIISKYPRLDELTDAPKRNRVCEIICNCLRTLNNFLMMWIPTPNGETKTAGPNEKQQVCKFIEIDILKKLMSCLSCEGMDTPGLLELRSTILRSFILLLRTPFVPKDGVLNVIDENRKENLIGHICAAYSWVFRQPNNTRTQSTKQQLVERTISLLLVLMEQCGAEKEVAQYSYSIDCPLNLLNGNQVKPTFIHNVLVVCDKILEHCPTRADIWTIDRPMLEGLTNHRNSDIAKAANSLLSRFPENDLLAGIFSNREYF wrm1-nhr25 1136302 n/a 1136306 unspecified role unspecified role prey prey region-minus dna binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1136307 unspecified role unspecified role bait bait minus_c_terminal 23 amino_acids sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 4A lit_1-wrm_1-1 1136302 n/a 1136307 unspecified role unspecified role prey prey n/a 1136308 unspecified role unspecified role bait bait physical association physical association false false false pop_1-sys_1-1 1136302 n/a 1136309 unspecified role unspecified role bait bait n/a 1136310 unspecified role unspecified role prey prey physical association physical association false false false sys_1-nhr-25 1136303 n/a 1136310 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1136306 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig 4B wrm_1-nhr25 1136303 n/a 1136306 unspecified role unspecified role prey prey 35s-met 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 1136307 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 4B nhr25-wrm_1 1136304 n/a 1136306 unspecified role unspecified role bait bait n/a 1136307 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 4C nhr25-q9xvi2 1136305 n/a 1136306 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false delta- ligand binding domain required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1136310 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 4D